HISTOCHEMICAL ANALYSIS OF BLOOD GROUP-RELATED CARBOHYDRATE CHAINS BY USING LECTINS,MONOCLONAL ANTIBODIES COMBINED WITH GLYCOSIDASE DIGESTION

凝集素、单克隆抗体结合糖苷酶消化对血型相关碳水化合物链进行组织化学分析

• 批准号: 05670396
• 负责人: ITO Nobuaki
• 金额: $ 1.02万
• 依托单位: NARA MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670396/
• 关键词: lectin; monoclonal antibody; blood group antigen; carbohydrate antigen; glycosidase; histochemistry; thyroid gland; cancer; ポリラクトサミン

By using endo-beta-galactosidase-GSA-II staining procedure, histochemical demonstration of polylactosamine structure carrying blood group related antigens was carried out in normal human tissues such as pancreas, salivary glands, bronchial glands and stomach. The results revelaed the relationship between the pattern of expression of blood group antigens and endo-beta-galactosidase susceptible poly-N-acetyllactosamine in different normal tissues. By applying this procedure and staining with lectins specific to poly-N-acetyllactosamine such as DSA,PWM and LEA,backbone structures of blood group-related antigens expressed in thyroid neoplasms were examined. ABH related antigens expressed in these neoplasms were susceptible to endo-beta-galactosidase digestion. Endo-beta-galactosidase digestion disclosed the reactivity with GSA-II in thyroid papillary carcinomas but not other types of thyroid neoplasms. In addition, lectins specific to poly-N-acetyllactosamine selectively reacted with papillary carcinomas but not with other types of neoplasms. Thus, lectin or monoclonal antibody staining combined with endo-beta-galactosidase digestion is a powerful and useful means for detecting and analyzing backbone structures carrying blood-group-related antigens in normal and malignant human tissues.

采用内切- β -半乳糖苷酶- gsa - ii染色法，对胰腺、唾液腺、支气管腺、胃等正常人体组织中携带血型相关抗原的多乳糖胺结构进行了组织化学证明。结果揭示了不同正常组织中血型抗原与内切-半乳糖苷酶敏感型聚n -乙酰乳胺的表达模式之间的关系。应用这一方法，用DSA、PWM和LEA等聚n -乙酰乳胺特异性凝集素染色，检测甲状腺肿瘤中表达的血型相关抗原的主干结构。在这些肿瘤中表达的ABH相关抗原易被内切-半乳糖苷酶消化。内切- β -半乳糖苷酶酶切在甲状腺乳头状癌中发现了与GSA-II的反应性，而在其他类型的甲状腺肿瘤中则没有。此外，聚n -乙酰乳胺特异性凝集素选择性地与乳头状癌反应，但与其他类型的肿瘤没有反应。因此，凝集素或单克隆抗体染色结合内切- β -半乳糖苷酶是检测和分析正常和恶性人体组织中携带血型相关抗原的骨干结构的有效手段。

项目成果

Nobuaki ITO et al.: "Histochemical demonstration of endo-β-galactosidase susceptible poly-N-acetyllactosamine with the blood group specificities……" Acta Histochemica et Cytochemica. 27. 451-458 (1994)

Nobuaki ITO 等人：“具有血型特异性的内切 β-半乳糖苷酶敏感聚 N-乙酰基乳糖胺的组织化学证明......” Acta Histochemica et Cytochemica。 27. 451-458 (1994)

MASAKO YOKOTA et al: "Histochemical differences of the lectin affinities of backbone polylactosamine structures carrying the ABO blood group antigens in papillary carcinoma and other types of thyroid neoplasms" Histochem.J. 27. 139-147 (1995)

MASAKO YOKOTA 等人：“乳头状癌和其他类型甲状腺肿瘤中携带 ABO 血型抗原的主链聚乳糖胺结构的凝集素亲和力的组织化学差异” Histochem.J。

Masako Yokota: "Histochemical differences of the lectin affinities of backbone polylactosamine stouctures carrying the ABO blood group artigens in papillary carcinoma and other types of thyroid neoplasms" Histochemial Journal. 27. 139-147 (1995)

Masako Yokota：“乳头状癌和其他类型甲状腺肿瘤中携带 ABO 血型抗原的主链聚乳糖胺结构的凝集素亲和力的组织化学差异”《组织化学杂志》。

NOBUAKI ITO et al: "Localization of blood-group-related linear poly-N-acetyllactosamine structure in different human tissues by Griffonia simplicifolia agglutinin-II staining following endo-beta-galactosidase digestion" Histochem.J. 26. 327-336 (1994)

NOBUAKI ITO 等人：“内切 β-半乳糖苷酶消化后，通过单叶加纳凝集素-II 染色对不同人体组织中血型相关的线性聚 N-乙酰基乳糖胺结构进行定位” Histochem.J。

Nobuaki Ito: "Localization of blood-group-related linear pdy-N-acetyllactosamine structure in different human tissues by Griffonia simplicifolia agglutinine-II staining following endo-β-galactosidase digestion" Histochemical Journal. 26. 327-336 (1994)

Nobuaki Ito：“内切β-半乳糖苷酶消化后通过加纳单叶草凝集素-II 染色定位不同人体组织中与血型相关的线性 pdy-N-乙酰乳糖胺结构”《组织化学杂志》26. 327-336 (1994)。