Periodontal Ligament as a sensory apparaus : A morphological approach to functional property of periodontal nerve terminals

牙周韧带作为感觉装置：牙周神经末梢功能特性的形态学方法

• 批准号: 05454488
• 负责人: TAKANO Yoshiro
• 金额: $ 4.16万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454488/
• 关键词: periodontal ligament; PGP 9.5; Ruffini ending; development; nerve injury; histochemistry; immunohistochemistry; serotonin; Periodontal ligamert; Ruffini ending; development; nerve injury; histochenistry; immunohistocheristry; serotonvn; チロク

1. Double staining with non-specific cholinesterase and protein gene product 9.5 (PGP) clearly demonstrated that mechanoreceptors are restricted to the area near root apex while nociceptors are densely distributed throughout the whole length of periodontal ligament.2. In histochemical studies, almost all mitochondria in the axon terminals of Ruffini endings showed intense non-specific cholinesterase. Terminal Scwann cells associated with periodontal Ruffini endings were positive for acid phosphatase.3. The development and maturation process of Ruffini endings were investigated by use of immunohistocheistry and immunocytochemistry for PGP.These processed require the additions of functional stimuli such as tooth eruption and occlusal force.4. The responses of periodontal nerves to experimentally induced occlusal trauma in rat molars were assessed by immunohistochemistry for PGP.By the second day after bite-raising, many Ruffini endings were swollen and their outline were unclear at light … More microscopic level. Under electron microscope, unusual long cytoplasmic projections extended through enlarged slits of Schwann cell covering, and the cavcolac of the Schwann sheaths had decreased in number. Furthermore, from days 2 to 4, thin nerve fibers on the pressure side of the periodontal ligament were oriented irregulary and had a prominet beated appearance. An increase in the beaded nerves occurred in between days 2-4 post elevation, and decreased over the time course. These results suggest celusal trauma induces specific changes in the distribution and shape of nerve terminals in the periodontal ligament.5. For elucidation in the kinds of neuromodulater concerning jaw-reflexes, microinjection with HRP into masseter was done followed by immunocytochemistry for serotonin in cat trigeminal motor nucleus. The HRP-labelled motor neuron formed synapse with serotonin-positive nerve fibers. The results in this study suggest serotonin is one of neuromodulators for control of jaw-movement. Less

1.非特异性胆碱酯酶和蛋白基因产物9.5（PGP）双染显示，机械感受器仅分布于根尖附近，而伤害感受器密集分布于牙周膜全长.在组织化学研究中，几乎所有的线粒体在轴突终末的Ruffini表现出强烈的非特异性胆碱酯酶。与牙周Ruffini终末相关的终末雪旺细胞酸性磷酸酶阳性.利用细胞化学和免疫细胞化学方法研究了Ruffini终末的发育和成熟过程，这些过程需要牙齿萌出和咬合力等功能刺激的参与.用免疫组织化学方法观察大鼠磨牙咬合创伤后牙周神经的反应，结果发现，咬合创伤后第2天，许多Ruffini神经末梢肿胀，在光照下轮廓不清 ...更多信息 微观水平。电镜下可见异常长的胞质突起穿过扩大的雪旺氏细胞覆盖物的缝隙，雪旺氏鞘的腔室数量减少。第2 ~ 4天，牙周膜压力侧的神经纤维排列不规则，呈锯齿状。串珠神经的增加发生在海拔后2-4天之间，并随着时间的推移而减少。这些结果提示，牙周膜创伤后，牙周膜内神经末梢的分布和形态发生了特异性改变.为阐明与颌反射有关的神经调节物质的种类，本实验采用咬肌内注射HRP，并对猫三叉神经运动核内5-羟色胺进行免疫细胞化学染色。HRP标记的运动神经元与神经纤维形成突触。本研究结果提示5-羟色胺是控制下颌运动的神经调质之一。少

项目成果

Kawahara I.et al.: "Immunohistochemical characterization and Iocalization of MHC class II antigen presenting cells in the periodontal ligament of rat incisors." Connect.Tissue Res.(in press). (1995)

Kawahara I.et al.：“大鼠门牙牙周韧带中 MHC II 类抗原呈递细胞的免疫组织化学特征和定位。”

T.Maeda,T.Sodeyama,T.Hara,Y.Takano: "Evidence for the existence of intraepithelial nerve endings in the junctionaol epithelium of rat molars:an immunohistochemical study using protein gene product9./5(PGP9.5)antibody." Journal of Periodontal Research. 29.

T.Maeda、T.Sodeyama、T.Hara、Y.Takano：“大鼠磨牙交界上皮中存在上皮内神经末梢的证据：使用蛋白质基因产物9./5(PGP9.5)抗体进行的免疫组织化学研究。

T.Maeda,S.Homma,Y.Takano: "Dense innervation of human radicular dental lpulp as revealed by immunocytochemistry for protein qene product9.5." Archives of Oral Biology. 39. 563-568 (1994)

T.Maeda、S.Homma、Y.Takano：“通过蛋白质 qene 产物 9.5 的免疫细胞化学揭示人根牙髓的密集神经支配。”

T.Maeda et al.: "Histochemical demonstration of acid phosphatase activity in periodontal Ruffini endings in rat incisors." Arch.Oral Biol.38. 611-617 (1993)

T.Maeda 等人：“大鼠门牙牙周 Ruffini 末端酸性磷酸酶活性的组织化学证明。”

Maeda t. et al.: "Histochemical demonstration of acid phosphatase activity in periodontal Ruffini endigs in rat incisor." Archs.Oral Biol.38. 611-617 (1993)

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