Randomazied Intervention Study of beta-carotene, Chlorophyll and Catechin on Health Effects : a study on utilizing on biological makers such as mutagenicity, natural killer activity and sister chromatid exchange.

β-胡萝卜素、叶绿素和儿茶素对健康影响的随机干预研究：利用致突变性、自然杀伤活性和姐妹染色单体交换等生物标志物的研究。

• 批准号: 05454219
• 负责人: HIROHATA Tomio
• 金额: $ 4.93万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454219/
• 关键词: beta-carotene; Male smokers; Lifestyle; Biomarker; Natural killer activity; Micronuclei test; Sister chromatid exchange; Intervention study; biomarker; 姉妹染色分体交換(SCE); 小核試験(MN); クロロフィル; 喫煙; 無作為介入試験; ベータカロテン; 姉妹染色分体交換; ナチュラルキラー活性

About 200 volunteers of smokers who consumed 15 or more cigarettes a day were divided at random into three groups, beta-carotene (30 mg/day), chlorophyll (120 mg/day) and control (1 mg beta-carotene/day) groups. They were followed for six months with biochemical and other measurement of blood at three months intervals. The baseline (at the initiation of the study) examination indicates that vegetables and fruits intake were positively related to beta-carotene and other carotenoid levels. Tofu intake was unexpectedly, but strongly related to decreased the levels of beta-carotene and cryptoxanthine. Alcohol consumption was strongly and inversely associated with the levels of various carotenoids.As regards the effects of beta-carotene and chlorophyll, natural killer (NK) activities tended to decrease in 3 and 6 months later after the baseline examination for the two groups as well as for the control group.The frequencies of sister chromatid exchange (SCE) and micronuclei (MN) decreased in 3 and 6 months later for the two study groups, but the control groups showed similar tendency.Thus, the study did not provide clear-cut effects of beta-carotene and chlorophyll administration on selected biomarkers such as NK activities, SCE and MN frequencies.

大约200名每天吸烟15支或更多的吸烟者志愿者被随机分为三组，β-胡萝卜素（30毫克/天），叶绿素（120毫克/天）和对照组（1毫克β-胡萝卜素/天）。他们被随访了六个月，每隔三个月进行一次生化和其他血液测量。基线（研究开始时）检查表明，蔬菜和水果摄入量与β-胡萝卜素和其他类胡萝卜素水平呈正相关。豆腐的摄入出乎意料地，但强烈相关的β-胡萝卜素和隐黄质的水平下降。饮酒量与各种类胡萝卜素的水平呈强烈的负相关。至于β-胡萝卜素和叶绿素的影响，自然杀伤细胞（NK）活性在基线检查后3个月和6个月均呈下降趋势，姐妹染色单体交换（SCE）和微核（MN）频率在基线检查后3个月和6个月均呈下降趋势。因此，本研究没有提供β-胡萝卜素和叶绿素施用对所选生物标志物如NK活性、SCE和MN频率的明确影响。

项目成果

Y.Kitamura, K.Tanaka, C.Kiyohara, T.Hirohata, Y.Tomita, M.Ishibashi, K.Kido: "Relationship of alcohol use, physical activity and dietnry habitys with serum carotenoids, retinol and alpha-tocopnerol among male Japanese smokers." Int. J.Epidemiol. 26. 307-3

Y.Kitamura、K.Tanaka、C.Kiyohara、T.Hirohata、Y.Tomita、M.Ishibashi、K.Kido：“男性饮酒、体力活动和饮食习惯与血清类胡萝卜素、视黄醇和 α-生育酚的关系

Y.Kitamura, K.Tanaka, C.Kiyohara, T.Hirohata, Y.Tomita, M.Ishibashi and K.Kido: "Relationship of alcohol use, physical activity and dietary habits with serum carotenoids, retional and alpha-tocopherol among male Japanese smokers." Int.J.Epdemiol.26 (2). 3

Y.Kitamura、K.Tanaka、C.Kiyohara、T.Hirohata、Y.Tomita、M.Ishibashi 和 K.Kido：“男性饮酒、体力活动和饮食习惯与血清类胡萝卜素、视网膜和 α-生育酚的关系