Mass spectrometric analysis of crosslinker of aging proteins formed by Maillard reaction

美拉德反应形成的老化蛋白交联剂的质谱分析

• 批准号: 04836009
• 负责人: NIWA Toshimitsu
• 金额: $ 1.22万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04836009/
• 关键词: Maillard reaction; 3-deoxyglucosone; gas chromatography; mass spectrometry; diabetes mellitus; diabetic nephropathy; タンパク質; 架橋重合; グルコース; 質量分析法; 電気泳動; HPLC

3-Deoxyglucosone, a potent protein crosslinking intermediate of the Maillard reaction, was first detected in diabetic serum using gas chromatography/mass spectrometry. Serum concentration of 3-deoxyglucosone was elevated in diabetic patients as compared with healthy subjects. More notably, diabetic patients with nephropathy showed higher serum concentration of 3-deoxyglucosone than those without nephropathy. The increased serum concentration of 3-deoxyglucosone in the diabetic patients suggests that 3-deoxyglucosone may be responsible for the development of diabetic comlications such as diabetic nephropathy by promoting the formation of advanced glycation end products.

3-脱氧葡糖醛酮，一种有效的蛋白质交联中间体的美拉德反应，首次检测到糖尿病患者血清中使用气相色谱/质谱。糖尿病患者血清3-脱氧葡萄糖醛酮浓度高于健康人。更值得注意的是，糖尿病肾病患者的血清3-脱氧葡萄糖酮浓度高于无肾病。糖尿病患者血清中3-脱氧葡萄糖醛酸浓度升高提示3-脱氧葡萄糖醛酸可能通过促进晚期糖基化终产物的形成而导致糖尿病并发症如糖尿病肾病的发生。

项目成果

Toshimitsu NIWA: "Presence of 3-deoxyglucosone,a potent protein crosslinking intermediate of Maillard Reaction,in diabetic serum." Biochem Biophys Res Commun. 196. 837-843 (1993)

Toshimitsu NIWA：“糖尿病血清中存在 3-脱氧葡萄糖醛酮，一种美拉德反应的有效蛋白质交联中间体。”

Niwa, T., Takeda, N., Yoshizumi, H., Tatematsu, A., Ohara, M., Tomiyama, S., Niimura, K.: "Presence of 3-deoxyglucosone, a potent protein crosslinking intermediate of Maillard Reaction, in diabetic serum" Biochem. Biophys. Res. Commun. 196. 837-843 (1993)

Niwa, T.、Takeda, N.、Yoshizumi, H.、Tatematsu, A.、Ohara, M.、Tomiyama, S.、Niimura, K.：“存在 3-脱氧葡萄糖酮，一种美拉德反应的有效蛋白质交联中间体