权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Dynamics of plant hormones in plant tumor formation and cell division

植物激素在植物肿瘤形成和细胞分裂中的动态

基本信息

批准号：
15570043
负责人：
SYONO Kunihiko
金额：
$ 2.37万
依托单位：
Japan Women's University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570043/
关键词：
genetic tumor root nodule DR5-GUS auxin GA Lotus japonicus tobacco Lactuca sp.植物腫瘍同調分裂植物ホルモン

项目摘要

The relation between plant gall formation, such as root nodule, genetic tumor, crown gall and etc., and changes of the content of plant growth hormones has been analyzed mainly by bioassay and/or analytical instruments. However, these methods aren't enough to analyze minute aspects of special and temporal changes of plant hormones due to the restriction of sampling.In this project, in addition to these methods, the construct of plant-hormones-responsive promoter combined with GUS reporter gene, such as DR5-GUS for auxin, was used. The obtained major results are summarized below.1. DR5-GUS was introduced into tumor-proned tobacco F1 plant (Nicotiana glauca x N.langsdorffii). The analysis using the transformants showed the tumors will be induced by wound via increase of auxin content followed the promotion of expression of auxin-induced genes such as NgrolB.2. When the seeds of Lactuca sp. were germinated aseptically in vitro, tumors developed spontaneously at the site between hypocotyl … More and root. As analysis of the seedling of DR5-GUS transformants of Arabidopsis showed the accumulation of auxin at the similar site, transformation of Lactuca sp. by DR5-GUS was attempted. The obtained transformants will be useful for analysis of tumor formation processes.3. GC-MS analysis showed that the content of auxin increased at the early stage of root nodule development in Lotus japonicus. For further analysis of auxin function in the development of root nodule, DR5-GUS transformants were successfully isolated.4. GC-MS analysis showed that in the early stage of the development of, root nodules in Lotus japonicus, GA content increased remarkably. Inhibitors of GA biosynthesis inhibited the root nodule formation. However, the inhibition was recovered by GA. These results suggest that GA involved in root nodule formation at early stage of development.5. High activity of indole-3-acetamide hydrolase, which involve in auxin biosynthesis of crown gall, was detected in rice cells. The enzymatic activity was partially purified and the nature of enzyme was clarified.It was also found that the expression of NgORF13 in tobacco genetic tumor was promoted by wound. Jasmonate was shown to be involved in the promotion. Less

植物根瘤、遗传瘤、冠瘤等根瘤的形成与植物生长激素含量变化的关系主要通过生物测定和/或分析仪器来分析。然而，由于采样条件的限制，这些方法不足以分析植物激素的特殊和时间变化的细微方面，在本项目中，除了这些方法外，还使用了植物激素反应启动子和GUS报告基因相结合的构建方法，如生长素的DR5-GUS。所取得的主要成果总结如下：1.将DR5-GUS基因导入带瘤苗的烟草F1植株。利用转化子的分析表明，随着生长素诱导基因如NgrolB表达的促进，创伤将通过增加生长素含量来诱导肿瘤的发生。当莴苣属植物的种子。在体外无菌萌发，肿瘤在下胚轴…之间的位置自发生长更多的和根。对拟南芥DR5-GUS转化体幼苗的分析表明，生长素在类似的位置积累，转化生菜。由DR5-GUS尝试。获得的转化子可用于肿瘤形成过程的分析。GC-MS分析表明，在莲花根瘤发育的早期，生长素的含量增加。为进一步研究生长素在根瘤发育中的作用，成功分离到DR5-GUS转化子。GC-MS分析表明，在莲花根瘤发育的早期，GA含量显著增加。GA生物合成抑制剂抑制根瘤的形成。但GA可恢复这种抑制作用。这些结果表明赤霉素A参与了根瘤的早期发育。在水稻细胞中检测到高活性的吲哚-3-乙酰胺水解酶，该酶与冠瘤生长素的生物合成有关。对NgORF13的活性进行了部分纯化，明确了酶的性质，并发现NgORF13在烟草遗传肿瘤中的表达受到创伤的促进。茉莉酸被证明参与了促销活动。较少