Three Subunits of IL-2 Receptor

IL-2受体（IL-2 R）γ链，所谓的共同γ（gammac）链，其与多种细胞因子受体共享，在免疫系统中起重要作用。我们在此评估了γ链特异性单克隆抗体与IL-2 R α和β链特异性单克隆抗体联合诱导CTL和同种异体移植排斥反应的免疫抑制能力。CBA/N（H-2k）小鼠腹腔内注射来自BALB/c（H-2d）小鼠的同种异体脾细胞，然后给予抗IL-2 R α，抗IL-2 R β和抗γ mAb或对照mAb。抗γ mAb与抗IL-2 R α和抗IL-2 R β mAb一起加入诱导CTL应答的完全抑制。给予三种抗IL-2 R mAb对CD 4 ^+ CD 8 ^-和CD 4 ^-CD 8 ^+ T细胞的数量和群体没有显著影响，而用抗IL-2 R mAb处理的小鼠脾脏中的NK细胞完全耗尽。此外，皮肤同种异体移植物的存活也显着延长了三个抗IL-2 R单克隆抗体的管理。这些结果表明，抗γ mAb与抗IL-2 R α和抗IL-2 R β mAb的组合能够抑制CTL和NK细胞的诱导，导致皮肤同种异体移植物存活的延长。

The IL-2 receptor (IL-2R) gamma chain, so-called common gamma (gammac) chain, which is shared with multiple cytokine receptors, plays important roles in the immune system. We here assessed the immunosuppressive ability of mAb specific for the gammac chain in induction of CTLs and allograft rejection in combination with mAbs specific for the alpha and beta chains of IL-2R.CBA/N (H-2^k) mice were injected intraperitoneally with allogeneic splenocytes from BALB/c (H-2^d) mice, and then administered with combinations of anti-IL-2Ralpha, anti-IL-2Rbeta and anti-gammac mAbs or a control mAb. Addition of anti-gammac mAb together with anti-IL-2Ralpha and anti-IL-2Rbeta mAbs induced a complete inhibition of CTL response. The numbers and populations of CD4^+CD8^- and CD4^-CD8^+ T cells were not significantly affected by administration of the three anti-IL-2R mAbs, whereas NK cells were completely depleted in spleens of mice treated with the anti-IL-2R mAbs. Furthermore, skin allograft survival was also significantly prolonged by administration of the three anti-IL-2R mAbs. These results suggest that the anti-gammac mAb in combination with anti-IL-2Ralpha and anti-IL-2Rbeta mAbs is capable of suppressing induction of CTLs and NK cells, resulting in prolongation of skin allograft survival.