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IMMUNOLOGIC FUNCTION OF LIVER SINUSOIDAL CELLS

肝正弦细胞的免疫功能

基本信息

批准号：
3079018
负责人：
Dwain Louis Thiele
金额：
$ 7.62万
依托单位：
UT SOUTHWESTERN MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-04-01 至 1987-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3079018
关键词：
Kupffer's cell antigens cell type gene expression human tissue immunogenetics liver cells lymphocyte major histocompatibility complex postmortem surface antigens tissue /cell culture

项目摘要

A variety of non-lymphocyte accessory cells have been shown to play a crucial role in the induction and regulation of immune responses. While the liver contains a large number of candidate accessory cells, little is known about their role in local or systemic immune responses. In the proposed studies, human liver sinusoidal cells will be isolated and cultured. The various cell types within this population will be characterized by a number of histochemical stains, morphological criteria, cell surface receptor assays, and staining with monoclonal antibodies. The ability of these cells to serve as accessory cells in in vitro assays of immune responsiveness will be examined. Interleukin 1 secretion by Kupffer cells or other sinusoidal cells will be quantitated. The potential for immunomudalatory interactions between various members of the hepatic non-parenchymal cell population will be explored. An established technique for isolation of guinea pig liver sinusoidal cells will be utilized to examine in vivo antigen processing by these cells. In this model, antigen taken up in vivo by liver accessory cells will be assayed for immunogenicity as determined by activation of antigen primed T lymphocytes in vitro. By this method, the identity of cells within the liver which are capable of presenting antigen will be determined, and the modulation of such antigen presenting ability by immunologic stimuli will be examined. Thus, this proposal will examine the immunologic potential of liver sinusoidal cells and seek to elucidate mechanisms by which such function is modulated within the microenvironment of the liver.

多种非淋巴细胞附属细胞已被证明发挥作用

项目成果

期刊论文数量（13）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Phenotypic and functional characterization of human T cell clones.

人类 T 细胞克隆的表型和功能特征。

DOI：
发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
0
作者：
Patel,SS;Duby,AD;Thiele,DL;Lipsky,PE
通讯作者：
Lipsky,PE

Regulation of cellular function by products of lysosomal enzyme activity: elimination of human natural killer cells by a dipeptide methyl ester generated from L-leucine methyl ester by monocytes or polymorphonuclear leukocytes.

溶酶体酶活性产物对细胞功能的调节：单核细胞或多形核白细胞通过 L-亮氨酸甲酯产生的二肽甲酯消除人类自然杀伤细胞。

DOI：
10.1073/pnas.82.8.2468
发表时间：
1985
期刊：
Proceedings of the National Academy of Sciences of the United States of America
影响因子：
11.1
作者：
Thiele,DL;Lipsky,PE
通讯作者：
Lipsky,PE

The immunosuppressive activity of L-leucyl-L-leucine methyl ester: selective ablation of cytotoxic lymphocytes and monocytes.