Reproductive Biology of the Japanese Common Squid, Todarodes pacificus by Captive Experiments

通过圈养实验研究日本鱿鱼 Todarodes pacificus 的生殖生物学

• 批准号: 05660195
• 负责人: SAKURAI Yasunori
• 金额: $ 1.34万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660195/
• 关键词: Todarodes pacificus; Reproduction; Captive experiment; Spawning behavior; Gelatinuous egg mass; Artificial fertilization; Embryonic development; Paralarvae; 発生適水温; 初期餌料; スルメイカの飼育実験; 氷温麻酔; 産卵特性; 輸卵管腺ゼリー; 成熟促進; 囲卵腔形成; 人工授精技術開発; 卵発生条件

During 1993 to 1996 captive experiments of the Japanese common squid, Todarodes pacificus to clarify the reproductive biology, I get some new informations about the maturation process of captive female, the egg mass formation by spawning and the artificial fertilizatin and development through hatching in this species. The results are as follows.1. Maturation of female : Copulation by male do not accelerate yolk formation of oocytes in oavry. The higher temperarture in captivity may promote the ovulation and the spawning.2. Egg mass formation by spawning : The female can produce gelatinous egg masses, about 60 to 80cm in diameter, within 8 minutes. Egg masses were estimated ontained about 100,000 to 200,000 ova. The ova lay in an appearently uniform mass of gelatinuous substance that originated from the oviducal gland. This was covered by the surface layr of jelly for the nidamental gland.3. Characteristics of egg mass : Egg masses were slightly buoyant and not broken until hatching. Ex … More amination of the egg mass surface layr revealed that the outer nidamental gland gel was effectve in preventing crustaceans, protozoans, and bacteria from entiring the egg mass.4. Effect of temperature on development and survival of paralarvae : We exploited a new artificial fertilization technique on ommastrephid squids. The embryonic development and survival of T.pacificus paralarvae were examined at several temperatures between 3ﾟC to 29ﾟC.Normal embryonic development occurred at temperatures between 14.0 to 26ﾟC,with highest embryonic survival rates occurring between 14.7 and 22.2ﾟC.5. Survival and behavior of paralarvae : Paralarvae survived up to 13 days after hatching, with the highest survival rates occurring at 15ﾟC.Hatching paralarvae swim upward immediately, with many animals found concentrated along the surface layr. We saw no evidence of any feeding by the paralarvae within the egg mass after hatching, however the longer develoment time within the egg mass indicates greater opportunity for pre-hatching paralarvae absorbing organics from the mucus originated from the oviducal gland gel. Less

在1993~1996年对日本普通乌贼的圈养实验中，为了阐明其繁殖生物学，对圈养雌性乌贼的成熟过程、产卵形成卵块以及通过孵化人工受精和发育等方面获得了一些新的信息。研究结果如下：1.研究结果。雌性成熟：雄性交配不会加速卵母细胞卵黄的形成。圈养温度越高，可能会促进排卵和产卵。产卵形成卵团：雌性能在8分钟内产生直径约60-80厘米的胶状卵团。据估计，卵块大约有10万到20万个卵子。卵子位于一团明显均匀的胶状物质中，这些物质来自输卵管腺。这被海绵状腺体的果冻表层所覆盖。卵块特征：卵块略有浮力，孵化后才破碎。Ex…卵团表面层的胺化程度越高，越能有效地防止甲壳类、原生动物和细菌侵入卵团。温度对长尾乌贼发育和存活的影响：我们开发了一种新的长吻乌贼人工授精技术。在3~29ﾟC的不同温度下，对太平洋对虾胚胎发育和存活进行了研究。正常胚胎发育在14.0~26ﾟC之间，胚胎存活率最高出现在14.7~22.2ﾟC.5之间。副翼幼体的存活和行为：副翼幼体在孵化后存活长达13天，在15ﾟC时存活率最高。孵化的副翼幼体立即向上游，许多动物集中在表层。我们没有看到孵化后卵团内的副翼幼虫有任何摄食的证据，但卵团内发育的时间越长，表明孵化前的副翼幼虫从来自输卵管腺凝胶的粘液中吸收有机物质的机会更大。较少

项目成果

Sakurai, Y., J.R.Bower, Y.Nakamura, S.Yamamoto and K.Watanabe: "Effects of temperarture on development and survival of Japanese common squid (Todarodes pacificus) paralarvae." J.of American Malacological Bulletin. (accepted). (1996)

Sakurai, Y.、J.R.Bower、Y.Nakamura、S.Yamamoto 和 K.Watanabe：“温度对日本鱿鱼（Todarodes pacificus）副幼虫发育和生存的影响。”

Ikeda, Y., Y. Sakurai and K. Shimazaki: "Maturational process of the Japanese common squid, Todarodes pacificus in captivity." Recent Advances in Cephalopod Fisheries Biology, ed. by T. Okutani, R. K. O' Dor and T. Kobodera, Publ. by Tokai University Pres

Ikeda, Y.、Y. Sakurai 和 K. Shimazaki：“日本普通鱿鱼 Todarodes pacificus 圈养的成熟过程。”

桜井泰憲・池田譲: "スルメイカの生態研究における飼育実験法,イカ類の資源・生態研究にインパクトを与えるであろう最近の技術." 平成4年度イカ類資源・漁海況検討会議研究報告,平成4年度,. 51-69 (1994)

Yasunori Sakurai 和 Yuzuru Ikeda：“日本普通鱿鱼生态研究的实验饲养方法，将对鱿鱼资源和生态研究产生影响的最新技术。” 1992 年平成鱿鱼资源和渔业和海洋条件研究小组的研究报告第四年，51-69（1994）

Sakurai, Y., R. E. Young, J. Hirota, K. Mangold, M. Veochione, M. R. Clarke and J. Bower: "Artificial fertilization and development through hatching in the oceanic squids Ommastrephes bartramii and Sthenoteuthis oualaniensis." The Veliger. 38. 185-191 (19

Sakurai, Y.、R. E. Young、J. Hirota、K. Mangold、M. Veochione、M. R. Clarke 和 J. Bower：“通过孵化海洋鱿鱼 Ommastrephes bartramii 和 Sthenoteuthis oualaniensis 进行人工受精和发育。”

Bower, J.R.and Y.Sakurai: "Laboratory observations of egg masses of the Japanese common squid, Todarodes pacificus (Cepahlopoda : Ommastrephidae)." J.of American Malacological Bulletin. (accepted). (1996)

Bower, J.R. 和 Y.Sakurai：“日本普通鱿鱼 Todarodes pacificus（头足纲：Ommastrephidae）卵块的实验室观察。”