Action of novel non-receptor type protein-tyrosine kinase, p72^<syk>in immune cells.
新型非受体型蛋白酪氨酸激酶 p72^<syk> 在免疫细胞中的作用。
基本信息
- 批准号:05670117
- 负责人:
- 金额:$ 1.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1993
- 资助国家:日本
- 起止时间:1993 至 1994
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We have reported a porcine gene sky encoding a non-receptor type 72-kDa PTK (p72^<syk>) which has a distinct structural character, that is the presence of the second src homology region 2 (SH2) instead of SH3, from other members of this group of PTK.Our efforts have in large part focused on the relationship between Syk in the context of surface receptor-initiated signal transduction in B cell and platelet because of their abundant localization.Stimulation of B lymphocytes through their antigen receptor (BCR) result in rapid increases and induces both an increase of phosphatidylinositol and mobilization of cytoplasmic free calcium. The BCR associates with two classes of tyrosine kinase : Src family kinase and Sky kinase. To dissect the functional roles of these two types of kinase in BCR signaling, Syk-deficient cell and Lyn-deficient cell lines were established. Syk-deficient cells abolished the tyrosine phosphorylation phospholipase C (PLC) -gamma, resulting in no inositol, 1,4,5-tris … More phosphate (IP3) generation, as well as calcium moblization upon receptor stimulation. Crosslinking of BCR on Lyn-deficient cells evoked a delayd and slow calcium moblization, despite the normal kinetics of IP3 turnover. These results demonstrated that Syk mediates IP3 generation, whereas Lyn regulates calcium mobization through a process independent of IP3 generation.Activation of Syk in platelets is not restricted to thrombin stimulation. Platelet activating factor, thromboxane A2, wheat germ agglutinin, and collagen also activate Syk. These findings imply that Syk may be one of the key molecules through which various receptor signaling acts. Tyrosine phosphorylation of p125^<FAK> is followed by Syk activation upon integrin receptor stimulation, suggesting that phosphorylation of p125^<FAK> may be mediated by Syk. Reinforcing this idea, the physical association of Syk family PTKs with Src family PTKs or FAK family protein is observed. Furthermore, stimulation of platelets by thrombin induces the rapid translocation of Syk from the cytosolic fraction to the membrane and cytoskeletal fraction. This recruitment of Syk to membrane and cytoskeletal fraction may contribute to the thrombininduced activation of Syk. Less
我们已经报道了一个编码非受体型72-kDa PTK(p72 α)的猪基因sky<syk>,该基因具有独特的结构特征,即存在第二个src同源区2(SH 2)而不是SH 3,由于Syk在B细胞和血小板中的丰富定位,我们的工作主要集中在Syk在B淋巴细胞通过其抗原受体(BCR)激活的信号转导中的作用。导致快速增加并诱导磷脂酰肌醇的增加和细胞质游离钙的动员。BCR与两类酪氨酸激酶相关:Src家族激酶和Sky激酶。为了剖析这两种类型的激酶在BCR信号传导中的功能作用,建立了Syk缺陷细胞和Lyn缺陷细胞系。Syk缺陷细胞消除了酪氨酸磷酸化磷脂酶C(PLC)-γ,导致没有肌醇,1,4,5-三羟甲基氨基甲烷, ...更多信息 磷酸盐(IP 3)的产生以及受体刺激后的钙移动。尽管IP 3的周转动力学正常,但BCR在Lyn-deficient细胞上的交联引起了延迟和缓慢的钙流动。这些结果表明Syk介导IP 3的产生,而林恩通过独立于IP 3产生的过程调节钙的流动。血小板活化因子、血栓素A2、麦胚凝集素和胶原蛋白也可激活Syk。这些发现暗示Syk可能是多种受体信号传导作用的关键分子之一。在<FAK>整合素受体刺激后,p125^的酪氨酸磷酸化之后是Syk活化,表明p125^的磷酸化<FAK>可能由Syk介导。加强这一想法,观察到Syk家族PTKs与Src家族PTKs或FAK家族蛋白的物理关联。此外,凝血酶对血小板的刺激诱导Syk从胞质组分快速易位到膜和细胞骨架组分。这种Syk向细胞膜和细胞骨架的募集可能有助于凝血酶诱导的Syk活化。少
项目成果
期刊论文数量(178)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Maeda,H.: "Protein-tyrosine kinase p72syk is activated by thromboxane A2 mimetic U44069 in platelets." Biochem.Biophys.Res.Commun.197. 62-67 (1993)
