Search for genes involved in plant tumor and teratoma formation

寻找参与植物肿瘤和畸胎瘤形成的基因

基本信息

批准号：
05640701
负责人：
WABIKO Hiroetsu
金额：
$ 1.54万
依托单位：
Biotechnology Institute Akita Prefectural College of Agriculture Ohgata, Akita 010-04
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

A soil bacterium Agrobacterium tumefaciens induces crown gall disease on many plants. Tumorous tissues display various phenotypes on the culture medium. We assumed that in these tissues, the genes involved in plant differentiation could be induced or repressed, and thus current project is directed to isolation and characterization of such genes. We first generated shoot-forming and unorganized calli in tobacco plants upon introduction of auxin and cytokinin biosynthesis genes, and 6b gene which modulates the effects of these plant growth substances. Complementary DNA was synthesized from these tissues and screened for differential expression by differential hybridization and differential display techniques. Five clones and four clones were found to be preferentially expressed in shoot-forming callus and unorganized callus respectively. The former clones were homologous to the genes encoding 14kD protein from carrot, SAR8.2 associated with tobacco mosaic virus infection, heat shock protein, chlorophyll a apoprotein psaB,and ribonucleoprotein from tobacco. The latter clones were similar to tobacco chitinase, ribosomal protein L29 from Arabidopsis, allergenic protein Lolpl from rye, and as yet unknown clone termed 1-22A.Particularly, the 14kD-protein gene has been reported to be associated with regeneration of carrot cells, and hence we cloned our homologue under the control of CaMV35S promoter in an antisense orientation, and introduced to tabacco. Unfortunately, resulting transgenic plants did not show any phenotypic abnormality. However, the gene expression was induced by wounding, which suggest that the 14kD-protein gene is involved in stress response. Finally, the psaB and ribonucleoprotein gene seem to be required for chloroplast development, and further investigation is necessary to determine the relationship between plastid and total plant development.

土壤细菌农杆菌tumefaciens在许多植物上诱导皇冠胆疾病。肿瘤组织在培养基上显示各种表型。我们假设在这些组织中，参与植物分化的基因可以被诱导或抑制，因此当前的项目针对这种基因的分离和表征。我们首先在引入生长素和细胞分裂素生物合成基因和6B基因后，首先在烟草植物中生成芽形成和无组织的Calli，这些基因调节了这些植物生长物质的作用。从这些组织中合成互补的DNA，并通过差异杂交和差异显示技术筛选出差异表达。发现五个克隆和四个克隆分别在形成的愈伤组织和无组织的愈伤组织中优先表达。前克隆与来自胡萝卜的14KD蛋白的基因同源，SAR8.2与烟草病毒感染相关，热休克蛋白，叶绿素apoprotein psab和来自烟草的核糖核蛋白。 The latter clones were similar to tobacco chitinase, ribosomal protein L29 from Arabidopsis, allergenic protein Lolpl from rye, and as yet unknown clone termed 1-22A.Particularly, the 14kD-protein gene has been reported to be associated with regeneration of carrot cells, and hence we cloned our homologue under the control of CaMV35S promoter in an antisense方向，并介绍给Tabacco。不幸的是，由此产生的转基因植物没有表现出任何表型异常。然而，基因表达是通过伤口诱导的，这表明14KD蛋白基因参与胁迫反应。最后，叶绿体发育似乎需要PSAB和核糖核蛋白基因，并且需要进一步研究以确定质体和总植物发育之间的关系。