Advancement of the Functions of Molecular Assemblies Composed of Amphiphilic Molesules

两亲性分子组成的分子组装体功能的研究进展

• 批准号: 05453095
• 负责人: HARADA Makoto
• 金额: $ 4.29万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05453095/
• 关键词: Anionic surfacatnts; Non-ionic surfactants; Molecular assemblies; Microemulsion; Solubilization of proteins; Control of microemulsions; concentrated surfactant solution; Ultrafine Particles; 分子集合体機能化; 超微粒子形成; アフィニティリガンド; 界面活性剤; 分子集合体組織制御; タンパ

We performed the studies on advancing the functions of a microemulsion which is a kind of molecular assemblies composed of amphiphilic molecules. The obtained results are as follows.1. Functions of unit molecular assembly : We elucidated the fundamental items for advancing the functions of unit assembly ; (1) theoretical model relating the surfactant molecular form with the geometry of the assembly, (2) the behaviors of the surfactant systems yielding spherical and cylindrical assemblies, (3) the characteristics of the solubilization of guest molecules into the host assemblies, (4) the characteristics of the assemblies in non-ionic surfactant systems and in non-ionic and anionic mixed surfactant systems, and (5) the roles of the assemblies in the preparation of CdS fine particles.2. Functions of surfactant/protein systems : We elucidated the driving force for solubilizing proteins in the assemblies and the solubilization dynamics of guest molecules. Based on these, we proposed the methods of tailoring molecular assemblies for protein solubilization and selective separation of proteins without denaturation of proteins. The methods are based on regulating the electrostatic and affinity interactions between proteins and surfactants.3. Functions of concentrated assembly systems : We elucidated the static and dynamic characteristics both of the spherical assembly system composed of concentrated Aerosol-OT and of the network micro-structures in concentrated sodium bis (2ethylhexyl) phosphate system. We also obtained fundamental information on the solidification of nano-structures in microemulsions.

本文对由两亲性分子组成的分子组装体--微乳液的功能进行了研究。主要研究结果如下：1.单元分子组装体的功能：阐明了提高单元组装体功能的基本条件;（1）将表面活性剂分子形式与组装体几何形状相关联的理论模型，（2）产生球形和圆柱形组装体的表面活性剂体系的行为，（3）客体分子溶解到主体组装体中的特性，（4）非离子表面活性剂体系和非离子与阴离子混合表面活性剂体系中组装体的特性;（5）组装体在CdS微粒制备中的作用.表面活性剂/蛋白质系统的功能：我们阐明了增溶蛋白质的驱动力和客体分子的增溶动力学。在此基础上，我们提出了在不变性的条件下定制分子组装体以增溶蛋白质和选择性分离蛋白质的方法。这些方法是基于调节蛋白质和表面活性剂之间的静电和亲和相互作用.浓缩组装体系的功能：阐述了浓缩气溶胶-OT组成的球形组装体系和浓缩磷酸二（2-乙基己基）钠体系的网络微观结构的静态和动态特性。我们还获得了在微乳液中的纳米结构的固化的基本信息。

项目成果

M.Adachi,M.Harada,R.Nishita,and A.shioi: "Extraction Klnetics for Small Charged Molecules in Water-in-oil Microemulsion/Brine System" Journal of Physical Chemistry. 99(in press). (1995)

M.Adachi、M.Harada、R.Nishita 和 A.shioi：“油包水微乳液/盐水系统中小带电分子的萃取动力学”物理化学杂志。

M.Adachi: "Time Dependence of the Solubilization State of Cytochrome c in AOT Water-in-Oil Microemulsion" Journal of Colloid and Interface Science. 164 in press. (1994)

M.Adachi：“AOT 油包水微乳液中细胞色素 c 溶解状态的时间依赖性”胶体与界面科学杂志。

A.Shioi, M.Harada and M.Tanabe: "Effects of Organic Solvents on the Aggregates's Geometry and Winsor II/III Transition in the Sodium Bis (2ethylhexyl) Phosphate System" Journal of Physical Chemistry. 97. 8281-8288 (1993)

A.Shioi、M.Harada 和 M.Tanabe：“有机溶剂对双（2乙基己基）磷酸钠体系中聚集体几何形状和 Winsor II/III 转变的影响”物理化学杂志。

M.Adachi, and M.Harada: "Time-dependence of the Solubilization State of Cytochromec in AOT Water-in-Oil Microemulsion" Journal of Colloid and Interface Science. 165. 229-235 (1994)

M.Adachi 和 M.Harada：“AOT 油包水微乳液中细胞色素溶解状态的时间依赖性”胶体与界面科学杂志。

M.Harada, M.Adachi, A.Shioi, K.Kawakami and K.Kurumada: "Microemulsions as Liquid Media for Material Separation" 2nd International Conference on Materials Enginnering for Resources, ICMR'94 Akita. 248-255 (1994)

M.Harada、M.Adachi、A.Shioi、K.Kawakami 和 K.Kurumada：“微乳液作为材料分离的液体介质”第二届国际资源材料工程会议，ICMR94 秋田。