家蚕における連鎖球菌感染症の感染病理に関する研究

家蚕链球菌感染的感染病理学研究

• 批准号: 05660064
• 负责人: 松本 継男
• 金额: $ 1.41万
• 依托单位: Kyoto Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660064/
• 关键词: 家蚕; 腸球菌; 血清型別; β溶血株; Enterococci; E.faecalis; E.faecium; E.casselifluvus

D群連鎖状球菌(Enterococcus,腸球菌)は、多くの昆虫の腸管内に生息し腸内細菌叢を構成する主要な細菌の一員であるとともに、昆中疾病の発現に深く関与する通性病原細菌である。家蚕では消化器病(胃腸病)の起因菌とされ、従来から本疾病の発病様相について数多くの検討がなされてきたが、今日までその感染の成立機序については十分に整理されていない。本研究は腸球菌による家蚕消化器病の感染機序を理解するために、病原学的側面より精査しいくつかの新知見を得た。すなわち蚕児の発育経過に伴って腸管内に生息する腸球菌の経時的変化を明らかにするとともに、分離した92株のEnterococciについてその性状を調べ分類学的位置付けを明確にし感染機序究明の足掛かりを示した。まず消化管内の腸球菌は蚕児の摂食桑量に応じて菌量消長を示し、不摂食の眠期や蛹期、成虫期には棲息しないか僅少の残留を認めることが分かった。また採取した分離株は、形態学的および生理生化学的性状とビルレンス因子より8群に大別され、そのなかで運動性を有し非溶血性で旺盛なプロテアーゼ産生を示す菌株が主要な群を形成していた。またα溶血株やβ溶血株、さらに黄色色素産生株も分布していた。このβ溶血株や黄色色素産生株は昆虫由来株としては、最初の記載であった。E.faecalisやE.faeciumの標準菌株を用いて免疫抗血清を調製し分離菌の血清型別を行ったところ、主要菌群はE.faecalis type1抗血清に凝集したが数株はtype6に凝集した。type1は自然界に広く分布し臨床上でも重要な菌型とされているが、type6は希有な菌型で昆虫特異菌型として「生態型別」できる可能性が得られた。API-STREP 20により分離菌を確認したところ、E.faecalisのほかE.faecium,E.casselifluvus,E.duransなどが同定された。これら蚕児腸管内の腸球菌の性状調査から、腸球菌感染症を解明するいくつかの糸口が得られた。

Group D Enterococcus (Streptococcus) and polyphagous insects were infected by bacteria in the tube to form one of the main pathogens, and the diseases in Kunzhong showed deep disease and common pathogens. The digestive organ disease (stomach disease) of the silkworm, Bombyx mori, is caused by bacteria, the disease, the digestive organ disease (stomach disease) of the silkworm, Bombyx mori, Bombyx mori. In this study, the infection mechanism of digestive diseases of silkworm, Bombyx mori, Bombyx mori and etiology were studied. The position of character classification of 92 strains of Enterococci isolated from each other was clearly confirmed by the sequence of infection and the sequence of infection was confirmed. The growth and decline of the number of bacteria in the digestive tract showed the growth and decline of the mulberry quantity, the dormant stage, the pupa stage and the adult stage. The physiological and biochemical characteristics of the isolated strains were isolated, the physiological and biochemical characteristics of the isolates were isolated, the physiological and biochemical characteristics of the strains were isolated, the physiological and biochemical characteristics of the strains were isolated, the physiological and biochemical characteristics of the strains were isolated, the physiological and biochemical characteristics of the strains were isolated, the physiological and biochemical characteristics of the strains were isolated, the physiological and biochemical characteristics of the strains were isolated, the physiological and biochemical characteristics of the strains were isolated, the physiological and biochemical characteristics of the strains were isolated, the physiological and biochemical characteristics of the strains were isolated, the physiological and biochemical characteristics of the strains were isolated, the physiological and biochemical characteristics of the strains were isolated, the physiological and biochemical characteristics of the strains were isolated, the physiological and biochemical characteristics of the strains were isolated, the physiological and biochemical characteristics of the strains were isolated, the physiological and biochemical characteristics of the strains were isolated, the physiological and biochemical characteristics of the strains were isolated, The distribution of α-hemolytic strain, β-hemolytic strain and yellow pigment-producing strain were similar to each other. The β-hemolytic strain, the yellow pigment strain, the insect plant, the insect plant, the hemolytic strain, the yellow pigment strain and the insect strain. The standard strains of E.faecalis "E.faecium" were tested with immune antiserum "isolates", and several strains of main flora "E.faecalis type1 antiserum" agglutination "type6". The distribution of type1 in nature shows that there is a significant increase in the possibility of important bacterial types in the bed, and that type6 is expected to have fungal types, insect specific types, biotypes and phenotypes. The API-STREP 20 isolates confirm that the spores are the same as those of the E.faecalis spores. The characteristics of cocci in the silkworm tube and the infection of cocci were explained.

项目成果

魯興萌 他: "カイコ由来のEnterococcus faecalisの血清型別" 日本蚕糸学雑誌. (印刷中). (1994)

卢兴猛等：“蚕粪肠球菌的血清分型”，日本血清学杂志（1994 年出版）。