Analisis of Human Xenoreactive antibody and lymphocytes in porcine islet transplantation

猪胰岛移植中人异种反应抗体和淋巴细胞的分析

• 批准号: 09671203
• 负责人: SATOMI Susumu
• 金额: $ 2.24万
• 依托单位: TOHOKU University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671203/
• 关键词: Gal alpha 1-3 Gal; CD8; CD4; porcine islet cell; IL-2; ブタ膵ラ島; 異種移植; 細胞性免疫; NK細胞; cytotoxic assay; アフィニティカラム; 抗α1-3gal-抗体; ABC法

Natural antibody(antibody for Gal alpha 1-3 Gal epitope) were purified from normal human sera by affinity chromatography ussing porcine thyroglobulin.Porcine islet cells were not stained with these natural antibody but they were stained with normal human sera. These results demonstrate that porcine islet cells don't express Gal a1-3 Gal epitope, but some other antigen, which are targets of human antibodies(not anti -Gal 1-3 Gal), may exist in porcine islet cells.As a next step, we have shown that human T cells can be directly activated by porcine stimulator cells to produce human IL-2 and to proliferate in a mixed lymphocyte reaction(MLR). Pure CD8-positive cells, in the absence of CD4-positive cells, give no proliferative and cytotoxic response in the xenogeneic MLR.The addition of humanIL-1 alpha, IL-1 beta, orIL-6 does not restore reactivity but human IL-2 does.

用猪甲状腺球蛋白亲和层析法从正常人血清中纯化天然抗体（Gal α 1-3 Gal表位抗体），用正常人血清染色，而用这些天然抗体不染色猪胰岛细胞。这些结果表明，猪胰岛细胞不表达Gal α 1 -3 Gal抗原表位，但猪胰岛细胞中可能存在其他抗原，这些抗原是人抗体的靶点（而不是抗Gal α 1-3 Gal）。纯的CD 8阳性细胞，在不存在CD 4阳性细胞的情况下，在异种MLR中不产生增殖和细胞毒性反应。加入人IL-1 α、IL-1 β或IL-6不能恢复反应性，但人IL-2可以。