Control of movements by the cerebellum

这是一份行政�VE附录的请求，以促进与健康相关的研究的多样性 目前的ac�ve奖励1R37NS128416以支持fi的一年级研究生Alden Naeem 约翰霍普金斯医学院的生物医学工程项目。她的多样性资格 补充品是基于她整个童年所承受的经济劣势， 青春期和本科。我们为奥尔登·纳伊姆提出的研究项目是 在非人类灵长类动物身上制作小脑疾病模型，然后使用该模型对Quan�FY Ac�vi�Es在清醒行为动物模型患病的小脑细胞。如果成功，这将是 导致了fi首次在非人类灵长类动物中建立了发作性小脑疾病模型。 我们将采用的方法是使用化学基因�c技术来瞬时和可逆地， 操纵猴小脑浦肯野细胞的�活性。一个次要目标是比较 Ac�vi�Es前小脑细胞和�er浦肯野细胞沉默。重要的是，我们将能够 在同一个神经元中进行这种比较是因为化学基因�c沉默的作用很短。 活着。通过记录P细胞沉默时小脑神经元的Ac�vi�Es，我们将 能够测试P细胞及其下游的峰电流模数�ON之间的因果联系 核神经元，以及行为变化，如终点运动的准确性。

This is a request for a Administra�ve Supplement to Promote Diversity in Health-Related Research for the currently ac�ve award 1R37NS128416 to support Alden Naeem, a first-year graduate student in the Biomedical Engineering Program at the Johns Hopkins School of Medicine. Her eligibility for the diversity supplement is based on economic disadvantages that she endured throughout her childhood, adolescence, and undergraduate years. The research project that we propose for Alden Naeem is to produce a model of cerebellar disease in a non-human primate, and then use the model to quan�fy ac�vi�es of cells in the model-diseased cerebellum in awake behaving animals. If successful, this will result in a first-ever model of episodic cerebellar disease in a non-human primate. The approach that we will employ is to use chemogene�c technology to transiently, and reversibly, manipulate the ac�vity of Purkinje cells in the cerebellum of marmosets. A sub aim is to compare the ac�vi�es of cells in the cerebellum before and a�er Purkinje cell silencing. Importantly, we will be able to do this comparison in the same neuron because the effects of the chemogene�c silencing are short lived. By recording the ac�vi�es of neurons in the cerebellum when the P-cells are silenced, we will be able to test for a causal link between modula�on of spike rates in the P-cells and their downstream nucleus neurons, and behavioral changes such as endpoint accuracy of movements.