Organogenesis of pancreas in PDX-1 promoter/YFP transgenic mouse

PDX-1启动子/YFP转基因小鼠胰腺的器官发生

• 批准号: 15590934
• 负责人: ENDO Toyoshi
• 金额: $ 2.24万
• 依托单位: University of Yamanashi
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590934/
• 关键词: pancreatic puodenal homeobox-1; pancreas; β cell; organogenesis; pancreatic duct; 膵臓; β細胞; トランスジェニックマウス; 糖尿病; pancreatic duodenal; homeobox-1; EYFP

Pancreatic duodenal homeobox-1 (PDX-1) is the key molecule in organogenesis of the pancreas. To further investigate its roles in organogenesis of pancreas, we developed PDX-1 promoter/YFP transgenic mouse. We cloned rat PDX-1 promoter region (-6.2 Kb) and then inserted into multiple cloning site of pEYFP. Six lines of mouse were transgened and established. Development and growth of the transgenic animal were normal and blood glucose levels of those were also normal range. Morphologically, islets of those were relatively large, and number of beta cells was increased. However, the glucagons-positive cells formed a cluster and localized near the pancreatic duct, which were also hyperplastic. Focally, lymphocyte infiltration around the ducts was observed. It will be needed to analyze detail the role of the transgened cis element in those phenotypes in pancreas.

胰腺十二指肠同源异型盒-1（PDX-1）是胰腺器官发生的关键分子。为了进一步研究其在胰腺器官发生中的作用，我们建立了PDX-1启动子/YFP转基因小鼠。我们克隆了大鼠PDX-1启动子区（~ 6.2Kb），并将其插入pEYFP的多克隆位点。建立了6个转基因小鼠品系。转基因动物的发育和生长正常，血糖水平也在正常范围内。形态学上，这些胰岛相对较大，β细胞数量增加。然而，胰高血糖素阳性细胞形成一个集群，并定位于附近的胰管，这也是增生。在导管周围观察到局灶性淋巴细胞浸润。需要详细分析转基因的顺式元件在胰腺中这些表型中的作用。

项目成果

Distinct diagnostic criteria of fulminant type I diadetes based on serum C-peptide response and HbA1c levels at onset.

基于血清 C 肽反应和发病时 HbA1c 水平的暴发性 I 型糖尿病的独特诊断标准。

• 发表时间: 2004
• 期刊: Dibetes Care 27
• 作者: Tanaka S;Endo T;Aida K et al.
• 通讯作者: Aida K et al.

Distinct diagnostic criteria of fulminant type I diadetes based on serum C-peptide response and HbA1c levels at onset

基于血清 C 肽反应和发病时 HbA1c 水平的暴发性 I 型糖尿病的独特诊断标准

• 发表时间: 2004
• 期刊: Dibetes Care 27
• 作者: Tanaka S;Endo T;Aida K et al.
• 通讯作者: Aida K et al.

Distinct Diagnostic Criteria of Fuluminat Type I Diabetes Based on Serum C peptide Response and HbAic levels at Onset

基于血清 C 肽反应和发病时 HbAic 水平的 Fuluminat I 型糖尿病的独特诊断标准

• 发表时间: 2004
• 期刊: Diadetes Care 27
• 作者: Tanaka S;Endo T;Aida K et al.
• 通讯作者: Aida K et al.