Estimation of acute and chronic hypoxic cell fraction in experimental tumor using fluorescence dye.

我们研究了热疗联合肼嗪治疗的正常和SCC-7肿瘤的血液变化，以评估肼嗪对热肿瘤损伤的增强作用。我们研究了DCC-7荷瘤C3H/He小鼠。腹腔注射肼嗪，水浴加热肿瘤。我们用激光多普勒法测量血流，用极谱法测量氧张力。肿瘤对治疗的反应用生长延迟分析来评估。在肿瘤中，血流量和氧张力随着肼嗪剂量的增加而显著降低。与单用肼嗪治疗组和单用热疗组比较，肼嗪热疗组肿瘤血流量明显减少，血流量随剂量、热疗期间和温度的升高而明显减少。在正常肌肉中，热疗、肼嗪或两者联合均未引起血流量减少。在用肼热疗（43C, 20min）治疗的肿瘤中，观察到最大的额外生长延迟。我们的研究结果表明，肼嗪引起的肿瘤血流量减少在增强热疗抗肿瘤作用中起重要作用。

We investigated changes in blood in normal and SCC-7 tumors treated by hyperthermia combined with hydralazine to evaluate the enhancement of thermal tumor damage by hydralazine. We studied DCC-7 tumor-bearing C3H/He mice. Hydralazine was administrated by intraperitoneal injection, and tumor was heated by a water bath. We measured blood flow using the laser Doppler method, and oxygen tension using polarography. The response of tumors to therapy was assessed using a growth delay analyze. In tumor, blood flow and O2 tension significantly decreased with increased doses of hydralazine. Compared to tumor treated by hydralazine alone or by hyperthermia alone, tumor blood flow was significantly decreased in the group treated by hyperthermia with hydralazine, blood flow was significantly decreased with Hyd dose, heat during, and temperatures. In normal muscle, hyperthermia, hydralazine or their combination induced no decreased in blood flow. In tumor treated by hyperthermia (43C, 20min) with hydralazine, maximum additional growth delay was observed. Our results that a decreased in tumor blood flow caused by hydralazine play an important role in enhancement of hyperthermic anti-tumor effects.