Detection of cytokines in brain lesims of humans and animal model.

我们分析了3例亚急性硬化性全脑炎（SSPE）患者和1例进行性多灶性脑白质病（PML）患者的冷冻脑组织中IL-6、干扰素- γ、肿瘤坏死因子（TNF）和转化生长因子(TGF) - β的存在。我们在SSPE脑病变中检测到这些细胞因子，表现为广泛的细胞浸润、脱髓鞘和胶质瘤。此外，我们还检测了IL-6、tnf - α、ifn - γ和tgf - β在PML患者脑病变中检测到的JC病毒抗原。这些结果表明，IL-6、il -1 β、ifn - γ、tnf - α和tgf - β可能在SSPE和PML病变中产生，并可能参与SSPE的病变发病机制（Nagano I et al .: Neurology 1994; 44: 710-715）。我们还报道了tnf - α阳性细胞分别占12例htlv -1相关脊髓病患者中6例脑脊液细胞总数的1.6-18%和8.2-23.5%，以及从炎症病例中获得的所有样本。我们检测了16例艾滋病患者和10例HIV-1血清学对照者腰骶背根神经节（DRGs）中人类免疫缺陷病毒1型（HIV-1）的免疫病理学和表达。因此，我们得出结论，HIV-1的低水平复制，通过细胞毒性T淋巴细胞或细胞因子的表达，可能在艾滋病患者DRGs中常见的感觉神经元亚临床变性中发挥作用。

We analyzed frozen brain tissues from three patients with subacute sclerosing panencephalitis (SSPE) and a patient with progressive multifocal leukoencephalopathy (PML) for the presence of IL-6, interferon-gamma, tumor necrosis factor (TNF) and transforming grwoth factor (TGF) -beta. We detected these cytokines in SSPE brain lesions showing extensive cellular infiltrates, demyelination, and gliosis. Moreover, we also detected IL-6, TNF-alpha, IFN-gamma and TGF-beta in brain lesions detected JC virus antigens from a patient with PML.These results indicate that IL-6, IL-1beta, IFN-gamma, TNF-alpha, and TGF-beta may be produced in SSPE and PML lesions, and may be involoved in the lesion pathogenesis of SSPE (Nagano I et al : Neurology 1994 ; 44 : 710-715).We also reported that TNF-alpha-positive cells constituted 1.6-18% and 8.2-23.5% of the total number of cerebrospinal fluid cells from six of 12 patients with HTLV-1-associated myelopathy and in all samples obtained from inflammatory cases, respectively.We examined the immunopathology and the expression of human immunodeficiency virus type 1 (HIV-1) in lumbosacral dorsal root ganglia (DRGs) from 16 patients with AIDS and 10 HIV-1-serological controls. Therefore, we concluded that low levels of replication of HIV-1, through cytotoxic T lymphocytes or expression of cytokines, may play a role in the subclinical degeneration of sensory neurons frequently observed in DRGs of AIDS patients.