Research for the Regulation Factor which is Produced from the Endothelial Cell Depend on its Cell Cycle and Acts on the Proliferation and Metabolism.

内皮细胞产生的调节因子依赖于其细胞周期并作用于增殖和代谢的研究。

• 批准号: 63570281
• 负责人: SAITO Yasushi
• 金额: $ 1.34万
• 依托单位: CHIBA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570281/
• 关键词: Endothelial Cell; Smooth Muscle Cell; Phenotype; Growth Factors; Phenotype; 増殖因子; 中膜平滑筋細胞; マクロファージ; 低比重リポ蛋白

The aim of this project is to clarify the factor to produce the intimal smooth muscle cell (SMC) because the intimal thickening, which is constantly observed in atheromatous lesions, is mainly consist of the intimal SMC. It is generally accepted that intimal SMC might be derived from the inedial SMC by migration and the SMC is enhanced to grow by some growth factors. In these process, the endothelial cell is considered to play an important role. Therefore, we tried to examined the effect of endothelial cell (EC) on the SMC growth using the EC-SMC co-culture system. The system was established and the results are as follows.1) Co-cultured SMC growth with EC was inhibited but slightly increased in the presence of Iridoinethasine. 2) Conditioned medium with EC enhanced the SMC growth in the growing phase more than in the confluent phase. 3) The SMC secreted the SDGF (smooth muscle derived growth factor) when the SMC was cultured with the conditioned medium. 4) The growth stimulating activity from the EC was inhibited by the addition of anti-PDGF antibody. 5) PDGF itself stimulates the SDGF secretion from SMC.Above these results, we concluded that EC modulated the SMC phenotype and one of the factors from EC to change the SMC phenotype might be PDGF.

由于动脉粥样硬化病变中经常观察到的内膜增厚主要由内膜平滑肌细胞（SMC）组成，因此本项目的目的是阐明产生内膜平滑肌细胞（SMC）的因素。一般认为，内膜SMC可能是由内膜SMC迁移而来，某些生长因子可促进SMC生长。在这些过程中，内皮细胞被认为起着重要的作用。因此，我们尝试用内皮细胞（EC）与平滑肌细胞（SMC）共培养系统来研究EC对SMC生长的影响。结果表明：1）环烯醚萜对与EC共培养的SMC的生长有抑制作用，但对SMC的生长有促进作用。2)EC条件培养基对生长期SMC生长的促进作用大于汇合期。3)当SMC与条件培养基一起培养时，SMC分泌SDGF（平滑肌衍生生长因子）。4)加入抗PDGF抗体可抑制EC的生长刺激活性。5)上述结果表明，EC对SMC表型有调节作用，而PDGF可能是EC改变SMC表型的因素之一。

项目成果

Saito Y, Shinomiya M, Shirai K, Yoshida S: "Treatment of Severe Hypercholesterolemia by LDL-Apheresis: Cholesterol-Lowering Effect and Clinical Evaluation." Contributions to Infusion Therapy 23: 160-171, 1988.

Saito Y、Shinomiya M、Shirai K、Yoshida S：“通过 LDL-Apheresis 治疗严重高胆固醇血症：降胆固醇效果和临床评估。”

Y.Saito;H.Bujo;N.Morisaki;K.Shirai;S.Yoshida: Atherosclerosis. 69. 161-164 (1988)

Y.Saito；H.Bujo；N.Morisaki；K.Shirai；S.Yoshida：动脉粥样硬化。

J.Kobayashi;T.Niside;M.Shinomiya;N.Sasaki;K.Shirai;Y.Saito;S.Yoshida: Atherosclerosis. 73. 105-111 (1988)

J.Kobayashi；T.Niside；M.Shinomiya；N.Sasaki；K.Shirai；Y.Saito；S.Yoshida：动脉粥样硬化。

N.Morisaki;T.Kanzaki;Y.Saito;S.Yoshida: Atherosclerosis. 73. 67-69 (1988)

N.Morisaki；T.神崎；Y.Saito；S.Yoshida：动脉粥样硬化。

Saito Y, Kanzaki T, Morisaki N, Koshikawa T & Yoshida S: "Modification of Growth Property of Aortic Smooth Muscle Cells by the Endothelial Cells." Clinica & Terapia Cardiovascolare VIII(3): 261-264, 1989.

齐藤 Y、神崎 T、森崎 N、越川 T