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Morphometric Studies on Autolysis of the Cerebellar Cortex in Brain Death by Three-dimensional Color Image Analysis System

三维彩色图像分析系统对脑死亡小脑皮质自溶的形态学研究

基本信息

批准号：
05670399
负责人：
FURUKAWA Masataka
金额：
$ 1.41万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670399/
关键词：
Brain death Cerebellum Granular layr Autolysis Color image analysis 三次元画像解析

项目摘要

Among the issues that concern brain death and organ transplants in Japan. one that is apt to be the subject of controversy is whether or not a brain death pronouncement has been made prematurely. The subject of our study has been the guideline for making this pronouncement based on the correlation between elapsed time and granular layr autolysis (GLA) in the cerebellum. For this research. we employed a color image analysis system manufactured by Olympus.1. On Two-Dimensional Image AnalysisWe investigated normal brain control specimens and brain dead cerebellum samples by comparing the granule cell (G) count in the granular layr of both. As a result, the G count in the case of the brain dead samples declined markedly in proportion to the time elapsed subsequent to pronounced brain death. and this is believed to be an effective indicator that shows the duration of the brain dead state.2. On Three-Dimensional Image AnalysisWe made investigation into the outer and inner strata of the cerebellum of brain dead cases, and, on the basis of their GLA characteristics, we grouped our investigations thus : (1) types where autolysis of erythrocytes and granular cells is complete, (2) - (4) types where erythrocytes and granular cells survive to some degree. As a result, groups (2) - (4) are believed to be effective indicators in the estimation of the duration of brain dead state for the outer cerebellum strata. We then attempted a three dimensional image reconstruction of cross-section specimens of all data for both normal and brain dead cases. As a result.in the brain dead cases.we discovered GLA believed to arise from interruption of blood flow in arteriolar peripheries where thrombi are present.

在日本，脑死亡和器官移植的问题。其中一个容易引起争议的问题是，是否过早地宣布了脑死亡。我们的研究主题是根据经过时间与小脑颗粒层自溶（GLA）之间的相关性做出这一声明的指南。为了这项研究。我们使用了由Cumulus制造的彩色图像分析系统。本文对正常脑组织和脑死亡小脑组织颗粒层的颗粒细胞（G）计数进行了比较。结果，在脑死亡样品的情况下，G计数与明显脑死亡后经过的时间成比例地显著下降。这被认为是显示脑死亡状态持续时间的有效指标。关于三维图像分析我们对脑死亡病例的小脑外层和内层进行了调查，并根据其GLA特征将我们的调查分为：（1）红细胞和颗粒细胞自溶完成的类型，（2）-（4）红细胞和颗粒细胞在一定程度上存活的类型。因此，认为组（2）-（4）是估计小脑外层脑死亡状态持续时间的有效指标。然后，我们尝试了一个三维图像重建的横截面标本的所有数据为正常和脑死亡的情况下。在脑死亡病例result.in，我们发现GLA被认为是由于血栓存在的小动脉周围血流中断引起的。