Development of experimental myopia using a primate model (tree shrews) and the investigation for the mechanisms of myopia.

为了研究树鼩眼睛的变化，我们研究了测量（1）眼轴长度，（2）屈光不正，（3）眼部荧光的方法，以及新的计算机模拟方法来估计血视网膜屏障（BRB）。此外，还通过眼底检查和血管造影对正常树鼩的眼底特征进行了研究。对树？眼的正常发育过程进行了研究，以确定结构和透镜自发荧光的变化。在正常树鼩中，我们发现随着年龄的增长，角膜曲率增加，透镜增厚，玻璃体长度减少。这些变化在出生后100天迅速发生，然后在250天后缓慢发生。透镜自发荧光的程度随年龄增长而增加。我们还研究了一种诱导形觉剥夺的方法。在未成熟的树鼩中，从诱导形觉剥夺后的第二天开始，近视变化明显，玻璃体腔延长。这两种变化在诱导形觉剥夺后15天达到显著水平。这种趋势发生变化时，动物达到成熟，在成熟的树？的眼睛中，形觉剥夺诱导并没有显示在同一时期的近视变化。这表明成熟动物对形觉剥夺性近视的易感性可能会下降。透镜自发荧光值和BRB的内向渗透性增加后45天形觉剥夺诱导。然而，水的值，血-房水屏障的指数，没有改变。这些结果表明，近视的变化可能密切相关的视网膜功能受损的基础上BRB的渗透性与形觉剥夺性近视树鼩。此外，同时增加的透镜自体荧光可指示由近视发展引起的眼内环境稳定损害。

To investigate changes in tree shrew eyes, we studied the methodology for measuring (1) axial length, (2) refractive error, (3) ocular fluorescence, and the new computer simulation method to estimate the blood-retinal barrier (BRB). In addition, the fundus features of normal tree shrews were studied by fundus examination and angiography. The normal ocular development in tree shrews was studied to determine the structural and lens autofluorescence changes. In normal tree shrews, we found that with aging the corneal curvature increased, the lens thickened, and the length of the vitreous decreased. These changes occurred rapidly by 100 days after birth, then occurred slowly after 250 days. The degree of lens autofluorescence increased with age. We also investigated a method to induce form deprivation. In immature tree shrews, beginning the day after form deprivation was induced myopic changes were apparent and the vitreous cavity elongated. Both of these changes reached significant levels at 15 days following induction of form deprivation. This tendency changed when the animal reached maturity ; in mature tree shrews the eyes in which form deprivation was induced did not show the myopic changes during the same period. This suggests that susceptibility to form deprivation myopia may decline in mature animals. Lens autofluorescence values and the inward permeability of the BRB increased by 45 days after form deprivation was induced. However, the aqueous values, the indices of the blood-aqueous barrier, did not change. These findings indicate that myopic changes may be closely related to impaired retinal function based on the BRB permeability in tree shrews with form deprivation myopia. Furthermore, increased lens autofluorescence at the same time may indicated ocular homeostatic impairment caused by the development of myopia.