Cell kinetics in cultured tumor cells aftertreatment with radiation and chemotherapy

放疗和化疗后培养的肿瘤细胞的细胞动力学

• 批准号: 07671034
• 负责人: AKAGI Kiyoshi
• 金额: $ 1.34万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671034/
• 关键词: Irradiation; Experimental Tumor; Cell Cycle; アポトーシス; 放射線; 抗Fas抗体; 照射効果

The cell cycle was analyzed using double staining with an anti-bromodeoxyuridine (BrDU) monoclonal anti-body and propidium iodide (PI). Changes in cell kinetics after irradiation were compared with those seen by the conventional PI-based DNA histogram method. The effect of irradiation on cell kinetics has been studied primarily by counting G2 arrested cells. By the present BrdU method, a rapid tradition, from G1 to S-phase was observed within 2h of irradiation, followed by G1 blook. Cell in the S-phase progressed to G2+M where they arrested, resulting in a decreased percentage of S cells, (<5%). Release of G1 after>18h. The initial Glarrest induced by irradiation was confirmed for the first time by the present BrdU-PI double atsining.

用抗溴脱氧尿苷（BrDU）单克隆抗体和碘化丙啶（PI）双重染色分析细胞周期。照射后的细胞动力学的变化进行了比较，与传统的PI为基础的DNA直方图方法所看到的。辐射对细胞动力学的影响主要通过计数G2期阻滞细胞来研究。通过本BrdU方法，在照射后2小时内观察到从G1期到S期的快速传统，然后是G1期阻滞。处于S期的细胞进展到G2+M，在那里它们停滞，导致S细胞百分比降低（<5%）。> 18 h后释放G1。首次用BrdU-PI双标记法证实了辐射诱导的初始Glarrest。

项目成果

Murata Takashi: "Analysis of cell kineTics after gamma ray irradiation using anti-BrdU monoclonal antibody" Int.J.Oncology. 12. 345-349 (1998)

Murata Takashi：“使用抗 BrdU 单克隆抗体分析伽马射线照射后的细胞动力学”Int.J.Oncology。

Takashi Murata: "Cell kinetics changes in cultured tumor cells after treatment with radiation and chemotherapy" Int.J.Oncology. 12. 171-174 (1998)

Takashi Murata：“放射和化疗治疗后培养的肿瘤细胞的细胞动力学变化”Int.J.Oncology。

Hiroyuki Isoda, Kiyoshi Akagi, Takeo Hoosegow, Yoshimasa Tanaka, T.Kihara, S.Sakata, M.Ikeda: "Detection of an increase in ascorbate radical in an irradiated experimental tumour system using ESR" Int.J.Radiat. Biol.68. 467-473 (1995)

Hiroyuki Isoda、Kiyoshi Akagi、Takeo Hoosegow、Yoshimasa Tanaka、T.Kihara、S.Sakata、M.Ikeda：“使用 ESR 检测辐照实验肿瘤系统中抗坏血酸自由基的增加”Int.J.Radiat。