Intelligent Biosensor Based on the Enzyme Array Electrodes

1. 以正硅酸四甲基与酶的混合溶液为原料，采用溶胶-凝胶法在包覆聚邻苯二胺薄膜的阵列铂芯片上形成了含酶的硅胶膜。这种传感器对各种衬底同时、迅速、特异地作出反应。石墨和吸附过氧化物酶之间的直接电子转移有效地发生，继续酶的催化循环。这种传感器在没有任何电子介质的情况下对过氧化氢有反应。将该传感方法应用于氧化酶和过氧化物酶的共吸附制备各种酶传感器。此外，将酶固定在Os氧化还原聚合物中制备的酶阵列传感器具有基于酶和电极之间直接电子转移的分子线功能。根据上述程序制备了味道和新鲜度传感器，并使用各种样品对其功能进行了评估。一种新型微透析传感器，包括微透析探针和固定化酶反应器，可以成功地应用于大鼠脑内重要生物底物的体内监测。

1. The Silica gel film containing enzymes was formed on the array platinum chip coated with poly (o-phenylenediamine) thin film, by a sol-gel method from the mixed solution composed of tetramethyl orthosilicate and enzymes. This sensor responded simultaneously, rapidly and specificically to a variety of substrates.2. A direct electron transfer between graphite and adsorbed peroxidase was found to take place effectively, to continue the catalytic cycle of the enzymes. This sensor responded to hydrogen peroxide without any electron mediator. This sensing method was applied to the preparation of the various enzyme sensors, by coadsorbing oxidase and peroxidase. Furthermore, enzyme array sensors prepared by immobilizing enzymes into the Os redox polymer was found to posess a molecular wire function based on the direct electron transfer between enzyme and electrode.3. The sensors for taste and freshness were prepared according to the procedures described above and their functions were evaluated using a variety of samples.4. A new microdialysis sensor, which includes a microdialysis probe and immobilized enzyme reactor, could be successfully applied to in vivo monitoring of biologically important substrates in rat brain.