Assessment of neural invasion in rectal cancer by immunohistochemical laminin staining

免疫组化层粘连蛋白染色评估直肠癌神经侵袭

• 批准号: 08671494
• 负责人: KUBO Ryuichi
• 金额: $ 1.66万
• 依托单位: Kinki Univ.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671494/
• 关键词: rectal cancer; neural invasion; laminin staining; 局所再発; 生存率

We had examined infiltration and metastasis of colorectal cancer by immunohistochemical staining of laminin. By laminin staining, both nerve fiber fascicles and perineurium are distinctly stained. Therefore, we consider that it is the most suitable staining method for neural invasion of the rectal cancer. To determine the most suitable staining method for neural invasion of the rectal cancer, we performed S-l00, Silver-impregnation, Hematoxylin-Eosin (HE) and laminin staining. The rate of neural invasion shows 58.3%, 19.4%, 25.0% and 47.8%, respectively. By S-100 staining, only nerve fiber fascicles were distinctly stained, and perineurium was obscure. Therefore, we thought that the rate of false positive was increased. By silver-impregnation staining, perineurium and reticular fibers were distinctly stained, and so it was hard to distinguish between perineurium and reticular fibers. By I-lB staining, both perineurium and nerve fiber fascicles were obscure, and it was hard to investiga … More te the neural invasion of small nerve. By laminin staining, both nerve fiber fascicles and perineurium were distinctly stained, and we considered that laininin staining was the most suitable method for neural invasion of the rectal cancer. By lam mm staining, we classified the neural invasion on three types (perineural space type : 52.0%, nerve fiber fascicle type : l0.7% and mixed type : 37.3%), and we considered that perineural space type was the most important type of neural inVasion for rectal cancer.We compared with other histopathological factors and neural invasion. Neural invasion was strongly correlated with the depth of invasion, venous invasion and lymph node metastasis. The overall local recurrence rate was 16.6%. A significantly higher frequency of local recurrence (26.7%) was observed in neural invasion positive cases, compared with neural invasion negative cases (7.3%). The cumulative 7-year survival rate for neural invasion negative cases (77%) was significantly higher than that for neural invasion positive cases (39%).Neural invasion was considered a predictor of the local recurrence and prognosis of the rectal cancer, and it is important to examine the neural invasion for determine the treatment policy. We want to add a further examination to elucidate of mechanisms of neural invasion. Less

用免疫组织化学层粘连蛋白染色检测结直肠癌的浸润和转移。层粘连蛋白染色，神经纤维束和神经膜均清晰可见。因此，我们认为它是直肠癌神经侵袭最合适的染色方法。为确定直肠癌神经侵袭的最佳染色方法，我们进行了S-100、银染、苏木精-伊红(HE)和层粘连蛋白(LN)染色。神经侵袭率分别为58.3%、19.4%、25.0%和47.8%。S-100染色仅见神经纤维束明显染色，神经膜模糊。因此，我们认为假阳性率有所增加。经浸银染色，神经膜和网状纤维染色清晰，难以区分。I-lb染色可见神经束和神经纤维束均不明显，很难观察…。更多的是小神经侵犯。通过层粘连蛋白染色，神经纤维束和神经膜均有明显的染色，我们认为层粘连蛋白染色是判断直肠癌神经侵犯最合适的方法。通过LAM-MM染色将直肠癌的神经侵犯分为三种类型(神经周间隙型占52.0%，神经纤维束状型占10.7%，混合型占37.3%)，认为神经周围间隔型是直肠癌最主要的神经侵犯类型，并与其他组织病理学因素和神经侵犯进行了比较。神经侵犯与肿瘤侵犯深度、静脉侵犯和淋巴结转移密切相关。总的局部复发率为16.6%。神经侵袭阳性病例局部复发率(26.7%)明显高于神经侵袭阴性病例(7.3%)。神经侵袭阴性病例的累积7年生存率(77%)显著高于神经侵袭阳性病例(39%)，神经侵袭被认为是预测直肠癌局部复发和预后的指标，了解神经侵袭情况对制定治疗策略具有重要意义。我们想增加一项进一步的检查，以阐明神经侵袭的机制。较少

项目成果

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Norikazu Machidera：“层粘连蛋白的免疫组织化学对直肠癌的神经侵袭”，日本结肠直肠学会杂志 50・2（1997）。

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久保隆一：“结直肠癌胃肠癌手术中皮肤切口和手术视野的改进”《临床外科》50・12（1995）。

N,Machidera, R.Kubo, et al.: "Assessment of Neural Invasion in Rectal Cancer by Immunohistochemical Laminin Staining" J Jpn Soc Coloproctol. 50(2). 87-100 (1997)

N，Machidera，R.Kubo 等人：“通过免疫组织化学层粘连蛋白染色评估直肠癌神经侵袭”J Jpn Soc Coloproctol。

久保 隆一: "直腸癌の局所再発からみた側方リンパ筋郭清の意義" 消化器科. 25・2. 200-204 (1997)

久保龙一：“从直肠癌局部复发的角度来看外侧淋巴肌切除的意义”胃肠病学25・2（1997）。