Randomazied Intervention Study of beta-carotene, Chlorophyll and Catechin on Health Effects : a study on utilizing on biological makers such as mutagenicity, natural killer activity and sister chromatid exchange.

大约200名每天吸烟15支或更多的志愿者被随机分为三组，β-胡萝卜素组(30毫克/天)、叶绿素组(120 mg/天)和对照组(1毫克/天)。每隔3个月对他们进行6个月的血液生化和其他测量。基线(在研究开始时)检查表明，蔬菜和水果的摄入量与β-胡萝卜素和其他类胡萝卜素水平呈正相关。豆腐的摄入量出人意料，但与β-胡萝卜素和隐黄碱水平的下降密切相关。饮酒与各种类胡萝卜素水平呈显著负相关。关于β-胡萝卜素和叶绿素的影响，两组和对照组在基线检查后3个月和6个月自然杀伤(NK)活性有下降趋势。姐妹染色单体互换(SCE)和微核(MN)频率在3个月和6个月后下降，但对照组表现出类似的趋势。因此，本研究没有明确提供β-胡萝卜素和叶绿素对NK活性、姐妹染色单体互换(SCE)和微核(MN)等选定生物标志物的影响。

About 200 volunteers of smokers who consumed 15 or more cigarettes a day were divided at random into three groups, beta-carotene (30 mg/day), chlorophyll (120 mg/day) and control (1 mg beta-carotene/day) groups. They were followed for six months with biochemical and other measurement of blood at three months intervals. The baseline (at the initiation of the study) examination indicates that vegetables and fruits intake were positively related to beta-carotene and other carotenoid levels. Tofu intake was unexpectedly, but strongly related to decreased the levels of beta-carotene and cryptoxanthine. Alcohol consumption was strongly and inversely associated with the levels of various carotenoids.As regards the effects of beta-carotene and chlorophyll, natural killer (NK) activities tended to decrease in 3 and 6 months later after the baseline examination for the two groups as well as for the control group.The frequencies of sister chromatid exchange (SCE) and micronuclei (MN) decreased in 3 and 6 months later for the two study groups, but the control groups showed similar tendency.Thus, the study did not provide clear-cut effects of beta-carotene and chlorophyll administration on selected biomarkers such as NK activities, SCE and MN frequencies.