Elucidation and Control of Adhesion Cascade in Lymphocyte Homing

淋巴细胞归巢中粘附级联的阐明和控制

基本信息

批准号：
05454214
负责人：
MIYASAKA Masayuki
金额：
$ 4.1万
依托单位：
Osaka University (1994-1995)Tokyo Metropolitan Organization for Medical Research (1993)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

Previous work by others and ourselves indicate that a variety of adhesion molecules are involved in the interaction between lymphocytes and HEV (high endothelial venules) in lymph nodes and that they mediate an adhesion cascade so that only lymphocytes can recirculate through lymph nodes. In this study, we searched for ligands for L-selectin and investigated the role of L-selectin in this phenomenon. Our results are summarized as follows ;1. By the use of recombinant soluble L-selectin, 180kDa molecule was obtained from rat lymph node lysates. This molecule is apparently different from previously identified L-selectin ligands such as GlyCAM-1 and CD34as judged by the molecular size.2. The 180kDa molecule was purified and its N-termnal amino acid sequence was determined.3. The analysis indicated that this molecule is hightly homologous to human mannose receptor which is mainly expressed in macrophages.4. By the use of polyclonal antiserum against rat mannose receptor, it was revealed that mannose receptor is expressed in medullary macrophages but not in HEV.Therefore, it is unlilely that the 180kDa molecule is involved in the adhesion cascade occurring between lymphocytes and HEV.Separately, we examined if L-selectin can transduce signal, and found out that this molecule can indeed transmit outside-in signal, although it has only 13 amino acid recidues intracellularly. This indicates that L-selectin has an associated molecule (s) with signal transducing ability.

他人和我们自己的先前工作表明，淋巴结中的淋巴细胞与HEV（高内皮静脉静脉）之间的相互作用涉及各种粘附分子，并且它们可以通过淋巴细胞来通过淋巴结循环淋巴细胞介导粘附为Cyscade。在这项研究中，我们搜索了L-选择素的配体，并研究了L-选择素在该现象中的作用。我们的结果总结如下; 1。通过使用重组可溶性L-选择素，从大鼠淋巴结裂解物中获得了180KDA分子。该分子显然与先前鉴定的L-选择蛋白配体（如糖-1和CD34A）不同的分子有所不同。2。纯化了180kDa的分子，并确定其N-期限氨基酸序列3。分析表明，该分子与人类甘露糖受体同源，主要在巨噬细胞中表达。4。 By the use of polyclonal antiserum against rat mannose receptor, it was revealed that mannose receptor is expressed in medullary macrophages but not in HEV.Therefore, it is unlilely that the 180kDa molecule is involved in the adhesion cascade occurring between lymphocytes and HEV.Separately, we examined if L-selectin can transduce signal, and found out that this molecule can实际上，尽管它的细胞内只有13个氨基酸循环，但它确实会传输外部信号。这表明L-选择素具有与信号转导能力的相关分子。