Detection of cytokines in brain lesims of humans and animal model.

人类和动物模型脑损伤中细胞因子的检测。

• 批准号: 05670544
• 负责人: NAKAMURA Shozo
• 金额: $ 1.41万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670544/
• 关键词: cytokine; immunocytochemistry; SSPE; PML; HAM; AIDS; Cytokine; Immnohistochemistry; immunohistochemistry

We analyzed frozen brain tissues from three patients with subacute sclerosing panencephalitis (SSPE) and a patient with progressive multifocal leukoencephalopathy (PML) for the presence of IL-6, interferon-gamma, tumor necrosis factor (TNF) and transforming grwoth factor (TGF) -beta. We detected these cytokines in SSPE brain lesions showing extensive cellular infiltrates, demyelination, and gliosis. Moreover, we also detected IL-6, TNF-alpha, IFN-gamma and TGF-beta in brain lesions detected JC virus antigens from a patient with PML.These results indicate that IL-6, IL-1beta, IFN-gamma, TNF-alpha, and TGF-beta may be produced in SSPE and PML lesions, and may be involoved in the lesion pathogenesis of SSPE (Nagano I et al : Neurology 1994 ; 44 : 710-715).We also reported that TNF-alpha-positive cells constituted 1.6-18% and 8.2-23.5% of the total number of cerebrospinal fluid cells from six of 12 patients with HTLV-1-associated myelopathy and in all samples obtained from inflammatory cases, respectively.We examined the immunopathology and the expression of human immunodeficiency virus type 1 (HIV-1) in lumbosacral dorsal root ganglia (DRGs) from 16 patients with AIDS and 10 HIV-1-serological controls. Therefore, we concluded that low levels of replication of HIV-1, through cytotoxic T lymphocytes or expression of cytokines, may play a role in the subclinical degeneration of sensory neurons frequently observed in DRGs of AIDS patients.

我们分析了三名亚急性硬化性全脑炎 (SSPE) 患者和一名进行性多灶性白质脑病 (PML) 患者的冰冻脑组织中是否存在 IL-6、干扰素-γ、肿瘤坏死因子 (TNF) 和转化生长因子 (TGF)-β。我们在 SSPE 脑损伤中检测到这些细胞因子，显示出广泛的细胞浸润、脱髓鞘和神经胶质增生。此外，我们还在PML患者的脑病灶中检测到了JC病毒抗原，检测到了IL-6、TNF-α、IFN-γ和TGF-β。这些结果表明IL-6、IL-1β、IFN-γ、TNF-α和TGF-β可能在SSPE和PML病灶中产生，并且可能参与病灶的形成。 SSPE 的发病机制（Nagano I 等人：Neurology 1994；44：710-715）。我们还报道，TNF-α 阳性细胞分别占 12 名 HTLV-1 相关脊髓病患者中的 6 名以及从炎症病例获得的所有样本中的脑脊液细胞总数的 1.6-18% 和 8.2-23.5%。我们检查了的 免疫病理学以及 16 名 AIDS 患者和 10 名 HIV-1 血清学对照的腰骶背根神经节 (DRG) 中人类免疫缺陷病毒 1 型 (HIV-1) 的表达。因此，我们得出结论，通过细胞毒性T淋巴细胞或细胞因子的表达，HIV-1的低水平复制可能在艾滋病患者背根神经节中经常观察到的感觉神经元亚临床变性中发挥作用。

项目成果

Nagano I.et al: "Expression of cytokines in brain lesions in subacntic sclerosing panencephalitis" Neurology. 44. 710-715 (1994)

Nagano I.等人：“细胞因子在皮下硬化性全脑炎脑损伤中的表达”神经病学。

Nakamura S et al: "Detection of tumor necrosis factor-alpha-positive cells in CSF of patients with HAM." J Neuroimmunol. 42. 127-130 (1993)

Nakamura S 等人：“HAM 患者脑脊液中肿瘤坏死因子 α 阳性细胞的检测”。

中村 正三 他: "脊髄症状を呈した髄膜癌腫症の1例-浸潤腫瘍細胞のTNF-αの検出" 臨床神経. 33. 182-186 (1993)

Shozo Nakamura 等人：“伴有脊髓症状的脑膜癌病病例 - 浸润性肿瘤细胞中 TNF-α 的检测”《临床神经病学》，33. 182-186 (1993)。

Nakamura S.et al: "Detection of TNF-α positive tuma cells in patients with leptomeningeal carcimatosis" Acta Neurologica Scand.in press.

Nakamura S. 等人：“软脑膜癌患者中 TNF-α 阳性肿瘤细胞的检测”Acta Neurologica Scand.in press。

Nagano I.et al: "Expression of Cytokines in brain lesions in subacute sclerosing panencephalitis" Neurology. 44. 710-715 (1994)

Nagano I.等人：“亚急性硬化性全脑炎脑损伤中细胞因子的表达”神经病学。