Specific active immunotherapy with anti-idiotype monoclonal antibody in lung cancer patients

抗独特型单克隆抗体特异性主动免疫治疗肺癌患者

• 批准号: 05454381
• 负责人: FUJISAWA Takehiko
• 金额: $ 4.35万
• 依托单位: CHIBA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454381/
• 关键词: LUNG CANCER; SURGERY; ANTI-IDIOTYPE ANTIBODY; IMMUNOTHERAPY; ADJIVANT THERAPY; CELLULAR IMMUNITY; HUMORAL IMMUNITY; ANTIGEN SPECIFICITY; 血管浸潤; リンパ管浸潤; 免疫能; 腫瘍; モノクローナル抗体

Internal image anti-idiotype antibodies are expected to enhance anti-cancer effector mechanisms in vivo. We established hybridomas producing anti-idiotype monoclonal antibodies against a human monoclonal antibody (HuMoAb) 4G12 that reacts strongly with lung squamous cell carcinomas. Two clones reacted with 4G12 HuMoAb, but not with 3H12 IgM HuMoAb, human IgM,human serum or fetal calf serum. These two Ab2 antibodies (IgG1 kappa), 2B12 and 2H1 demonstrated 91.5% and 90.3% inhibition in their reactivity with radiolabelled 4G12 on PC10 cells, indicating that 2B12 and 2H1 antibodies were of Ab2 beta type. In crisscross inhibition assay, the binding of 2B12 or 2H1 to 4G12 was not inhibited by 2H1 or 2B12. Thus 2B12 and 2H1 were thought to recognize the different epitopes on the antigen binding sites. Antisera against 2B12 and 2H1 demonstrated specific reactivity to PC10 cells, confirming that 2B12 and 2H1 express internal images of lung squamous cell carcinoma recognized by the 4G12 antibody.

预期内部影像抗独特型抗体可增强体内抗癌效应机制。我们建立了杂交瘤生产抗独特型单克隆抗体对人单克隆抗体（人单克隆抗体）4G12，强烈反应与肺鳞状细胞癌。两个克隆与4G12 HuMoAb反应，但不与3H12 IgM HuMoAb、人IgM、人血清或胎牛血清反应。这两种Ab2抗体（IgG1 κ）2B12和2H1与放射性标记的4G12在PC 10细胞上的反应性显示出91.5%和90.3%的抑制，表明2B12和2H1抗体为Ab2 β型。交叉抑制实验中，2B12和2H1与4G12的结合不受2H1和2B12的抑制。因此，认为2B12和2H1识别抗原结合位点上的不同表位。针对2B12和2H1的抗血清显示出对PC10细胞的特异性反应性，证实2B12和2H1表达被4G12抗体识别的肺鳞状细胞癌的内部图像。

项目成果

Takehiko Fujisawa, et al.: "Strategy of diagnosis and treatment of lung cancer." Medical Practice. 11-5. 820-827 (1994)

Takehiko Fujisawa 等人：“肺癌的诊断和治疗策略。”

藤澤武彦: "抗イディオタイプモノクローナル抗体による肺非小細胞癌の診断と治療に関する検討" 病理生理. 14. 195-160 (1995)

Takehiko Fujisawa：“使用抗独特型单克隆抗体诊断和治疗非小细胞肺癌的研究”病理生理学 14. 195-160 (1995)。

藤澤武彦,他: "肺癌の診断と治療の基本戦略-組織型病期の診断は治療、予後にどう関連するか" Medical Practice. 11. 820-827 (1994)

Takehiko Fujisawa 等人：“肺癌诊断和治疗的基本策略 - 组织学分期的诊断与治疗和预后有何关系”《医学实践》11. 820-827 (1994)。

Takehiko Fujisawa: "Diagnosis and treatment of non-small cell carcinomas using anti-idiotype monoclonal antibody" Biopathology. 14-2. 159-160 (1995)

Takehiko Fujisawa：“使用抗独特型单克隆抗体诊断和治疗非小细胞癌”生物病理学。

藤澤武彦: "肺癌の診断と治療の基本戦略" Medical Practice. 11. 820-827 (1994)

Takehiko Fujisawa：“肺癌诊断和治疗的基本策略”医学实践 11. 820-827 (1994)。