Is the superantigenic activity required for the emetic activity of staphylococcal enterotoxin A

葡萄球菌肠毒素A的催吐活性是否需要超抗原活性

• 批准号: 18390132
• 负责人: NAKANE Akio
• 金额: $ 10.98万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390132/
• 关键词: Enterotoxin; Staphylococcus aureus; Superantigen; Emesis; Suncus; Cytokine; 嘔吐

Staphylococcal enterotoxin A (SEA) is an extracellular protein produced by Staphylococcus aureus. SEA shows emetic activity and superantigenic activity. The mechanism of emetic activity of SEA has been little known. In the present study, we demonstrated the following results:Mutation was carried out at sites of T-cell receptor- and MHC class II molecule-binding regions on SEA by site-directed mutagenesis. We prepared 13 mutant SEA (mSEA) proteins. We examined superantigenic activities of mSEAs in human peripheral blood leukocytes (HPBL) and spleen cells of house musk shrews (Suncus murinus). Moreover, we compared emesis-inducing activities among these mSEAs by intraperitoneal injection. All mSEAs reacted with anti-SEA antibody. Superantigenic activities of mSEAs including N25G, F47G, C96G, C106A, V174G and D227A were deficient because cell proliferation and induction of IL-2, IFN-γ and TNF-α in HPBL were reduced in these mutant proteins. Activities of cell proliferation to spleen cell of house musk shew were reduced in mSEA including F47G, F47S, C96G, C106A, V174G and D227A. Emesis-inducing activities to house musk shew were reduced in F47G, F47S, H61D, H187A and D227A. These results suggest that sites of F47 and D227 on a SEA molecule are essential for both superantigenic and emesis-inducing activities and of H61, H187 and H225 for emesis-inducing activity and that active sites for superantigenic and emesis-inducing activities may be different.

金黄色葡萄球菌肠毒素A(SEA)是金黄色葡萄球菌产生的一种胞外蛋白。SEA具有催吐活性和超抗原性。SEA的催吐活性机制一直知之甚少。在本研究中，我们证明了以下结果：通过定点突变，在SEA上T细胞受体和MHC II类分子结合区的位置发生了突变。我们制备了13个突变的SEA(MSEA)蛋白。我们检测了mSEAs在人外周血白细胞(HPBL)和家麝鼠(Suncus Murinus)脾细胞中的超抗原活性。此外，我们通过腹腔注射比较了这些mSEA之间的呕吐诱导活性。所有mSEA均与抗SEA抗体反应。N25G、F47G、C96G、C106A、V174G和D227A等mSEA的超抗原性缺失是因为这些突变蛋白降低了细胞增殖和诱导HPBL产生IL-2、干扰素-γ和肿瘤坏死因子-α。MSEA中F47G、F47S、C96G、C106A、V174G和D227A的细胞对麝香脾细胞的增殖活性降低。F47G、F47S、H61D、H187A和D227A对麝香的诱吐活性降低。这些结果表明，SEA分子上的F47和D227位点对超抗原性和诱发呕吐活性是必需的，H61、H187和H225对诱发呕吐活性是必需的，并且超抗原活性和诱发呕吐活性的活性部位可能不同。

项目成果

黄色ブドウ球菌接着因子による感染予防効果

金黄色葡萄球菌粘附因子的预防感染作用

• 发表时间: 2008
• 作者: 成田浩司;胡東良;重茂克彦;品川邦汎;中根明夫
• 通讯作者: 中根明夫

ブドウ球菌エンテロトキシンの嘔吐誘導活性とスーパー抗原活性の関連性

葡萄球菌肠毒素的呕吐活性与超抗原活性的关系

• 发表时间: 2008
• 作者: 胡東良;重茂克彦;品川邦汎;中根明夫
• 通讯作者: 中根明夫

The protective effect of intranasal vaccination with nontoxic mutant TSST-1 on systemic and local Staphylococcus aureus infections

鼻内接种无毒突变体TSST-1对全身和局部金黄色葡萄球菌感染的保护作用

• 发表时间: 2007
• 作者: Narita K;ed. al.
• 通讯作者: ed. al.

無毒変異TSST-1経鼻粘膜ワクチンによる黄色ブドウ球菌感染防御効果

无毒突变型TSST-1鼻粘膜疫苗预防金黄色葡萄球菌感染的效果

• 发表时间: 2007
• 作者: 成田浩司;胡東良;辻孝雄;中根明夫
• 通讯作者: 中根明夫

Protective effect of intranasal vaccination with nontoxic mutant toxic shock syndrome toxin 1 against systemic and local staphylococcus aureus infection.

鼻内接种无毒突变型中毒性休克综合征毒素 1 对全身和局部金黄色葡萄球菌感染的保护作用。

• 发表时间: 2008
• 期刊: Hirosaki Medical. Journal 59
• 作者: Narita;K and Nakane;A ;et. al.
• 通讯作者: et. al.