A Bio-Engineering Research on the Sensing and Responding Mechanism of Vascular Endothelial Cells to Blood Flow
血管内皮细胞对血流感知与响应机制的生物工程研究
基本信息
- 批准号:05454692
- 负责人:
- 金额:$ 4.42万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1993
- 资助国家:日本
- 起止时间:1993 至 1995
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The results of our present study consists of two major findings. One is that a signal transduction mechanism by which vascular endothelial cells perceive blood flow and transmit the signal into the interior of the cell via calcium ions has been demonstrated. The other one is that endothelial cells alters their expression of adhesion molecules at both mRNA and surface protein levels in response to changes in blood flow. In regard to the flow sensing mechanism, we observed that cultured bovine endothelial cells showed an elevation in intracellular calcium ion concentration when exposed to shear stress in a flow-loading apparatus. The elevation of calcium consentration was shear-stress dependent in the presence of extracellular ATP,which had nothing to do with membrane potential and stretch-activated ion channels, and the main source for the calcium response was an influx of extracellular calcium ions. These findings indicate that calcium ions may play a role in a signal transduction of blood flow by vascular endothelial cells. As for the cell response to blood flow, we demonstrated that fluid shear stress down-regulates the cell surface expression of vascular cell adhesion molecule-1 (VCAM-1) in cultured mouse endothelial cells. The decrease in VCAM-1 expression was due to a suppression of VCAM-1 mRNA induced by shear stress. Thus, blood flow seems to act as a regulator for adhesion molecule expression in endothelial cells.
我们目前的研究结果包括两个主要发现。一个是已经证明了血管内皮细胞感知血流并通过钙离子将信号传递到细胞内部的信号转导机制。另一个是内皮细胞在mRNA和表面蛋白水平上改变其粘附分子的表达以响应血流的变化。在流动传感机制方面,我们观察到,培养的牛内皮细胞在细胞内钙离子浓度升高时,暴露于剪切应力在流动加载装置。在细胞外ATP存在的情况下,钙离子浓度的升高是剪切应力依赖性的,与膜电位和牵张激活离子通道无关,钙离子反应的主要来源是细胞外钙离子的内流。这些发现表明,钙离子可能在血管内皮细胞的血流信号转导中起作用。至于细胞对血流的反应,我们证明了流体切应力下调培养的小鼠内皮细胞中血管细胞粘附分子-1(VCAM-1)的细胞表面表达。VCAM-1表达的减少是由于剪切应力诱导的VCAM-1 mRNA的抑制。因此,血流似乎作为内皮细胞中粘附分子表达的调节剂。
项目成果
期刊论文数量(76)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
J.Ando: "Flow-induced calcium transients and release of endothelium-derived relaxing factor in cultured vascular endothelial cells" Frontiers Med.Biol.Engng.5. 17-21 (1993)
J.Ando:“培养的血管内皮细胞中流动诱导的钙瞬变和内皮衍生舒张因子的释放”Frontiers Med.Biol.Engng.5。
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R.Korenaga: "Laminar flow stimulates ATP- and shear stress-dependent nitric oxide production in cultured bovine endothelial cells." Biochem.Biophys.Res.Commun.198. 213-219 (1994)
R.Korenaga:“层流刺激培养的牛内皮细胞中依赖于 ATP 和剪切应力的一氧化氮的产生。”
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- 影响因子:0
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A. Kamiya: "Endothelial cell responses to fluid shear stress. In “Endothelium-derivved factors and vascular functions"," Elsevier Science Amsterdam T. Masaki ed.,10 (1994)
A. Kamiya:“内皮细胞对流体剪切应力的反应。在“内皮衍生因子和血管功能”中,”爱思唯尔科学阿姆斯特丹 T. Masaki 编辑,10 (1994)
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J.Ando: "Shear stress inhibits the adhesion of cultured mouse endothelial cells to lymphocytes by down-regulation VCAM-1 expression." Am.J.Physiol. 267. C679-C687 (1994)
J.Ando:“剪切应力通过下调 VCAM-1 表达来抑制培养的小鼠内皮细胞与淋巴细胞的粘附。”
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J.Ando: "The regulation of coronary blood flow" Springer-Verlag Tokyo, 327 (1991)
J.Ando:“冠状动脉血流的调节”Springer-Verlag Tokyo,327(1991)
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{{ truncateString('ANDO Joji', 18)}}的其他基金
Molecular Biomechanics of Vascular Cell Mechano-Responses
血管细胞机械反应的分子生物力学
- 批准号:
21220011 - 财政年份:2009
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (S)
Ion channel-mediated blood flow-sensing mechanism in vascular endothelial cells
血管内皮细胞离子通道介导的血流传感机制
- 批准号:
18200030 - 财政年份:2006
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Biomechanical force-sensing and response mechanisms in vascular cells and its application to tissue engineering
血管细胞的生物力学力传感和响应机制及其在组织工程中的应用
- 批准号:
17076002 - 财政年份:2005
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Mechano-sensing Mechanism and Mechano-Responsive Genes in Vascular Endothelial Cells
血管内皮细胞的机械感应机制和机械响应基因
- 批准号:
15200034 - 财政年份:2003
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Exploration of blood flow-sensing molecules in vascular endothelial cells
血管内皮细胞血流传感分子的探索
- 批准号:
13308052 - 财政年份:2001
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Elucidation of the mechanism of vascular cell sensing and gene responses to mechanical stresses
阐明血管细胞感知和基因对机械应力的反应机制
- 批准号:
11308035 - 财政年份:1999
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (A).
Cloning of shear stress-sensitive endothelial genes
剪切应力敏感内皮基因的克隆
- 批准号:
08408039 - 财政年份:1996
- 资助金额:
$ 4.42万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
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