Intelligent Biosensor Based on the Enzyme Array Electrodes

基于酶阵列电极的智能生物传感器

• 批准号: 05640687
• 负责人: YAO Toshio
• 金额: $ 1.41万
• 依托单位: University of Osaka Prefecture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05640687/
• 关键词: Biosensor; Intelligent sensor; Microsensor; Multifunctional sensor; Microdialysis sensor; ミクロ酵素電極; インテリジェントバイオセンサー; アレイ酵素センサー

1. The Silica gel film containing enzymes was formed on the array platinum chip coated with poly (o-phenylenediamine) thin film, by a sol-gel method from the mixed solution composed of tetramethyl orthosilicate and enzymes. This sensor responded simultaneously, rapidly and specificically to a variety of substrates.2. A direct electron transfer between graphite and adsorbed peroxidase was found to take place effectively, to continue the catalytic cycle of the enzymes. This sensor responded to hydrogen peroxide without any electron mediator. This sensing method was applied to the preparation of the various enzyme sensors, by coadsorbing oxidase and peroxidase. Furthermore, enzyme array sensors prepared by immobilizing enzymes into the Os redox polymer was found to posess a molecular wire function based on the direct electron transfer between enzyme and electrode.3. The sensors for taste and freshness were prepared according to the procedures described above and their functions were evaluated using a variety of samples.4. A new microdialysis sensor, which includes a microdialysis probe and immobilized enzyme reactor, could be successfully applied to in vivo monitoring of biologically important substrates in rat brain.

1.以正硅酸四甲酯和酶的混合溶液为原料，采用溶胶-凝胶法在聚邻苯二胺薄膜涂层的阵列铂芯片上制备了含酶的硅胶薄膜。该传感器对多种底物具有同时、快速、特异性的响应.石墨和吸附的过氧化物酶之间的直接电子转移被发现有效地发生，继续酶的催化循环。该传感器响应过氧化氢没有任何电子介体。该传感方法适用于各种酶传感器的制备，通过共吸附氧化酶和过氧化物酶。此外，将酶固定在Os氧化还原聚合物上制备的酶阵列传感器，基于酶与电极之间的直接电子转移，具有分子导线功能.根据上述程序制备用于味道和新鲜度的传感器，并使用各种样品评价它们的功能。一种新型的微透析传感器，它包括一个微透析探针和固定化酶反应器，可成功地应用于在体监测的生物重要底物在大鼠脑。

项目成果

T.Yao: "Enzyme Electrode for the Successive Detection of Hypoxanthine and Inosine" Anal.Chim.Acta. 281. 323-326 (1993)

T.Yao：“用于连续检测次黄嘌呤和肌苷的酶电极”Anal.Chim.Acta。

T.Yao: "Simultaneous Determination of Sulfite and Phosphate in Wine by Means of Immobilized Enzyme Reactions and Amperometric Detection in a Flow-Injection System" Talanta. 41. 2113-2119 (1994)

T.Yao：“通过流动注射系统中的固定化酶反应和电流检测同时测定葡萄酒中的亚硫酸盐和磷酸盐”Talanta。

ToshioYao: "An Enzyme Electrode for the Suc cessive Detection of Hypoxanthine and Inosine" Analytica Chimica Acta. 281. 323-326 (1993)

ToshioYao：“用于成功检测次黄嘌呤和肌苷的酶电极”《分析化学学报》。

T.Yao: "Amperometric Detection of Purine Nucleotides Utilizing Immobilized Enzyme Reactors and On-line Amplification by Substrate Recycling" Electroanalysis. 6. 165-169 (1994)

T.Yao：“利用固定化酶反应器和底物回收在线扩增对嘌呤核苷酸进行安培检测”电分析。

八尾俊男: "ペルオキシダーゼ含有グラファイト電極における酵素-電極間直接電子移動と酵素電極への応用" 分析化学. 42. 411-415 (1993)

Toshio Yao：“含过氧化物酶的石墨电极中酶与电极之间的直接电子转移及其在酶电极中的应用”《分析化学》42. 411-415 (1993)。