Study on immunohistochemical staining of laminin correlation with liver metastasis

层粘连蛋白免疫组化染色与肝转移的相关性研究

• 批准号: 05671100
• 负责人: KUBO Ryuichi
• 金额: $ 1.34万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671100/
• 关键词: colorectal cancer; laminin; liver metastasis; basement membrane; ラミニン

In immunohisochemical study, we have revealed that 35% of colorectal cancer is laminin staining positivity in basement membrane (BM). And we have found that basement membrane producing cancer (BMPC) have been higher frequency of liver metastasis than colorectal cancer without BM (non-BMPC). As for recurrence rate of liver metastasis, the former is 67%, the latter is only 8%. We have examined risk of liver metastasis using BM analysis for laminin, and indicated correlation between BMPC and liver metastasis of colorectal cancer (sensitivity : 75.6%, specificity : 85.7%, accuracy : 83.9%, negative-value : 94.2%) according to that, we could predict risk of liver metastasis.Furthermore, it was seen two types BMPC in immunohistochemical study. One is BM present around each cancer nest (type A), and other was BM around several cancer nests (type B). A significant differences were found between type A and type B in rate of liver metastasis (85.7% and 41.2%, respectively). As compared primary c … More ulture cells of colorectal cancer, we found that the BM was produced by cancer cells themselves. Next, we have examined correlation between ability of producing BM and their laminin receptor (VLA-6). The expression of VLA-6 was seen in cytoplasms of cancer cells both BMPC and non-BMPC.Therefore it might be seen that ability of producing BM was independent of laminin receptor.We performed hepatic arterial infusion therapy using mitomycin-C (MMC) and 5-fluorouracil (5-FU) in postoperative patients to protect liver metastasis. The recurrence rate of liver metastasis in hepatic arterial infusion group and in control group were 5.3% and 14.2%, respectively. In BMPC, recurrence rate of liver metastasis in hepatic arterial infusion group and in control group was 16.7% and 66.7%, respectively.In the future, for elucidated as the machanisms of the liver metastasis of colorectal cancer, we make model rat of natural liver metastasis. And we will perform immunochemical intraarterial therapy with interleukin 2 for prevention of liver metastasis. Less

在免疫组织化学研究中，我们发现35%的结直肠癌基底膜中层粘连蛋白染色呈阳性。我们发现产生基底膜的癌(BMPC)比无BM的结直肠癌(Non-BMPC)肝转移的频率更高。肝转移复发率前者为67%，后者仅为8%。我们通过对层粘连蛋白进行BM分析，发现BMPC与结直肠癌肝转移的相关性(敏感性：75.6%，特异性：85.7%，准确性：83.9%，阴性值：94.2%)，据此我们可以预测肝转移的风险。一种是存在于每个癌巢周围的BM(A型)，另一种是存在于几个癌巢周围的BM(B型)。A型和B型的肝转移率分别为85.7%和41.2%，差异有统计学意义。与原始c…相比更多的结直肠癌培养细胞，我们发现BM是由癌细胞本身产生的。接下来，我们研究了BM的产生能力与其层粘连蛋白受体(VLA-6)之间的相关性。BMPC和非BMPC的癌细胞胞浆均有VLA-6的表达，提示BM的产生能力不依赖于层粘连蛋白受体。术后患者应用丝裂霉素C(MMC)和5-氟尿嘧啶(5-FU)进行肝动脉灌注治疗，以保护肝转移。肝动脉灌注组和对照组肝转移复发率分别为5.3%和14.2%。在BMPC中，肝动脉灌注组和对照组的肝转移复发率分别为16.7%和66.7%。为进一步阐明结直肠癌肝转移的机制，我们制作了自然肝转移大鼠模型。我们将用白介素2进行免疫化学动脉内治疗，以防止肝转移。较少

项目成果

喜多岡雅典、久保隆一ほか: "大腸癌とその初代培養細胞の基底膜形成能-コラーゲン包埋法による検討" 日本外科学会雑誌. 94(8). 873 (1993)

Masanori Kitaoka，Ryuichi Kubo等人：“结直肠癌及其原代培养细胞的基底膜形成能力-使用胶原蛋白包埋法的研究”日本外科学会杂志94（8）873（1993）。

J.Hida: "The role of basement membrane in colorectal cancer invasion and liver metastasis" Cancer. 74. 223-228 (1994)

J.Hida：“基底膜在结直肠癌侵袭和肝转移中的作用”癌症。

J.Hida, R.Kubo, et al: "The role of basement membrane in coblrectal cancer invasion and liver metastasis" Cancer. 72(2). 592-598 (1994)

J.Hida、R.Kubo 等人：“基底膜在直肠癌侵袭和肝转移中的作用”癌症。

J.Hida, M.Yasutomi, et al.: "The role of basement membrane in colorectal cancer invasion and liver metastasis" Cancer. 74(2). 592-598 (1994)

J.Hida、M.Yasutomi 等人：“基底膜在结直肠癌侵袭和肝转移中的作用”癌症。

久保隆一: "基底膜形成癌と肝転移" 日本消化器外科学会雑誌. 26. 2488-2493 (1993)

久保隆一：“基底膜形成癌和肝转移”日本胃肠外科杂志 26. 2488-2493 (1993)。