Serum anti-phospholipid antibodies in patients with multiple sclerosis

本文应用酶联免疫吸附法检测了32例多发性硬化（MS）患者血清抗磷脂抗体（aPL）水平，并探讨了其与MS的相关性（疾病对照. A）、8名胶原血管病患者（疾病对照.B）和26名健康人（正常对照）。在MS组中，3例（9%）检测到抗心磷脂（CL）IgG抗体; 2例抗CL抗体显示辅因子（2-糖蛋白I）依赖性，但1例不依赖于辅因子。32例MS患者中有14例（449%）IgM抗体升高，但未确定辅因子依赖性。但与疾病对照组A（P<0.01）和正常对照组A（P<0.01）相比，差异有统计学意义。4例患者抗CL IgG抗体与DNA的反应性不同，APLA阳性组抗核抗体等自身抗体阳性率高于阴性组。然而，临床上两组MS是无法区分的。APLA的较高发生率意味着MS中可能产生广谱自身抗体;一些可能与MS发病机制直接相关的抗体尚未确定，但APLA可能是用于检测中枢炎症和脱髓鞘能力的抗体之一。

We have measured serum antiphospholipid antibodies (aPL) in patients with multiple sclerosis (MS) using enzyme linked immunosorbent assey and examined the correlations between these antibodies and MS.This study included thirty-two patients with clinically definite MS,thirteen patients with other autoimmune neurological disease excluding collagen vascular diseases (disease control.A) , eight patients with collagen vascular diseases (disease control.B) and twenty-six healthy persons (normal control) . In MS group IgG antibody against cardiolipin (CL) was detected in 3 (9%) ; anti-CL antibody showed cofactor (2-glycoprotein I) dependency in 2 but one was cofactor independent. IgM antibody was elevated in 14 of 32 patients (449%) with MS,but cofactor dependency was not determined. However, this was significantly higer in frequecy than that of the disease control A (P<0.01) and normal control (P<0.01) . Each anti-CL IgG antibody purified from four patients had differed reactivities against DNA.In addition, the APLA positive group in MS possessed at higer rate of other autoantibodies such as antinuclear antibody than the negative group. However, clinically two groups of MS were indistinguishable. The higher incidence of APLA imply that a broad spectrum of autoantibodies might be produced in MS ; some antibodies presumably related directly to MS pathogenesis are yet to be identified but APLA might be one of those antibodies which are to be tested for the capability of central inflammation and demyelination.