Analysis of the peptide inside HLA of colonic epithelial cells in inflammatory bowel disease

炎症性肠病结肠上皮细胞HLA内肽的分析

• 批准号: 05670450
• 负责人: KAWABE Takao
• 金额: $ 1.41万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670450/
• 关键词: HLA; inflammatory bowel; peptide; Colonic epithelial cell

In inflammatory bowel diseases, colonic epithelial cells have both class I and class II of HLA on the surface. Recent studies on HLA showed that antigens are located inside HLA as 9-13 aminoacid of peptides. Therefore, we tried to analyze those peptide inside LHA of colonic epithelial cells in patients with inflammatory bowel disease and to elucidate the nature of the antigen recognized by cytotoxic T cell (analysis of class I HLA) or represented by colonic epithelial cells (analysis of class II HLA). We obtained colonic epthelial cells from surgical specimens of patients with inflammatory bowel disease. HLA was extracted from those homogenate cells with affinity chromatography using anti-HLA antibody. We obtained the extract containing HLA.The peptides inside LHA were separated and purified by HPLC.However, its quantity and purity were not enough to analyze the structure and nature of the peptides. We continue to improve the various conditions of the process.

在炎症性肠病中，结肠上皮细胞表面具有HLA I类和II类。近年来对HLA的研究表明，抗原以9-13个氨基酸的多肽形式存在于HLA内。因此，我们试图分析炎症性肠病患者结肠上皮细胞LHA内的这些肽，并阐明细胞毒性T细胞识别的抗原（I类HLA分析）或结肠上皮细胞代表的抗原（II类HLA分析）的性质。我们从炎症性肠病患者的手术标本中获得结肠上皮细胞。用抗HLA抗体进行亲和层析，从这些匀浆细胞中提取HLA。我们获得了含有HLA的提取物，采用高效液相色谱法对LHA中的肽进行了分离纯化，但其数量和纯度不足以分析肽的结构和性质。我们继续改善这一进程的各种条件。

项目成果

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Imai Y，Kawabe T，Omata M，等：“原发性胃绒毛膜癌一例及日本文献综述”J Gastroenterology。

Ota.S, Kawabe T, et al: "Roles of Ca^<2+> and protein kinase C in regulation of prostaglandin E_2 release by mltured rabb.t gastric epithelial alls" Dig.Dis Sci. 38. 1426-1434 (1993)

Ota.S、Kawabe T等人：“Ca 2+ 和蛋白激酶C在调节培养的rabb.t胃上皮细胞释放前列腺素E_2中的作用”Dig.Dis Sci。

Takahashi M, Kawabe T et al: "Hepato cyte growth factor is the most potent endogeneous stimulant of rabbit gastric epithelial cell proliferation and nugration in primary culture." J.Clin Invest. 95. 1994-2003 (1995)

Takahashi M、Kawabe T 等人：“肝细胞生长因子是原代培养中兔胃上皮细胞增殖和成核最有效的内源性刺激剂。”

Ota S,Kawabe T,Omata M,et al: "Roles of Ca^<2+> and protein kinase C in regulation of prostaglandin E_2 release by cultured rabbit gastric epithelial cells." Dig Dis Sci. 38. 1426-1434 (1993)

Ota S，Kawabe T，Omata M，等：“Ca ^ 2 和蛋白激酶C在调节培养兔胃上皮细胞前列腺素E_2释放中的作用。”

Ota S: "Role of Ca^<2+> and protein lcinase C in regulation of prostaglandin E2 release by cultured rabbit gestric epithelial cells" Dig Dis Sci. 38. 1426-34 (1993)

Ota S：“Ca ^ 2 和蛋白纤维素酶C在调节培养的兔胃上皮细胞的前列腺素E2释放中的作用”Dig Dis Sci。