EVALUATION OF SELECTIVE INTERNAL RADIATION THERAPY WITH I-131-LIPIODOL IN HEPATOCELLULAR CARCINOMA OF RAT

I-131-碘油选择性内照射治疗大鼠肝细胞癌的评价

• 批准号: 05454304
• 负责人: HAMAMOTO Ken
• 金额: $ 4.22万
• 依托单位: EHIME UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454304/
• 关键词: I-131-LIPIODOL; HEPATOCELLULAR CARCINOMA; I-131; LIPIODOL; INTERNAL RADIATION THERAPY; TRANSARTERIAL EMBOLIZATION; RAT LIVER CANCER MODEL; ^<131>I(放射性ヨード); 内部照射; ラット担癌モデル; ^<131>I-リピオドール; 栓塞療法; ^<131>I; 肝癌治療; オートラジオグラフィ; ヨード131リピオドール; 肝細胞癌

Hepatocellular carcinoma is the 3rd most common cancer in Japan. So far effective treatments have not been established. Since the liver has a dual blood supply and hepatic tumors derive mainly from hepatic artery, intra-arterial treatments give a chance of selectively targeting the lesions. As a chemotoxic agents or radio-iodine, Lipiodol has been used for the treatment of hepatic cancer. The purposes of this study are : -1.To establish the naturally occurred hepatic cancer model in rat.2.To establish the technique to label iodine-131 with Lipiodol.3.To estimate the biodistribytion of transarterially administered iodine-131-labeled Lipiodol and to evaluate the radiation effects both on tumor and normal tissues by microscopically and radiographically.Results : -1.Hepatic carcinoma developed in rats after 20 to 24 weeks by feeding 3'-Me-DAB.2.According Shin's method, stable (more than 95% labeled) I-131-Lipiodol was obtained.3.Seven days after injection of I-131-Lipiodol, it was taken exclusively in the tumor and disappeared from normal liver tissue and lung where a small amount of radioactivity accumulated one day after injection.4.The necrotic area in the tumor was seen 21 days after injection.5.The levels of liver enzymes and blood cells remained within statistic fluctuations.Clinical use of this therapy would be expected because of it's effects on the tumor and tolerable side effects.

肝细胞癌是日本第三大常见癌症。到目前为止，还没有有效的治疗方法。由于肝脏具有双重血液供应，并且肝脏肿瘤主要来自肝动脉，因此动脉内治疗提供了选择性靶向病变的机会。碘油作为化学毒性剂或放射性碘，已用于治疗肝癌。本研究的目的是：1.建立大鼠肝癌模型; 2.建立碘-131碘油标记技术; 3.通过显微镜和放射学方法，评价碘-131碘油经动脉给药后的生物分布，以及对肿瘤和正常组织的辐射效应。1.大鼠经3 '-Me-DAB喂养20 ~ 24周后发生肝癌。2.根据Shin的方法，（超过95%标记）获得I-131-碘油。3.注射I-131-碘油后7天，4.注射后第21天肿瘤内可见坏死区; 5.肝酶和肝纤维化指标在注射后第21天开始下降，第21天开始下降。血细胞保持在统计学波动范围内，该疗法对肿瘤有较好的疗效，且毒副作用可耐受，临床应用前景广阔。

项目成果

YOSHIKO KIMURA,S.NAKAMURA,A.AKAMUNE,S.TANADA,K.HAMAMOTO: "THE EFFECT OF VARIOUS CYTOKINES ON THE RADIOIMMUNOTHERAPY IN TUMOR-BEARING ATHYMIC MICE" J.NUCL.MED. 36-5. 216-216 (1995)

YOSHIKO KIMURA、S.NAKAMURA、A.AKAMUNE、S.TANADA、K.HAMAMOTO：“各种细胞因子对荷瘤无胸腺小鼠放射免疫治疗的影响”J.NUCL.MED。

(濱本 研) Ken HAMAMOTO: "Suppression of Liver Uptake of ^<111>In-Labeled Monoclonal Antibody in Radioimmunoscintigraphy" Eur.J.Nucl.Med.21. 759-759 (1994)

(Ken Hamamoto) Ken HAMAMOTO：“放射免疫闪烁显像中^<111>标记单克隆抗体的肝脏摄取的抑制”Eur.J.Nucl.Med.21 (1994)。

(木村良子) Yoshiko KIMURA: "The effect of various cytokines on the radioimmunotherapy in tumor bearing athymic mice" J.Nucl.Med.36. 216-216 (1995)

(Yoshiko Kimura) Yoshiko KIMURA：“各种细胞因子对荷瘤无胸腺小鼠放射免疫治疗的影响”J.Nucl.Med.36 (1995)。

KEN HAMAMOTO,Y.KIMURA,T.FUJII,A.AKAMUME,S.TANADA: "SUPPRESSION OF LIVER UPTAKE OF IN-111-LABELED MONOCLONAL ANTIBODY IN RADIOIMMUNOSCINTIGRAPHY" EUR.J.NUCL.MED.21-8. 759-759 (1994)

KEN HAMAMOTO、Y.KIMURA、T.FUJII、A.AKAMUME、S.TANADA：“放射免疫闪烁显像中对 IN-111 标记的单克隆抗体的肝脏摄取的抑制” EUR.J.NUCL.MED.21-8。

(木村良子) Yoshiko KIMURA: "Inhibition of the hepatic uptake of radiolabeled monoclonal antibody by various reagents in a primary rat hepatocyte culture model." Proceeding of the 1st China-Japanese Nuclear Medicine Conference 1995-5 Beijing. 49-49 (1995)

（Yoshiko Kimura） Yoshiko KIMURA：“原代大鼠肝细胞培养模型中各种试剂对放射性标记单克隆抗体的肝脏摄取的抑制”，第一届中日核医学会议论文集，1995年5月，北京，49-49。