Maeda,H.:“蛋白质酪氨酸激酶 p72syk 被血小板中的血栓素 A2 模拟物 U44069 激活。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Maeda, H., Taniguchi, T., Inazu, T., Yang, C.Nakagawara and Yamamura, H.: "Protein-tyrosine kinase p72syk is activated by thromboxane A2 mimetic U44069 in platelets." Biochem.Biophys.Res.Commun.197(1). 62-67 (1993)
Maeda, H.、Taniguchi, T.、Inazu, T.、Yang, C.Nakakawara 和 Yamamura, H.:“蛋白质酪氨酸激酶 p72syk 被血小板中的血栓素 A2 模拟物 U44069 激活。”
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- 影响因子:0
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- 通讯作者:
Fujii, C., Yanagi, S., Sada, K., Nagai, K., Taniguchi, T.and Yamamura, H.: "Protein-tyrosine kinase p72syk.is activated by collagen in platelets" Eur.J.Biochem.226. 243-248 (1994)
Fujii, C.、Yanagi, S.、Sada, K.、Nagai, K.、Taniguchi, T. 和 Yamamura, H.:“蛋白质酪氨酸激酶 p72syk. 被血小板中的胶原蛋白激活”Eur.J.Biochem。
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- 影响因子:0
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- 通讯作者:
Minami, Y., Nakagawa, Y., Kawahara, A., Miyazaki, T., Sada, K., Yamamura, H.and Taniguchi, T.: "Protein Tyrosine Kinase Syk Is Associated with and Activated by The IL-2 Receptor ; Possible Link with The c-myc Induction Pathway." Immunity. (in press).
Minami, Y.、Nakakawa, Y.、Kawahara, A.、Miyazaki, T.、Sada, K.、Yamamura, H. 和 Taniguchi, T.:“蛋白质酪氨酸激酶 Syk 与 IL-2 相关并由其激活
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- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Fujii,C.: "Protein-tyrosine kinase p72syk. is activated by collagen in platelets." Eur.J.Biochem.226. 243-248 (1994)
Fujii,C.:“蛋白质酪氨酸激酶 p72syk. 被血小板中的胶原蛋白激活。”
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YANAGI Shigeru其他文献
YANAGI Shigeru的其他文献
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{{ truncateString('YANAGI Shigeru', 18)}}的其他基金
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16K15224 - 财政年份:2016
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$ 1.41万 - 项目类别:
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Role of MITOL in mitochondrial dynamics
MITOL 在线粒体动力学中的作用
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26293072 - 财政年份:2014
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Development of CRAG gene therapy for neurodegenerative diseases
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25640040 - 财政年份:2013
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Grant-in-Aid for Challenging Exploratory Research
Role of MITOL in mitochondrial function and oxidative stress signaling
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23650195 - 财政年份:2011
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Neuronal development via redox signaling and mitochondrial dynamics
通过氧化还原信号和线粒体动力学的神经元发育
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20300133 - 财政年份:2008
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$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Elucidation of molecular signaling mechanism of axonal guidance
阐明轴突引导的分子信号机制
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17300117 - 财政年份:2005
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$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Elucidation of molecular signaling mechanism of axonal guidance
阐明轴突引导的分子信号机制
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15590247 - 财政年份:2003
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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