The network of immune system and nerve system in host defense to bacterial infections

宿主防御细菌感染的免疫系统和神经系统网络

• 批准号: 05670245
• 负责人: NAKANE Akio
• 金额: $ 1.41万
• 依托单位: Hirosaki University (1994-1995)Hokkaido University (1993)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670245/
• 关键词: Neuropeptide; Substsnce P; Vasoactive intestinal peptide; Listeria monocytogenes; Tsukamullela paurometabolum; Mice; Host defense; Bacterial infection; リステリア; RT-PCR; 感染; 細菌; ロドコッカス

The network is formed between immune system, nerve system, and endocrine system. We were interested in the interaction between immune system and nerve system in bacterial infections. We chose the neuropeptides such as substance P (SP) and vasoactive intestinal peptide (VIP) as the parameter of nerve system. We investigated the induction of SP and VIP en the livers of mice which were infected intravenously with Listeria monocytogenes or Tsukamullela paurometabolum.In the studies using an immunohistochemical technique, neither SP nor VIP was detected in the livers of uninfected mice. VIP,but not SP was detected around the blood vessels and in the surrounding infiltrating cells of livers obtained from Listeria-infected mice. In contrast, SP,but not VIP,was detected in the granuloma-like lesions of livers obtained from Tsukamullela-infected mice. These result suggest that the selective induction of SP and VIP might occur, depending on bacteria infected, and that the neuropeptides might be secreted by the inflammatory cells. In the studies using reverse transcription-PCR,propre VIP mRNA was induced in parallel with the growth of Listeria cells in the livers.These results demonstrate that the neuropeptides might be involved in the inflammatory process and/or the host defense in bacterial infections.

免疫系统、神经系统和内分泌系统之间形成网络。我们感兴趣的是在细菌感染中免疫系统和神经系统之间的相互作用。选择P物质（SP）、血管活性肠肽（VIP）等神经肽作为神经系统参数。我们研究了单核细胞增生李斯特菌和少代谢冢口氏菌经静脉感染小鼠后，其肝脏中SP和VIP的诱导作用，在免疫组织化学研究中，未感染小鼠的肝脏中未检测到SP和VIP。从李斯特菌感染的小鼠获得的肝脏血管周围和周围浸润细胞中检测到VIP，但未检测到SP。与此相反，SP，而不是VIP，检测到从Tsukamullella感染小鼠的肝脏肉芽肿样病变。这些结果表明，SP和VIP的选择性诱导可能取决于感染的细菌，并且神经肽可能由炎性细胞分泌。在逆转录-PCR研究中，VIP前体mRNA的表达与李斯特菌在肝脏中的生长同步，提示VIP前体可能参与了细菌感染的炎症过程和/或宿主防御。

项目成果

Akio Nakane: "Vasoactive intestinal peptide in the livers of mice during Listeria monocytogenes infection" (投稿準備中).

Akio Nakane：“单核细胞增生李斯特菌感染期间小鼠肝脏中的血管活性肠肽”（准备提交）。

Akio Nakane: "Vasoactive intestinal peptide in the livers of mice during Listeria monocytogenes infection" (Manuscript in preparation).

Akio Nakane：“单核细胞增生李斯特菌感染期间小鼠肝脏中的血管活性肠肽”（手稿正在准备中）。

Masashi Kohanawa: "CD3+/TCR-αβ-cells are important in protecting spinal cord tissues against Theiler′s virus strain GDVII infection" Microbiol.Immunol.39. 123-128 (1995)

Masashi Kohanawa：“CD3+/TCR-αβ-细胞对于保护脊髓组织免受泰勒病毒株 GDVII 感染非常重要”Microbiol.Immunol.39 (1995)。

Akio Nakane: "A monoclonal antibody against T-cell receptor αβ induces endogenous cytokines and prevents mice from a lethal infection with Listeria monocytogenes" FEMS Immunology and Medical Microbiology. 11. 321-329 (1995)

Akio Nakane：“针对 T 细胞受体 αβ 的单克隆抗体可诱导内源性细胞因子并防止小鼠受到单核细胞增多性李斯特菌的致命感染”FEMS 免疫学和医学微生物学 11. 321-329 (1995)。

Masashi Kohanawa: "Theiler's virus is eliminated by a gamma-interferon-independent mechanism in the brain" Journal of Neuroimmunology. 52. 79-86 (1994)

Masashi Kohanawa：“泰勒氏病毒是通过大脑中不依赖γ-干扰素的机制消除的”《神经免疫学杂志》。