Control of movements by the cerebellum

小脑对运动的控制

• 批准号: 10842088
• 负责人: REZA SHADMEHR
• 金额: $ 6.6万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-15 至 2027-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10842088
• 关键词: Adolescence; Animals; Award; Behavioral; Biomedical Engineering; Callithrix; Cell Nucleus; Cells; Cerebellar Diseases; Cerebellum; Childhood; Disease model; Eligibility Determination; Health; Link; Modeling; Movement; Neurons; Purkinje Cells; Research; Research Project Grants; Technology; Testing; awake; economic disparity; embryonic stem cell; graduate student; medical schools; nonhuman primate; programs; undergraduate student

This is a request for a Administra�ve Supplement to Promote Diversity in Health-Related Research for the currently ac�ve award 1R37NS128416 to support Alden Naeem, a first-year graduate student in the Biomedical Engineering Program at the Johns Hopkins School of Medicine. Her eligibility for the diversity supplement is based on economic disadvantages that she endured throughout her childhood, adolescence, and undergraduate years. The research project that we propose for Alden Naeem is to produce a model of cerebellar disease in a non-human primate, and then use the model to quan�fy ac�vi�es of cells in the model-diseased cerebellum in awake behaving animals. If successful, this will result in a first-ever model of episodic cerebellar disease in a non-human primate. The approach that we will employ is to use chemogene�c technology to transiently, and reversibly, manipulate the ac�vity of Purkinje cells in the cerebellum of marmosets. A sub aim is to compare the ac�vi�es of cells in the cerebellum before and a�er Purkinje cell silencing. Importantly, we will be able to do this comparison in the same neuron because the effects of the chemogene�c silencing are short lived. By recording the ac�vi�es of neurons in the cerebellum when the P-cells are silenced, we will be able to test for a causal link between modula�on of spike rates in the P-cells and their downstream nucleus neurons, and behavioral changes such as endpoint accuracy of movements.

这是一份关于促进健康相关研究多样性的行政补充申请， 目前的活动奖1 R37 NS 128416，以支持奥尔登Naeem，一个一年级的研究生在 约翰霍普金斯医学院生物医学工程项目。她的多样性资格 补贴是基于她整个童年时期所遭受的经济困难， 青春期和本科阶段。我们为奥尔登纳伊姆提出的研究项目是 在非人类灵长类动物中建立小脑疾病模型，然后使用该模型进行定量分析。 在清醒行为动物的模型-患病小脑中的细胞凋亡。如果成功，这将 这是有史以来第一次在非人类灵长类动物中建立发作性小脑疾病的模型。 我们将采用的方法是利用化学基因技术， 操纵狨猴小脑中浦肯野细胞的活性。一个子目标是比较 小脑中的细胞消失之前和一个更好的浦肯野细胞沉默。重要的是，我们将能够 我在同一个神经元中做这个比较，因为化学基因沉默的效果很短。 居住通过记录当P细胞沉默时小脑神经元的活动，我们将 能够测试P细胞及其下游细胞中尖峰速率的模块之间的因果关系 核神经元和行为变化，如运动的终点准确性。