Characterization of autoantibody against Amylaseα-2A in patients with fulminant type 1 diabetes mellitus and autoimmune pancreatitis, and its clinical use.

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Fulminant type 1 diabetes mellitus(FT1DM) is a subtype of T1DM, which frequently induces diabetic ketoacidosis. However, no useful marker for rapid diagnosis of FT1DM exsist. We reported that patients with FT1DM have an autoantibody against Amylaseα-2A(AMY), and developed a enzyme linked immunosorbent assay(ELISA) for detecting the antibody. Because background in the system is relatively high, so, we have improved ELISA using synthetic peptide corresponding AMY as a new antigen. When we employed peptide-7 as an antigen, we obtained good results for detecting AMY autoantibody from patients with FT1DM(sensitivity 94. 3%, specificity 93. 7% by ROC analysis), which might applicable for clinical use for rapid diagnosis of FT1DM.