Control of movements by the cerebellum

小脑对运动的控制

• 批准号: 10842088
• 负责人: REZA SHADMEHR
• 金额: $ 6.6万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-15 至 2027-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10842088
• 关键词: Adolescence; Animals; Award; Behavioral; Biomedical Engineering; Callithrix; Cell Nucleus; Cells; Cerebellar Diseases; Cerebellum; Childhood; Disease model; Eligibility Determination; Health; Link; Modeling; Movement; Neurons; Purkinje Cells; Research; Research Project Grants; Technology; Testing; awake; economic disparity; embryonic stem cell; graduate student; medical schools; nonhuman primate; programs; undergraduate student

This is a request for a Administra�ve Supplement to Promote Diversity in Health-Related Research for the currently ac�ve award 1R37NS128416 to support Alden Naeem, a first-year graduate student in the Biomedical Engineering Program at the Johns Hopkins School of Medicine. Her eligibility for the diversity supplement is based on economic disadvantages that she endured throughout her childhood, adolescence, and undergraduate years. The research project that we propose for Alden Naeem is to produce a model of cerebellar disease in a non-human primate, and then use the model to quan�fy ac�vi�es of cells in the model-diseased cerebellum in awake behaving animals. If successful, this will result in a first-ever model of episodic cerebellar disease in a non-human primate. The approach that we will employ is to use chemogene�c technology to transiently, and reversibly, manipulate the ac�vity of Purkinje cells in the cerebellum of marmosets. A sub aim is to compare the ac�vi�es of cells in the cerebellum before and a�er Purkinje cell silencing. Importantly, we will be able to do this comparison in the same neuron because the effects of the chemogene�c silencing are short lived. By recording the ac�vi�es of neurons in the cerebellum when the P-cells are silenced, we will be able to test for a causal link between modula�on of spike rates in the P-cells and their downstream nucleus neurons, and behavioral changes such as endpoint accuracy of movements.

这是一份行政补充申请，旨在促进健康相关研究的多样性 目前正在进行的奖项 1R37NS128416 用于支持奥尔登·纳伊姆 (Alden Naeem)，他是该校一年级研究生 约翰霍普金斯大学医学院的生物医学工程项目。她的多元化资格 补助金是基于她整个童年时期所承受的经济困难， 青春期、本科阶段。我们为 Alden Naeem 提出的研究项目是 在非人类灵长类动物中建立小脑疾病模型，然后使用该模型来量化 清醒行为动物模型患病小脑细胞的活动。如果成功的话，这将 首次在非人类灵长类动物中建立了阵发性小脑疾病模型。 我们将采用的方法是使用化学基因技术来瞬时且可逆地 操纵狨猴小脑中浦肯野细胞的活动。一个次要目标是比较 浦肯野细胞沉默前后小脑细胞的活动。重要的是，我们将能够 在同一神经元中进行此比较，因为化学沉默的影响很短 住过。通过记录 P 细胞沉默时小脑神经元的活动，我们将 能够测试 P 细胞及其下游尖峰速率调节之间的因果关系 核神经元和行为变化，例如运动的终点准确性。