Intelligent Biosensor Based on the Enzyme Array Electrodes

1.以正硅酸四甲酯和酶的混合溶液为原料，采用溶胶-凝胶法在聚邻苯二胺薄膜涂层的阵列铂芯片上制备了含酶的硅胶薄膜。该传感器对多种底物具有同时、快速、特异性的响应.石墨和吸附的过氧化物酶之间的直接电子转移被发现有效地发生，继续酶的催化循环。该传感器响应过氧化氢没有任何电子介体。该传感方法适用于各种酶传感器的制备，通过共吸附氧化酶和过氧化物酶。此外，将酶固定在Os氧化还原聚合物上制备的酶阵列传感器，基于酶与电极之间的直接电子转移，具有分子导线功能.根据上述程序制备用于味道和新鲜度的传感器，并使用各种样品评价它们的功能。一种新型的微透析传感器，它包括一个微透析探针和固定化酶反应器，可成功地应用于在体监测的生物重要底物在大鼠脑。

1. The Silica gel film containing enzymes was formed on the array platinum chip coated with poly (o-phenylenediamine) thin film, by a sol-gel method from the mixed solution composed of tetramethyl orthosilicate and enzymes. This sensor responded simultaneously, rapidly and specificically to a variety of substrates.2. A direct electron transfer between graphite and adsorbed peroxidase was found to take place effectively, to continue the catalytic cycle of the enzymes. This sensor responded to hydrogen peroxide without any electron mediator. This sensing method was applied to the preparation of the various enzyme sensors, by coadsorbing oxidase and peroxidase. Furthermore, enzyme array sensors prepared by immobilizing enzymes into the Os redox polymer was found to posess a molecular wire function based on the direct electron transfer between enzyme and electrode.3. The sensors for taste and freshness were prepared according to the procedures described above and their functions were evaluated using a variety of samples.4. A new microdialysis sensor, which includes a microdialysis probe and immobilized enzyme reactor, could be successfully applied to in vivo monitoring of biologically important substrates in rat brain.