Analysis of the viroid structural domains regulating viroid pathogenicity

调节类病毒致病性的类病毒结构域分析

• 批准号: 05660042
• 负责人: SANO Teruo
• 金额: $ 1.34万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660042/
• 关键词: citrus exocortis viroid; hop stunt viroid; viroid chimera; pathogenicity; structural domain; カンキツエクソコーテイスウイロイド; ウイロイド; CEVd; HSVd; TASVd; 低分子RNA

To Investigate the viroid structural domains regulating viroid pathogenicity, we have produced the recombinant cDNA clones pBS-CE/HS-TR,pBS-HS-CE-TR and pBS-CE/AS-TR by exchanging the terminal right (TR) domain amond HSVd, CEVd and TASVd. We focused our analysis mainly on the roles of the TR domains in the viroid pathogenisity.We have constructed the chimeric viroid cDNA clones described above using recombinant DNA techniques and PCR in the first year (1993). From the last quarters in the first year, we started the infectivity assays using the recombinant chimeric cDNA clones and found that,(1)the pBS-CE/HS-R and pBS-HS-CE-TR was infectious, and replicated stable in cucumber and tomoto,(2)but pBS-CE/AS-TR was nou infectious.Furthermore, we have accomplished the critical analysis on the pathogenicity of the infectious viroid chimeras (CE/HS-TR and HS/CE-TR), and revealed that TR domain was critical for the viroid replication or accumulation.Our results showed that,(1)the TR domain was exchangeable without loosing their viability between the different viroid group such as HSVd group and PSTVd group.(2)the TR domain regulates viroid pathogenicity via its replication and/or accumulation rates in the infected host plants.

为了研究类病毒结构域对类病毒致病性的调控作用，我们将HSVd、CEVd和TASVd的末端右（TR）结构域进行了互换，获得了重组cDNA克隆pBS-CE/HS-TR、pBS-HS-CE-TR和pBS-CE/AS-TR。我们主要分析了TR结构域在类病毒致病性中的作用，并在第一年（1993年）利用重组DNA技术和PCR技术构建了上述嵌合类病毒cDNA克隆。从第一年的最后一个季度开始，我们利用重组嵌合cDNA克隆进行了侵染性测定，发现：（1）pBS-CE/HS-R和pBS-HS-CE-TR具有侵染性，并且在黄瓜和番茄中稳定复制;（2）pBS-CE/AS-TR不具有侵染性，并对侵染性类病毒嵌合体的致病性进行了初步分析结果表明：（1）TR结构域在HSVd和PSTVd两组类病毒之间是可以互换的，但并不影响类病毒的生存能力;（2）TR结构域通过其在被感染的寄主植物中的复制和/或积累速率来调节类病毒的致病性。

项目成果

佐野輝男: "ウイロイド(Viroid)/hepatitis Sとの関連" ウイルス. 44. 27-34 (1994)

Teruo Sano：“类病毒/与 S 型肝炎的关系”病毒。 44. 27-34 (1994)

Teruo Sano: "Viroid/the relationship with hepatitis delta" Virus. Vol44. 27-34 (1994)

Teruo Sano：“类病毒/与丁型肝炎的关系”病毒。

佐野輝男: "ウイロイド(Viroid)/hepatitis δ との関連" ウイルス. 44. 27-34 (1994)

Teruo Sano：“类病毒/与 δ 型肝炎的关系”病毒 44. 27-34 (1994)。

佐野輝男: "ウイロイドの病原性発現機構の解析と弱毒化の可能性について" 平成5年度文部省特定研究「有用微生物資源の探索と生物環境制御」. 66-72 (1994)

佐野辉雄：《类病毒致病性表达机制分析及减毒可能性》1993年教育部专题研究《有用微生物资源探索与生物环境控制》66-72（1994）。

Teruo Sano: "Analysis on the mechanisms of viroid pathogenicity and its application for the viroid diseases control" Research Report, Tokutei-Kenkyu 1994 irosaki University, by Grant-in Aid from the Ministry of Education, Science and Culture, Japan. "Inve

Teruo Sano：“类病毒致病性机制分析及其在类病毒疾病控制中的应用”研究报告，Tokutei-Kenkyu 1994 irosaki 大学，由日本文部科学省资助。