Analysis of ras signal transduction in pancress cancer

胰腺癌ras信号转导分析

• 批准号: 07670567
• 负责人: KAWABE Takao
• 金额: $ 1.66万
• 依托单位: UNIVERSITY of TOKYO
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670567/
• 关键词: pancreas cancer; ras; point mutation; 遺伝子診断; ras遺伝子; 膵管過形成; PCR

A point mutation in the codon 12 of K-ras is detected in 70-80% of pancreas cancers. We previously showed this mutation in pancreatic juice in all the patients with pancreatic cancer using high sesitivite PCR.However, the mutations were also detected in pancreatic juice in 40% of the patients without cancer. We showed the mutation were derived from the hyperplasia of ductal epithelium. In pancratic cancer, the mutated codons were often GGT or TGT,whereas those in non-cancer patients were GGT or TGT.We were confirming this tendency in the further study using larger number of subjects. We also transfected mutated ras into the cells with wild-type ras, and investgated the growth, NDA synsesis, MAP-kinase activity and interaction with Raf 1-kinase in those cells. We continue to elicidate the relation of the mutation type to the cell phenotype, such as cancer, hyperplasia.

K-ras基因第12密码子的点突变在70-80%的胰腺癌中被检测到。我们之前在所有使用高敏感性PCR的胰腺癌患者的胰液中都发现了这种突变，但在40%的非癌症患者的胰液中也检测到了这种突变。我们发现突变是由导管上皮细胞的增生引起的。在胰腺癌中，突变的密码子通常是GGT或TGT，而在非癌症患者中，突变的密码子通常是GGT或TGT。我们在使用更多受试者的进一步研究中证实了这一趋势。我们还将突变的ras基因导入野生型ras基因的细胞中，研究其生长、NDA合成、MAP-K活性以及与RAF-1-K的相互作用。我们继续推断突变类型与细胞表型的关系，例如癌症、增生症。

项目成果

Kawabe T, Tada T et al: "Endoscopic papillary balloon dilation in cirrhotic patients : reval of common bile duct stones withoat sphincterotomy" Endoscopy. 28. 694-498 (1996)

Kawabe T、Tada T 等人：“肝硬化患者的内窥镜乳头状球囊扩张术：不进行括约肌切开术对胆总管结石的再评估”内窥镜检查。

Kawabe T,Tada T,et al: "Endoscopic pallipaly balloon dilation in cirrhotic patients : removal of common bile duct stones without sphineterotomy." Endoscopy. 28. 694-698 (1996)

Kawabe T、Tada T 等人：“肝硬化患者的内镜下苍白球囊扩张：无需乳头括约肌切开术即可去除胆总管结石。”

Tada M,Kawabe T,et al: "Analysis of K-ras gene mutation in hyperplastic duct cells of the pancreas without pancreatic disease" Gastroenterology. 110. 227-237 (1996)

Tada M，Kawabe T，et al：“无胰腺疾病的胰腺增生性导管细胞中 K-ras 基因突变的分析”胃肠病学。

多田 稔、川邊隆夫: "Analysis of K-ras Gene Mutation in Hypei Without Pancreatic Disease" GASTROENTEROLOGY. 110. 227-231 (1996)

Minoru Tada、Takao Kawabe：“无胰腺疾病的 Hypei 中 K-ras 基因突变的分析”胃肠病学。 110. 227-231 (1996)

Tada M, Kawabe T et al: "Analysis of K-ras gene mutation in hyperplastic duct cells of the pancreas with out pan creatic disease" Gastroenterology. 110. 227-231 (1996)

Tada M、Kawabe T 等人：“无胰腺疾病的胰腺增生性导管细胞中 K-ras 基因突变的分析”胃肠病学。