Estimation of acute and chronic hypoxic cell fraction in experimental tumor using fluorescence dye.

我们研究了正常和经热疗联合肼治疗的SCC-7肿瘤的血液变化，以评价肼对热肿瘤损伤的增强作用。我们以DCC-7荷瘤C3H/He小鼠为研究对象。肼丙嗪腹腔注射，水浴加热肿瘤。我们用激光多普勒法测量血流量，用极谱法测氧分压。用生长延迟分析评估肿瘤对治疗的反应。在肿瘤中，血流量和氧分压随肼丙嗪剂量的增加而显著降低。与单纯热疗组和单纯热疗组相比，热疗组肿瘤血流量明显减少，且随着热疗剂量、时间和温度的增加，肿瘤血流量显著减少。在正常肌肉中，高温、肼丙嗪或两者合用均可引起血流量下降。在热疗(43℃，20min)联合肼的肿瘤中，观察到最大的附加生长延迟。我们的研究结果表明，呋喃西林引起的肿瘤血流量减少在增强热疗抗肿瘤效应中起着重要作用。

We investigated changes in blood in normal and SCC-7 tumors treated by hyperthermia combined with hydralazine to evaluate the enhancement of thermal tumor damage by hydralazine. We studied DCC-7 tumor-bearing C3H/He mice. Hydralazine was administrated by intraperitoneal injection, and tumor was heated by a water bath. We measured blood flow using the laser Doppler method, and oxygen tension using polarography. The response of tumors to therapy was assessed using a growth delay analyze. In tumor, blood flow and O2 tension significantly decreased with increased doses of hydralazine. Compared to tumor treated by hydralazine alone or by hyperthermia alone, tumor blood flow was significantly decreased in the group treated by hyperthermia with hydralazine, blood flow was significantly decreased with Hyd dose, heat during, and temperatures. In normal muscle, hyperthermia, hydralazine or their combination induced no decreased in blood flow. In tumor treated by hyperthermia (43C, 20min) with hydralazine, maximum additional growth delay was observed. Our results that a decreased in tumor blood flow caused by hydralazine play an important role in enhancement of hyperthermic anti-tumor effects.