Analisis of Human Xenoreactive antibody and lymphocytes in porcine islet transplantation

用猪甲状腺球蛋白亲和层析法从正常人血清中纯化了天然抗体(Galα1-3Gal表位抗体)，猪胰岛细胞不与天然抗体染色，而与正常人血清染色。这些结果表明，猪胰岛细胞不表达GalA1-3Gal表位，但猪胰岛细胞中可能存在其他抗原，这些抗原是人抗体(不是抗Gal1-3Gal)的靶标。下一步，我们证明了人T细胞可以被猪刺激细胞直接激活，产生人IL-2，并在混合淋巴细胞反应(MLR)中增殖。在没有CD4阳性细胞的情况下，纯CD8阳性细胞在异种MLR中不会产生增殖和细胞毒反应。加入人IL-1α、IL-1β或IL-6不能恢复反应性，但人IL-2可以恢复反应性。

Natural antibody(antibody for Gal alpha 1-3 Gal epitope) were purified from normal human sera by affinity chromatography ussing porcine thyroglobulin.Porcine islet cells were not stained with these natural antibody but they were stained with normal human sera. These results demonstrate that porcine islet cells don't express Gal a1-3 Gal epitope, but some other antigen, which are targets of human antibodies(not anti -Gal 1-3 Gal), may exist in porcine islet cells.As a next step, we have shown that human T cells can be directly activated by porcine stimulator cells to produce human IL-2 and to proliferate in a mixed lymphocyte reaction(MLR). Pure CD8-positive cells, in the absence of CD4-positive cells, give no proliferative and cytotoxic response in the xenogeneic MLR.The addition of humanIL-1 alpha, IL-1 beta, orIL-6 does not restore reactivity but human IL-2 does.