Estimation of acute and chronic hypoxic cell fraction in experimental tumor using fluorescence dye.

使用荧光染料估计实验肿瘤中急性和慢性缺氧细胞分数。

• 批准号: 05670791
• 负责人: AKAGI Kiyoshi
• 金额: $ 1.34万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670791/
• 关键词: Experimental tumor; Oxygen tension; Blood flow; 腫瘍組織; 低酸素細胞分画; 蛍光色素; 放射線抵抗性; 放射線照射; Flow sytometry; 低酸素細胞

We investigated changes in blood in normal and SCC-7 tumors treated by hyperthermia combined with hydralazine to evaluate the enhancement of thermal tumor damage by hydralazine. We studied DCC-7 tumor-bearing C3H/He mice. Hydralazine was administrated by intraperitoneal injection, and tumor was heated by a water bath. We measured blood flow using the laser Doppler method, and oxygen tension using polarography. The response of tumors to therapy was assessed using a growth delay analyze. In tumor, blood flow and O2 tension significantly decreased with increased doses of hydralazine. Compared to tumor treated by hydralazine alone or by hyperthermia alone, tumor blood flow was significantly decreased in the group treated by hyperthermia with hydralazine, blood flow was significantly decreased with Hyd dose, heat during, and temperatures. In normal muscle, hyperthermia, hydralazine or their combination induced no decreased in blood flow. In tumor treated by hyperthermia (43C, 20min) with hydralazine, maximum additional growth delay was observed. Our results that a decreased in tumor blood flow caused by hydralazine play an important role in enhancement of hyperthermic anti-tumor effects.

我们研究了热疗联合肼嗪治疗的正常和SCC-7肿瘤的血液变化，以评估肼嗪对热肿瘤损伤的增强作用。我们研究了DCC-7荷瘤C3H/He小鼠。腹腔注射肼嗪，水浴加热肿瘤。我们用激光多普勒法测量血流，用极谱法测量氧张力。肿瘤对治疗的反应用生长延迟分析来评估。在肿瘤中，血流量和氧张力随着肼嗪剂量的增加而显著降低。与单用肼嗪治疗组和单用热疗组比较，肼嗪热疗组肿瘤血流量明显减少，血流量随剂量、热疗期间和温度的升高而明显减少。在正常肌肉中，热疗、肼嗪或两者联合均未引起血流量减少。在用肼热疗（43C, 20min）治疗的肿瘤中，观察到最大的额外生长延迟。我们的研究结果表明，肼嗪引起的肿瘤血流量减少在增强热疗抗肿瘤作用中起重要作用。

项目成果

Yoshizumi Aoki: "Enhancement of Thermal damage in murine tumors by hydralazion-induced modificaTion of blood flow and oxygen tension" Int.J.of Oncology. 11. 1123-1128 (1997)

Yoshizumi Aoki：“通过肼苯哒嗪诱导的血流和氧张力的改变来增强小鼠肿瘤的热损伤”Int.J.of Oncology。

Yoshizumi Aoki, Hiroyoshi Isoda, Taichi Oshima, Kiyoshi Akagi, Toshiko Shiga, Reiko Nasu, Takashi Mrata, Yoshimasa Tanaka: "Enhancement of thermal damage in murine tumors by hydralazine-induced modification of blood flow and oxygen tension" Int.J.of Oncol

Yoshizumi Aoki、Hiroyoshi Isoda、Taichi Oshima、Kiyoshi Akagi、Toshiko Shiga、Reiko Nasu、Takashi Mrata、Yoshimasa Tanaka：“肼苯哒嗪诱导的血流和氧张力改变增强小鼠肿瘤的热损伤” Int.J.of Oncol

Yoshizumi Aoki, Kiyoshi Akagi, Yoshimasa Tanaka, Jun Kawai, Masaya Takahashi: "Measurement of intratumor pH by pH indicator used in 19F-magnetic resonance spectroscopy -Measurement of extra cellular pH decrease caused by hyperthermia combined with hydrala

Yoshizumi Aoki、Kiyoshi Akagi、Yoshimasa Tanaka、Jun Kawai、Masaya Takahashi：“通过 19F 磁共振波谱中使用的 pH 指示剂测量肿瘤内 pH -测量热疗与水螅结合引起的细胞外 pH 值降低

Yoshizumi Aoki: "Measurement of intratumor pH by pH indicator used in 19F-magnetic resonance spectroscopy-Measurement of extra cellular pH decrease caused by heperThermia combined with hydralazine" Investigative Radiology. 31. 680-689 (1996)

Yoshizumi Aoki：“通过 19F 磁共振波谱法中使用的 pH 指示剂测量肿瘤内 pH - 测量热疗与肼苯哒嗪联合引起的细胞外 pH 值降低”调查放射学。

青木 良純: "実験腫瘍における温熱とヒドララジンの併用による31P-MRSおよび組織血流からの抗腫瘍効果の検討" 日本医学放射線学会雑誌. 55. 987-992 (1995)

Yoshizumi Aoki：“在实验性肿瘤中联合使用热疗和肼屈嗪的 31P-MRS 和组织血流的抗肿瘤效果的检查”日本医学放射学会杂志 55. 987-992 (1995)。