Estimation of acute and chronic hypoxic cell fraction in experimental tumor using fluorescence dye.

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We investigated changes in blood in normal and SCC-7 tumors treated by hyperthermia combined with hydralazine to evaluate the enhancement of thermal tumor damage by hydralazine. We studied DCC-7 tumor-bearing C3H/He mice. Hydralazine was administrated by intraperitoneal injection, and tumor was heated by a water bath. We measured blood flow using the laser Doppler method, and oxygen tension using polarography. The response of tumors to therapy was assessed using a growth delay analyze. In tumor, blood flow and O2 tension significantly decreased with increased doses of hydralazine. Compared to tumor treated by hydralazine alone or by hyperthermia alone, tumor blood flow was significantly decreased in the group treated by hyperthermia with hydralazine, blood flow was significantly decreased with Hyd dose, heat during, and temperatures. In normal muscle, hyperthermia, hydralazine or their combination induced no decreased in blood flow. In tumor treated by hyperthermia (43C, 20min) with hydralazine, maximum additional growth delay was observed. Our results that a decreased in tumor blood flow caused by hydralazine play an important role in enhancement of hyperthermic anti-tumor effects.