Cell kinetics in cultured tumor cells aftertreatment with radiation and chemotherapy

点击翻译按钮获取中文摘要

The cell cycle was analyzed using double staining with an anti-bromodeoxyuridine (BrDU) monoclonal anti-body and propidium iodide (PI). Changes in cell kinetics after irradiation were compared with those seen by the conventional PI-based DNA histogram method. The effect of irradiation on cell kinetics has been studied primarily by counting G2 arrested cells. By the present BrdU method, a rapid tradition, from G1 to S-phase was observed within 2h of irradiation, followed by G1 blook. Cell in the S-phase progressed to G2+M where they arrested, resulting in a decreased percentage of S cells, (<5%). Release of G1 after>18h. The initial Glarrest induced by irradiation was confirmed for the first time by the present BrdU-PI double atsining.