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Immunological and nutritional effects growth factor in small bowel transplantation

生长因子在小肠移植中的免疫和营养作用

基本信息

批准号：
05454484
负责人：
HAMADA Yoshinori
金额：
$ 4.29万
依托单位：
Kansai Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

Small animals such as rats are often used for the experiment model ofsmall bowel transplantation since they are easy handling and their strain is shown clearly. However, we have spent lots of times to establish the surgical procedure of transplant model in rats. A short segment of intestinal graft is favorable for reducing rejection, but less favorable for absorption of nutrients. We report that epidermal growth factor (EGF) augments the intestinal adaptation after small bowel transplanatation in rats.EGF has been reported to enhance adaptation in damaged intestines following massive intestinal resection. Studies were performed to determine whether EGF influences the recovery of intestinal function after small bowel transplanation in rats. Recipient Lewis rats underwent resection of the distal 80% of the small bowel, which was replaced with a 20 cm isograft. EGF (30mug/kg/day) or its vehicle was infused intraperitoneally for 3days after transplantation. After 7days, the graft was isolated for morphologic studies and was used for analysis of glucose and water absorption and the expression of sodium glucose contransporter1 (SGLT1). These were used as indicators of functional adaptation.The EGF-treated group exhibited significantly incteased mucosal villous height crypt cell proliferation glucose and water absorption, and expression of SGLT1 protein. No significant differences were found in body weight change or crypt depth between the two groups. These results demonstrate that EGF augments structural and functional adaptation of intestinal grafts in rats. EGF may be useful after intestinal transplanatation in patients with short bowel syndrome.

小肠移植的实验模型常采用大鼠等小动物，因为它们易于操作，且能清楚地显示其品系。然而，我们已经花费了大量的时间来建立大鼠移植模型的外科手术。移植肠段短有利于减少排斥反应，但不利于营养物质的吸收。我们报告表皮生长因子（EGF）增强大鼠小肠移植后的肠道适应性。EGF已被报道可增强大面积小肠切除后受损肠道的适应性。本研究旨在探讨表皮生长因子（EGF）对大鼠小肠移植后肠功能恢复的影响。将刘易斯大鼠的远端80%小肠切除，并用20 cm同种移植物替代。移植后腹腔内注入EGF（30 μ g/kg/d）或其赋形剂3d。7天后，分离移植物进行形态学研究，并用于分析葡萄糖和水的吸收以及钠葡萄糖转运蛋白1（SGLT 1）的表达。EGF治疗组大鼠胃粘膜绒毛高度、隐窝细胞增殖、葡萄糖和水的吸收以及SGLT 1蛋白的表达均显著增加。两组间体重变化和隐窝深度无显著差异。这些结果表明，EGF增强大鼠移植肠的结构和功能适应。EGF在短肠综合征患者的肠移植后可能是有用的。