权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

NUTRITION METABOLISM OF HIGHER ANIMALS IN HYPOXIA

高等动物缺氧时的营养代谢

基本信息

批准号：
05660146
负责人：
NAKANO Yoshihisa
金额：
$ 1.47万
依托单位：
Osaka Prefecture University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

This study examined the effects of hypoxia on the gastric function in conscious rats in a series of experiments. Hypoxia delayd the excretion rats of urinary urea until after 12h of hypoxia and the delay was more remarkable in rats subjected to 7.6%O_2 hypoxia than in those subjected to 10.5%O_2 hypoxia. In Experiment 2, when rats fed the high protein diet for 4h were exposed to 7.6%O_2, a delay in gastric emptying was found in the hypoxic rats. In Experiment 3, when rats fed the normal protein diet for 4h were exposed to 7.6%O_2 hypoxia, the rats of urea excretion in the hypoxic rats were not significantly different from that in the normoxic rats until after 24h of hypoxia. However, rats fed the normal protein diet for 4h werecaused a delay of gastric emptying by 10.5%O_2 hypoxia. In Experiment 4, when rats fed the high protein diet for 4h were exposed to 7.6%O_2 or 10.5%O_2 hypoxia, the plasma gastrin concentration in the 7.6%O_2 hypoxic rats was 2.3-fold that in the normoxic rats after 6h of hypoxia although the gastrin level did not differ between the 10.5%O_2 hypoxic and the normoxic rats during 6h of hopoxia. Furthermore, when food-deprived rats were exposed to 7.6%O_2 or 10.5%O_2 hypoxia, the gastrin concentration was higher in both hypoxic groups than in the normoxic group after 3h of hypoxia. In Experiment 5, food-deprived rats with pylous ligation were exposed to 7.6%O_2 or 10.5%O_2 hypoxia for 3h. Both hypoxia inhibited the secretion of gastric acid and stimulated the gastrin release. In Experiment 6, food-deprived rats with pylorus ligation were orally administered HC1 and then exposed to 7.6%O_2 hypoxia for 3h. The rise of the gastrin concentration due to hypoxia was completely inhibited by the oral administration of HC1. These results demonstrate that hypoxia inhibits gastric emptying and secretion and that the inhibitory effect of hypoxia on gastric acid secretion stimulates gastrin release through positive feedback regulation.

本研究通过一系列实验研究了缺氧对清醒大鼠胃功能的影响。缺氧使大鼠尿尿素排泄延迟至缺氧12h后，且7.6%O_2缺氧组比10.5%O_2缺氧组延迟更显著。实验2：高蛋白饮食4小时后，低氧大鼠胃排空延迟。在实验3中，将正常蛋白质饲料喂养4h后，暴露于7.6%O_2缺氧环境中，缺氧大鼠的尿素排泄量与正常缺氧大鼠的尿素排泄量在缺氧24h后无显著差异。而在正常蛋白质饮食4小时后，10.5%O_2缺氧导致大鼠胃排空延迟。实验4，高蛋白饲料大鼠在7.6%或10.5%缺氧条件下，缺氧6h后，7.6%缺氧大鼠血浆胃泌素浓度是正常大鼠的2.3倍，而缺氧6h时，10.5%缺氧大鼠血浆胃泌素水平与正常大鼠无显著差异。缺氧3h后，缺氧组和缺氧组胃泌素浓度均高于缺氧组。实验5：将幽门结扎术后无食物大鼠分别置于7.6%或10.5%的缺氧条件下3h。缺氧均抑制胃酸分泌，刺激胃泌素释放。实验六：幽门结扎术后空腹大鼠口服HC1，然后于7.6%O_2缺氧3h。低氧引起的胃泌素浓度升高被口服HC1完全抑制。这些结果表明，缺氧抑制胃排空和分泌，缺氧对胃酸分泌的抑制作用通过正反馈调节刺激胃泌素释放。