Enhancement of intestinal adaptation by epidermal growth factor in the rat small bowel transplantation

表皮生长因子增强大鼠小肠移植的肠道适应

• 批准号: 12671184
• 负责人: HAMADA Yoshinori
• 金额: $ 2.5万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671184/
• 关键词: small bowel transplantation; epidermal growth factor; SGLT1; small bowel translantation; nitric oxide; EGF; 小腸移植; IEC6; 組織培養

Previous reports indicated that epidermal growth factor (EGF) has crucial role for graft adaptation in the rat small bowel transplantation. However, a little is known EGF influences various biological functions in intestinal graft except glucose absorption.The transplanted animals were treated with EGF or saline for one week. Effects of EGF on activities of disaccharidase and the induction of dipeptide cotransporter were investigated in addition to glucose absorption and intestinal histology.Levels of villous height and crypt depth in the EGF-treated group were significantly higher than those in the EGF-untreated group. EGF markedly increased glucose absorption and the induction of sodium glucose cotransporter (SGLT1) in the transplanted animals. EGF also stimulated the activities of disaccharidase such as maltase and sucrase, but it had no effect on the induction of sucrase-isomaltase. Further, EGF enhanced the induction of dipeptide cotransporter. These results demonstrate that EGF accelerates the recovery of mucosal structure and function, and intestinal allograft adaptation after small bowel transplantation.

已有报道表明，在大鼠小肠移植中，表皮生长因子(EGF)在移植物适应中起着重要作用。然而，除了葡萄糖吸收外，EGF对移植肠的各种生物学功能的影响知之甚少。移植动物接受EGF或生理盐水治疗一周。除葡萄糖吸收和肠道组织学检查外，还观察了EGF对二糖酶活性和二肽转运体诱导的影响。EGF处理组的绒毛高度和隐窝深度显著高于EGF未处理组。EGF显著增加移植动物对葡萄糖的吸收和钠葡萄糖共转运体(SGLT1)的诱导。EGF还能促进麦芽糖酶和蔗糖酶等二糖酶的活性，但对蔗糖酶-异麦芽糖酶的诱导作用不明显。此外，EGF增强了二肽共转运体的诱导作用。以上结果表明，EGF可促进小肠移植后肠粘膜结构和功能的恢复，促进移植肠的适应性。

项目成果

Kato Y, Hamada Y et al: "Epidermal growth factor enhances the intestinal adaptation of small bowel allografts in the postoperative period"Transplantation Proceedings. 32. 1253-1254 (2000)

Kato Y、Hamada Y 等人：“表皮生长因子增强小肠同种异体移植术后的肠道适应”移植论文集。

Nakai K, Hamada Y et al.: "Reciprocal induction of dipeptide and glucose co-transporters after all ogeneic graft small bowel transplantation."Transplantation Proceedings. 34・3. 1017-1018 (2002)

Nakai K、Hamada Y 等人：“所有异体移植小肠移植后二肽和葡萄糖协同转运蛋白的相互诱导”，34・3（2002 年）。

Kato Y, Hamada Y et al: "Epidermal growth factor stimulates intestinal adaptation of allogeneic graft after small bowel transplantation"Life Science. 70. 809-819 (2002)

Kato Y、Hamada Y 等人：“表皮生长因子刺激小肠移植后同种异体移植物的肠道适应”生命科学。

浜田吉則 他: "栄養-評価と治療"メジカルレビュー社. 5 (2002)

Yoshinori Hamada 等人：“营养-评估和治疗”医学评论出版 5 (2002)。

Nakai K, Hamada Y et al.: "Cycolprodigiosin hydrochloride as an immunosuppressant on rat small bowel transplantation"Transplantation Proceedings. 32. 1324-1325 (2000)

Nakai K、Hamada Y 等人：“盐酸环菌红作为大鼠小肠移植的免疫抑制剂”移植论文集。