Three Subunits of IL-2 Receptor

IL-2 受体的三个亚基

• 批准号: 05670984
• 负责人: SATOMI Susumu
• 金额: $ 1.34万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670984/
• 关键词: IL-2 receptor; anti-IL-2Ralpha antibody; anti-IL-2Rbeta antibody; anti-IL-2Rgamma antibody; skin allograft; 抗γ鎖抗体; MLC; IL-2の吸収; 細胞内刺激伝達抑制; 遊離IL-2受容体; 皮膚移植延長効果

The IL-2 receptor (IL-2R) gamma chain, so-called common gamma (gammac) chain, which is shared with multiple cytokine receptors, plays important roles in the immune system. We here assessed the immunosuppressive ability of mAb specific for the gammac chain in induction of CTLs and allograft rejection in combination with mAbs specific for the alpha and beta chains of IL-2R.CBA/N (H-2^k) mice were injected intraperitoneally with allogeneic splenocytes from BALB/c (H-2^d) mice, and then administered with combinations of anti-IL-2Ralpha, anti-IL-2Rbeta and anti-gammac mAbs or a control mAb. Addition of anti-gammac mAb together with anti-IL-2Ralpha and anti-IL-2Rbeta mAbs induced a complete inhibition of CTL response. The numbers and populations of CD4^+CD8^- and CD4^-CD8^+ T cells were not significantly affected by administration of the three anti-IL-2R mAbs, whereas NK cells were completely depleted in spleens of mice treated with the anti-IL-2R mAbs. Furthermore, skin allograft survival was also significantly prolonged by administration of the three anti-IL-2R mAbs. These results suggest that the anti-gammac mAb in combination with anti-IL-2Ralpha and anti-IL-2Rbeta mAbs is capable of suppressing induction of CTLs and NK cells, resulting in prolongation of skin allograft survival.

IL-2受体(IL-2R) γ链，即所谓的公共γ链，与多种细胞因子受体共享，在免疫系统中起着重要作用。我们在此评估了γ - mac链特异性单克隆抗体与IL-2R α链和β链特异性单克隆抗体联合诱导ctl和同种异体移植排斥反应的免疫抑制能力。将BALB/c （H-2^d）小鼠的同种异体脾细胞腹腔注射给CBA/N （H-2^k）小鼠，然后联合使用抗il - 2rα、抗il - 2rβ和抗γ单抗或对照单抗。抗gammac单抗与抗il - 2rα和抗il - 2rβ单抗一起添加可完全抑制CTL反应。三种抗il - 2r单抗对小鼠脾脏中CD4^+CD8^-和CD4^-CD8^+ T细胞的数量和群体没有显著影响，而抗il - 2r单抗处理小鼠脾脏中NK细胞完全消失。此外，三种抗il - 2r单抗也显著延长了同种异体皮肤移植的存活时间。这些结果表明，抗gammac单抗与抗il - 2rα和抗il - 2rbeta单抗联合使用能够抑制ctl和NK细胞的诱导，从而延长同种异体皮肤的存活时间。