Immunohistochemical and Enzyme-histochemical studies on the cellular networks of antigen-presenting cells in the dental and periodontal tissues.

对牙齿和牙周组织中抗原呈递细胞的细胞网络进行免疫组织化学和酶组织化学研究。

• 批准号: 07457426
• 负责人: TAKANO Yoshiro
• 金额: $ 4.16万
• 依托单位: TOKYO MEDICAL AND DENTAL UNIVERSITY (1996)Niigata University (1995)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457426/
• 关键词: antigen-presenting cell; dendritic cell; macrophage; MHC class II; immunohistochemistry; osteoclast; periodontal ligament; dental pulp; MHC Class II; 酸性フォスファターゼ

In order to elucidate the entire biological network of antigen-pressenting cells in the oral regions, incisors and molar tooth germs of the rat and human permanent as well as decidous teeth were examined with respect to the following aspects ;1. Antigen-presenting cell network : Cellular networks of the antigen-presenting cells including macrophages were examined by neans of the double staining method with immunostaining for and anti-MHC class II antigen and enzyme histochemistry for ACPase. Dendritic cells (DC) and macrophages were discriminated in both the dental pulp and periodontal ligament (PDL), and their localization patterns demonstrated. In young animals, the dendritic cells were small in number and gradually increased as they grew. In the PDL of rat molars, the DC and osteoclasts showed distinct segregated localization. In the pulp tissues of rat and human teeth, the DC often extended long cell processes deep into the dentinal canals under physiological conditions.2. Cavity p … More reparation and dendritic cells : Early responses of DC in the dental pulp to cavity preparation was clearly demonstrated for the first time. The DC were shown to accumulate at the traumatic regions of the odontoblastic layr immediately after cavity preparaton. They remained there until the onset of the dposition of the reparative dentin. A possible role of the DC in pulp repair in addition to reception and processing of foreign antigens was suggested.3. Discrimination between the precursor cells of DC and osteoclasts : In the PDL of rat molars where physiological bone resorption proceeds at the distal face of the alveolar bone, DC predominantly located at the proximal PDL where bone formation was dominant. Orthodontic force from distal to proximal direction of the molar tooth caused different spatio-temporal arrangement of the DC in the PDL.In this experiment model, the precursor cells of the DC and osteoclasts were surveyed.4. DC in human deciduous teeth : The distribution and cytological features of DC in human deciduous teeth at different functional stages (normal, root resorption, crown resorption) were examined. The DC in the deciduous teeth often inserted cytoplasmic processes into the dentinal canals as was the case in permanent teeth. A functional correlation of the pulpal DC with cementogenesis that occasionally commenced after resorption of the crown dentin was suggested. Less

为了阐明口腔区域抗原提呈细胞的整个生物网络，对大鼠和人恒牙以及乳牙的切牙和磨牙牙胚进行了以下方面的检查：1.抗原提呈细胞网络：通过免疫染色和抗MHC II类抗原的双重染色法以及ACEI的酶组织化学法检查抗原提呈细胞（包括巨噬细胞）的细胞网络。在牙髓和牙周膜（PDL）中可以识别出树突状细胞（DC）和巨噬细胞，并展示了它们的定位模式。在年幼的动物中，树突状细胞的数量很少，并且随着它们的生长而逐渐增加。在大鼠磨牙牙周膜中，DC和破骨细胞呈明显的分离定位。在大鼠和人的牙髓组织中，DC在生理条件下常向牙本质管内延伸较长的细胞突起.腔p ...更多信息 修复和树突状细胞：第一次清楚地证明了牙髓中DC对洞制备的早期反应。结果显示，成牙本质细胞层损伤后，DC在损伤区大量聚集。它们一直保持在那里，直到修复性牙本质沉积的开始。DC除了接受和处理外源性抗原外，还可能在牙髓修复中发挥作用. DC前体细胞与破骨细胞的区别：在大鼠磨牙的PDL中，生理性骨吸收在牙槽骨的远端面进行，DC主要位于近端PDL，其中骨形成占主导地位。从磨牙远中向近中方向施加正畸力，导致牙周膜内DC的时空分布不同，在本实验模型中，对DC的前体细胞和破骨细胞进行了观察.人乳牙中的树突状细胞：观察正常人乳牙、牙根吸收人乳牙、牙冠吸收人乳牙中树突状细胞的分布及细胞学特征。与恒牙一样，乳牙中的树突状细胞常以胞质突起插入牙本质管内。牙髓DC与牙骨质形成的功能相关性，偶尔开始后，冠牙本质的吸收建议。少

项目成果

花泉 好訓・河野 芳朗 他1名: "イヌのハンターシュレ-ゲル条の三次元的観察" エナメル質比較発生学懇話会記録. 4. 31-44 (1995)

Yoshinori Hanaizumi、Yoshiro Kono 等 1：“狗亨特施莱格尔条纹的三维观察”牙釉质比较胚胎学会议记录 4. 31-44 (1995)。

Kawahara, I.and Takano Y.: "Segregated localization of immunocompetent cells and osteoclasts in the periodontal ligament of rat molar tooth." Arch.Histo.Cytol.58 (3). 345-355 (1995)

Kawahara, I. 和 Takano Y.：“大鼠磨牙牙周韧带中免疫活性细胞和破骨细胞的分离定位。”

Takano, Y., Kawahara, I., Hoshino, M., Takeuchi, K., Maeda, T., Ohshima, H., Hanaizumi, Y.and Kawano, Y.: "Dendritic Cells : A Novel Cellular Component of the Rat incisor Enamel Organ in the Late Stages of Enamel Maturation." Adv.Dent.Res.10 (2). 216-224

Takano, Y.、Kawahara, I.、Hoshino, M.、Takeuchi, K.、Maeda, T.、Ohshima, H.、Hanaizumi, Y. 和 Kawano, Y.：“树突状细胞：树突状细胞的一种新型细胞成分

Ohtsu, A., Kusakari, H., Maeda, T.and Takano, Y.: "A histological investigation on tissue responses to titanium-implantation in cortical bone of the rat femur." J.Periodontol.(in press). (1996)

Ohtsu, A.、Kusakari, H.、Maeda, T. 和 Takano, Y.：“对大鼠股骨皮质骨钛植入的组织反应的组织学研究。”

Kawahara,I.Takano,Y: "Segregated Iocalization of immunocompetent cells and osteoclasts in the periodontal ligament・・・・" Arch. Histol. Cytol. 58(3). 345-355 (1995)

Kawahara，I. Takano，Y：“牙周韧带中免疫活性细胞和破骨细胞的分离定位......”Histol。58（3）（1995）。