Molecular analysis of thyrotropin receptor in thyroid noeplasm

甲状腺肿瘤中促甲状腺素受体的分子分析

• 批准号: 05670850
• 负责人: ENDO Toyoshi
• 金额: $ 1.41万
• 依托单位: University of Yamanashi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670850/
• 关键词: thyrotropin; thyrotropin receptor; point mutation; neoplasm; thyroid; サイロトロピン・レセプター(TSH-R); cDNA; 変異

Total RNA was prepared from 34 thyroid tisssue samples (four normal, six adenoma, six follicular cancer, and 18 papillary cancer) and reverse-transcribed into single-stranded cDNA,which was then used as a templated for the polymerase chain reaction and subjected to single-strand conformation polymorphism (SSCP) analysis. Two fragments, FRAG (468-692) (nucleotides 2044 to 2295, corresponding to the COOH-terminal, cytoplasmic dmain of the TSH-R cDNA) showed differences in electrophoretic mobility among the various thyroid tissue samples. Direct sequencing revealed Phe^<197> (TTC) * Ile (ATC), and Asp^<219> (GAT) * Glu (GAG) substitutions in FRAG (468-692) form two papillary cancers. Three types of substitution were identified in FRAG (2044-2295) : Asn^<715> (AAC) * Asp (GAC) from one papillary cancer, Lys^<723> (AAG) * Met (ATG) from one papillary cancer, and Asp^<727> (GAC) * Glu (GAG) from one normal tissue sample, one follicular cancer, and four papillary cancers. These results suggest that there exist structure changes in TSH-R in some cases of thyroid neoplastic tissue.

从34例甲状腺组织（4例正常、6例腺瘤、6例滤泡癌和18例乳头状癌）中提取总RNA，反转录成单链cDNA，然后将其作为聚合酶链反应的模板，进行单链构象多态性（SSCP）分析。两个片段FRAG（468-692）（核苷酸2044 - 2295，对应于TSH-R cDNA的COOH-末端，胞质末端）在不同甲状腺组织样品中的电泳迁移率存在差异。直接测序显示，FRAG（468-692）中的Phe^<197>（TTC）* Ile（ATC）和Asp^<219>（GAT）* Glu（GAG）取代形成了两种乳头状癌。在FRAG（2044-2295）中鉴定了三种类型的取代：<715>来自一种乳头状癌的Asn^（AAC）* Asp（GAC），<723>来自一种乳头状癌的Lys^（AAG）* Met（ATG），以及<727>来自一种正常组织样品、一种滤泡癌和四种乳头状癌的Asp^（GAC）* Glu（GAG）。提示部分甲状腺肿瘤组织中存在TSH-R结构的改变。

项目成果

遠藤登代志: "甲状腺細胞の腫瘍化と遺伝子発現" BIO clinica. 11. 797-799 (1994)

Toyoshi Endo：“甲状腺细胞的肿瘤和基因表达”BIO 临床 11. 797-799 (1994)。

遠藤登代志 他: "甲状腺細胞の腫瘍化と遺伝子発現" BIO clinica. 11. 797-799 (1994)

Toyoshi Endo 等人：“甲状腺细胞的肿瘤和基因表达”BIO 临床 11. 797-799 (1994)。

Ohno,M,et al.: "Point mutations of thyrotropin receptor in thyroid tumor tissues" THYROID. (in press).

Ohno,M,et al.：“甲状腺肿瘤组织中促甲状腺素受体的点突变”甲状腺。

Ohno M,Endo T,Ohta K,Gunji K,Onaya T: Thyroid. 5. 97-100 (1995)

Ohno M、Endo T、Ohta K、Gunji K、Onaya T：甲状腺。

Ohno,M.et al.: "Point mutations of thyrotropin receptor in thyroid tissues." THYROID. (in press).

Ohno,M.et al.：“甲状腺组织中促甲状腺素受体的点突变。”