Assessment of neural invasion in rectal cancer by immunohistochemical laminin staining

我们用层粘连蛋白免疫组化染色检测结直肠癌的浸润和转移。层粘连蛋白染色，神经纤维束和神经束膜明显染色。因此，我们认为它是最适合直肠癌神经浸润的染色方法。通过s - 100、银浸渍、苏木精-伊红（HE）和层粘连蛋白染色，确定最适合的直肠癌神经浸润染色方法。神经侵犯率分别为58.3%、19.4%、25.0%和47.8%。S-100染色，仅神经纤维束染色明显，神经膜模糊。因此，我们认为假阳性率增加了。银浸渍染色对会神经膜和网状纤维染色明显，难以区分会神经膜和网状纤维。I-lB染色显示神经周围膜和神经纤维束模糊，难以观察，更不清楚小神经的神经侵犯。层粘连蛋白染色对神经纤维束和神经束膜均有明显的染色，我们认为层粘连蛋白染色是最适合直肠癌神经浸润的方法。通过lam mm染色，我们将神经侵犯分为三种类型（神经周围间隙型：52.0%，神经纤维束型：l0.7%，混合型：37.3%），我们认为神经周围间隙型是直肠癌最重要的神经侵犯类型。我们比较了其他组织病理因素和神经侵犯。神经浸润与浸润深度、静脉浸润及淋巴结转移密切相关。局部复发率为16.6%。神经侵犯阳性病例局部复发率（26.7%）明显高于神经侵犯阴性病例（7.3%）。神经浸润阴性病例的累计7年生存率（77%）显著高于神经浸润阳性病例（39%）。神经侵犯被认为是直肠癌局部复发和预后的预测因素，检查神经侵犯对确定治疗策略具有重要意义。我们希望通过进一步的研究来阐明神经侵犯的机制。少

We had examined infiltration and metastasis of colorectal cancer by immunohistochemical staining of laminin. By laminin staining, both nerve fiber fascicles and perineurium are distinctly stained. Therefore, we consider that it is the most suitable staining method for neural invasion of the rectal cancer. To determine the most suitable staining method for neural invasion of the rectal cancer, we performed S-l00, Silver-impregnation, Hematoxylin-Eosin (HE) and laminin staining. The rate of neural invasion shows 58.3%, 19.4%, 25.0% and 47.8%, respectively. By S-100 staining, only nerve fiber fascicles were distinctly stained, and perineurium was obscure. Therefore, we thought that the rate of false positive was increased. By silver-impregnation staining, perineurium and reticular fibers were distinctly stained, and so it was hard to distinguish between perineurium and reticular fibers. By I-lB staining, both perineurium and nerve fiber fascicles were obscure, and it was hard to investiga … More te the neural invasion of small nerve. By laminin staining, both nerve fiber fascicles and perineurium were distinctly stained, and we considered that laininin staining was the most suitable method for neural invasion of the rectal cancer. By lam mm staining, we classified the neural invasion on three types (perineural space type : 52.0%, nerve fiber fascicle type : l0.7% and mixed type : 37.3%), and we considered that perineural space type was the most important type of neural inVasion for rectal cancer.We compared with other histopathological factors and neural invasion. Neural invasion was strongly correlated with the depth of invasion, venous invasion and lymph node metastasis. The overall local recurrence rate was 16.6%. A significantly higher frequency of local recurrence (26.7%) was observed in neural invasion positive cases, compared with neural invasion negative cases (7.3%). The cumulative 7-year survival rate for neural invasion negative cases (77%) was significantly higher than that for neural invasion positive cases (39%).Neural invasion was considered a predictor of the local recurrence and prognosis of the rectal cancer, and it is important to examine the neural invasion for determine the treatment policy. We want to add a further examination to elucidate of mechanisms of neural invasion. Less