Reproductive Biology of the Japanese Common Squid, Todarodes pacificus by Captive Experiments

通过圈养实验研究日本鱿鱼 Todarodes pacificus 的生殖生物学

• 批准号: 05660195
• 负责人: SAKURAI Yasunori
• 金额: $ 1.34万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660195/
• 关键词: Todarodes pacificus; Reproduction; Captive experiment; Spawning behavior; Gelatinuous egg mass; Artificial fertilization; Embryonic development; Paralarvae; 発生適水温; 初期餌料; スルメイカの飼育実験; 氷温麻酔; 産卵特性; 輸卵管腺ゼリー; 成熟促進; 囲卵腔形成; 人工授精技術開発; 卵発生条件

During 1993 to 1996 captive experiments of the Japanese common squid, Todarodes pacificus to clarify the reproductive biology, I get some new informations about the maturation process of captive female, the egg mass formation by spawning and the artificial fertilizatin and development through hatching in this species. The results are as follows.1. Maturation of female : Copulation by male do not accelerate yolk formation of oocytes in oavry. The higher temperarture in captivity may promote the ovulation and the spawning.2. Egg mass formation by spawning : The female can produce gelatinous egg masses, about 60 to 80cm in diameter, within 8 minutes. Egg masses were estimated ontained about 100,000 to 200,000 ova. The ova lay in an appearently uniform mass of gelatinuous substance that originated from the oviducal gland. This was covered by the surface layr of jelly for the nidamental gland.3. Characteristics of egg mass : Egg masses were slightly buoyant and not broken until hatching. Ex … More amination of the egg mass surface layr revealed that the outer nidamental gland gel was effectve in preventing crustaceans, protozoans, and bacteria from entiring the egg mass.4. Effect of temperature on development and survival of paralarvae : We exploited a new artificial fertilization technique on ommastrephid squids. The embryonic development and survival of T.pacificus paralarvae were examined at several temperatures between 3ﾟC to 29ﾟC.Normal embryonic development occurred at temperatures between 14.0 to 26ﾟC,with highest embryonic survival rates occurring between 14.7 and 22.2ﾟC.5. Survival and behavior of paralarvae : Paralarvae survived up to 13 days after hatching, with the highest survival rates occurring at 15ﾟC.Hatching paralarvae swim upward immediately, with many animals found concentrated along the surface layr. We saw no evidence of any feeding by the paralarvae within the egg mass after hatching, however the longer develoment time within the egg mass indicates greater opportunity for pre-hatching paralarvae absorbing organics from the mucus originated from the oviducal gland gel. Less

在1993年至1996年期间，日本普通鱿鱼的圈养实验，即Pacificus Todarodes Pacificus阐明了生殖生物学，我得到了一些有关圈养女性成熟过程的新信息，产卵和人工肥的卵质量形成以及通过这种物种的孵化。结果如下1。女性的成熟：雄性交配不会加速燕麦中的卵母细胞的蛋黄。囚禁的较高温度可以促进排卵和产卵2。通过产卵形成鸡蛋质量：雌性可以在8分钟内产生直径约60至80厘米的凝胶状卵子。估计将卵块分成约100,000至200,000个OVA。 OVA含有源自卵形腺的凝胶状物质的外观均匀质量。这被果冻的表面外层覆盖，以构成NidaMental腺体3。鸡蛋质量的特征：卵质量有点浮力，直到孵化才破裂。前…对卵质量表面的更多胺化表明，外部腺体凝胶有效防止甲壳类动物，原生动物和细菌进入卵质量4。温度对副群的发育和存活的影响：我们利用了一种新的人工施肥技术，对乌剂鱿鱼。在3°C至29°C的几个温度下，在14.0至26°C之间的几个温度下检查了t.pacificus paralarvae的胚胎发育和存活。瘫痪的生存和行为：孵化后长达13天存活，生存率最高的率为15°C。伴有paralarvae立即向上游动，许多动物沿着表面层次发现浓缩。我们没有看到孵化后卵形群中的副群在卵质量内进食的证据，但是鸡蛋质量内的发育时间较长，这表明较大的前群副虫吸收来自粘液的有机物的机会来自卵形腺体凝胶。较少的

项目成果

Sakurai, Y., J.R.Bower, Y.Nakamura, S.Yamamoto and K.Watanabe: "Effects of temperarture on development and survival of Japanese common squid (Todarodes pacificus) paralarvae." J.of American Malacological Bulletin. (accepted). (1996)

Sakurai, Y.、J.R.Bower、Y.Nakamura、S.Yamamoto 和 K.Watanabe：“温度对日本鱿鱼（Todarodes pacificus）副幼虫发育和生存的影响。”

Sakurai, Y., R. E. Young, J. Hirota, K. Mangold, M. Veochione, M. R. Clarke and J. Bower: "Artificial fertilization and development through hatching in the oceanic squids Ommastrephes bartramii and Sthenoteuthis oualaniensis." The Veliger. 38. 185-191 (19

Sakurai, Y.、R. E. Young、J. Hirota、K. Mangold、M. Veochione、M. R. Clarke 和 J. Bower：“通过孵化海洋鱿鱼 Ommastrephes bartramii 和 Sthenoteuthis oualaniensis 进行人工受精和发育。”

Ikeda, Y., Y. Sakurai and K. Shimazaki: "Maturational process of the Japanese common squid, Todarodes pacificus in captivity." Recent Advances in Cephalopod Fisheries Biology, ed. by T. Okutani, R. K. O' Dor and T. Kobodera, Publ. by Tokai University Pres

Ikeda, Y.、Y. Sakurai 和 K. Shimazaki：“日本普通鱿鱼 Todarodes pacificus 圈养的成熟过程。”

桜井泰憲・池田譲: "スルメイカの生態研究における飼育実験法,イカ類の資源・生態研究にインパクトを与えるであろう最近の技術." 平成4年度イカ類資源・漁海況検討会議研究報告,平成4年度,. 51-69 (1994)

Yasunori Sakurai 和 Yuzuru Ikeda：“日本普通鱿鱼生态研究的实验饲养方法，将对鱿鱼资源和生态研究产生影响的最新技术。” 1992 年平成鱿鱼资源和渔业和海洋条件研究小组的研究报告第四年，51-69（1994）

Bower, J.R.and Y.Sakurai: "Laboratory observations of egg masses of the Japanese common squid, Todarodes pacificus (Cepahlopoda : Ommastrephidae)." J.of American Malacological Bulletin. (accepted). (1996)

Bower, J.R. 和 Y.Sakurai：“日本普通鱿鱼 Todarodes pacificus（头足纲：Ommastrephidae）卵块的实验室观察。”