Role of calcium overload on coronary arterial stunning caused by myocardial ischemia reperfusion

钙超载对心肌缺血再灌注引起的冠状动脉顿抑的作用

• 批准号: 05670613
• 负责人: HORI Masatsugu
• 金额: $ 1.41万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670613/
• 关键词: Stunning; Autoregulation; Adenosine; Ischemia; Ca^<++>-overload; ischewia・reperfusion; corcnary autoregulation; sturning; Ca^<++> overload; 虚血・再潅流障害; 冠血管; Ca過負荷; 冠予備能

Transient myocardial ischemia attenuates pharmacological coronary vasodilation, suggesting attenuation of colonary blood flow(CBF) during reduction of coronary perfusion pressure after transient ischemia. To test this idea, 32 dogs were studied. CBF of the left anterior descending coronary artery was measured during stepwise reduction of coronary perfusion pressure by 10mmHg. In the control, reductions of CBF,fractional shortening(FS) and lactate extraction ratio (LER) were not observed during coronary perfusion pressure=100-70mmHg. Thirty min after 10 min of coronary occlusion, CBF response to adenosine (2mug/kg/min, ic) and papaverine (60mug/kg/min, ic) were attenuated by 34<plus-minus>9 and 26<plus-minus>7%, respectively, although FS,LER and basal CBF returned to control. During reduction of coronary perfusion pressure between 80-50mmHg, CBF and End/Epi flow ratio decreased by 9<plus-minus>5 and 35<plus-minus>9%, respectively, compared with the pre-ischemic condition. Both FS and LER were attenuated (p<.01) at coronary perfusion pressure=80-50mmHg (FS : 9.8<plus-minus>1.2 vs.20.6<plus-minus>1.2%, LER : -18.5<plus-minus>6.8 vs.26.7<plus-minus>3.6% at coronary perfusion pressure=70mmHg). Thus we conclude that transient ischemia mainly impairs autoregulation of endomyocadial flow, and causes myocardial contractile and metabolic dysfunction when coronary perfusion pres

短暂性心肌缺血可减弱药物性冠状动脉舒张作用，提示短暂性心肌缺血后冠状动脉灌注压降低时结肠血流量（CBF）减弱。为了验证这个想法，研究人员对32只狗进行了研究。在冠状动脉灌注压逐步降低10 mmHg的过程中，测量冠状动脉左前降支的CBF。对照组在冠脉灌注压100- 70 mmHg范围内，CBF、短轴缩短率（FS）和乳酸提取率（LER）均无明显降低。冠状动脉闭塞10分钟后30分钟，腺苷（2 μ g/kg/min，ic）和罂粟碱（60 μ g/kg/min，ic）对CBF的反应分别减弱了<plus-minus>34.9%和26.7%<plus-minus>，尽管FS、LER和基础CBF恢复到对照水平。冠脉灌注压降低至80- 50 mmHg时，CBF和End/Epi血流比分别较缺血前下降<plus-minus>9.5%和<plus-minus>35.9%。在冠状动脉灌注压=80- 50 mmHg时，FS和LER均减弱（p&lt;0.01）（FS：9.8 <plus-minus>± 1.2 vs.20.6 <plus-minus>± 1.2%，LER：-18.5 <plus-minus>± 6.8 vs.26.7 ± <plus-minus>3.6%，冠状动脉灌注压= 70 mmHg）。因此，我们认为短暂性缺血主要损害了心肌内膜流量的自动调节，当冠状动脉灌注加压时，

项目成果

Masatugu Hori,Masafumi Kitakaze,et al.: "The beneficial roles of alpha-adrenoceptor activity in myocardial ischemic and reperfusion injury.In Recent Advances in Coronary Circulation" Springer-Verlag,Tokyo, 17 (1993)

Masatugu Hori、Masafumi Kitakaze 等人：“α-肾上腺素受体活性在心肌缺血和再灌注损伤中的有益作用。冠状动脉循环的最新进展”Springer-Verlag，东京，17 (1993)

Masafumi Kitakze,Tetsuo Minamino,et al.: "Role of coronary perfusion on myocardial contractility in the heart.In Cardiac Failure and Adaptation" Springer-Verlag,Tokyo,

Masafumi Kitakze、Tetsuo Minamino 等人：“冠状动脉灌注对心脏心肌收缩力的作用。心力衰竭和适应”Springer-Verlag，东京，

Masafumi Kitakaze,Masatsugu Hori,et al.: "Alpha_1-adrenoceptor activation increases ectosolic 5°-nucleotidase activity and adenosine release in rat cardiomyocytes by activing protein kinase C." Circulation.

Masafumi Kitakaze、Masatsugu Hori 等人：“Alpha_1-肾上腺素受体激活通过激活蛋白激酶 C 增加大鼠心肌细胞内的 5°-核苷酸酶活性和腺苷释放。”

Tetsuo Minamino,Masafumi Kitakaze,et al.: "Bidirectional adverse effects of aminophylline on myocardial ischemia:Merit due to stimulation of alpha1-adrenergic receptors and demerit due to blockade of adenosine receptor." Ciculation.

Tetsuo Minamino、Masafumi Kitakaze 等人：“氨茶碱对心肌缺血的双向不良作用：由于刺激 α1 肾上腺素受体而优点，由于阻断腺苷受体而缺点。”

Masafumi Kitakaze,Tetsuo Minamino,et al.: "Beneficial effects of inhibition of angiotensin converting enzyme on ischemic myocardium during coronary hypoperfusion in dogs." Circulation. (in press).

Masafumi Kitakaze、Tetsuo Minamino 等人：“抑制血管紧张素转换酶对狗冠状动脉低灌注期间缺血性心肌的有益作用。”