The cDNA clones which is similar to the LDL-receptor were selected by hybridization.

通过杂交选择与LDL受体相似的cDNA克隆。

• 批准号: 05670841
• 负责人: SAITO Yasushi
• 金额: $ 1.34万
• 依托单位: Yamagata University (1994)Chiba University (1993)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670841/
• 关键词: LDL-receptor; LDL; Binding domain; EGF; transmembrance; 動脈硬化; 脂質代謝; クローニング

The cDNA clones which is similar to the LDL-receptor were selected by hybridization.The probes were used as follows. One is the LDL-receptor binding domain and the other has no homology to the LDL-receptor. Using these two probes, we selected several clones and finally, we selected the clone which is homologous to the LDL-receptor but not LDL-receptor (LDL-receptor homologous protein LRHP).The DNA sequence was determined. It has 6962 pb.Amino acid sequence has the EGF precursor domain, LDL binding domain and tramsmembrane domain. The amino acid sequence similarity to the known protein not detected. The function and its significance are now in progress.

通过杂交选择与LDL受体相似的cDNA克隆。一个是LDL受体结合结构域，另一个与LDL受体没有同源性。利用这两种探针，我们选择了几个克隆，最后，我们选择了一个与LDL-受体同源但与LDL-受体无关的克隆（LDL-受体同源蛋白LRHP）。氨基酸序列包括EGF前体结构域、LDL结合结构域和跨膜结构域。未检测到与已知蛋白的氨基酸序列相似性。其功能和意义正在进行中。

项目成果

I.Ishii, T.Kimuro, U.Saito, S.Hirose: "Cholesterol metabolism in monocyte-derived macrophages from macrophage colony-stimulating factor administered rabbit" Biochimica et Biophysica Acta.1254. 51-55 (1995)

I.Ishii、T.Kimuro、U.Saito、S.Hirose：“给予巨噬细胞集落刺激因子的兔子的单核细胞衍生巨噬细胞中的胆固醇代谢”Biochimica et Biophysicala Acta.1254。

F.Kobayashi, Y.Saito, S.Yoshida: "Hypertriglyceridemia in cardiovascular and metabolic diseases : familial dyslipidemic hypertension and coronary heart disease" Triglycerides and atherosclerosis. 125-134 (1994)

F.Kobayashi、Y.Saito、S.Yoshida：“心血管和代谢疾病中的高甘油三酯血症：家族性血脂异常高血压和冠心病”甘油三酯和动脉粥样硬化。

N.Morisaki,H.Inadera,Y.Saito,S.Yoshida,K.Shigemura,et.al.: "Diabetic Control and Progression of Retinopathy in Elderly Patients:Five-Year Follow-up Study" JAGS. 42. 142-145 (1994)

N.Morisaki、H.Inadera、Y.Saito、S.Yoshida、K.Shigemura 等人：“老年患者糖尿病控制和视网膜病变进展：五年随访研究”JAGS。

I.Ishii,T.Kimuro,Y.Saito,S.Hirose.: "Cholesterol metabolism in monocyte-derived macrophages from macrophage colony-stimulating factor administered rabbits" Biochimica et Biophysica Acta. 1254. 51-55 (1995)

I.Ishii，T.Kimuro，Y.Saito，S.Hirose.：“给予巨噬细胞集落刺激因子的兔子的单核细胞衍生的巨噬细胞中的胆固醇代谢”Biochimica et Biophysicala Acta。

J.Kobayashi, Y.Saito, K.Niihara, N.Sasaki, .et.al.: "Effects of Treatment with Bezafibrate on Lipoprotein Lipase Activity and Mass in Patients with Hypertriglyceridemia" Arzneimittel-Forschung Drug Research. 44. 145-148 (1994)

J.Kobayashi、Y.Saito、K.Niihara、N.Sasaki 等人：“苯扎贝特治疗对高甘油三酯血症患者脂蛋白脂肪酶活性和质量的影响”Arzneimittel-Forschung 药物研究。