Studies on the mechanism of osteopenia associated with abnormal endocrine conditions

内分泌异常引起骨质减少的机制研究

• 批准号: 05671220
• 负责人: HOTOKEBUCHI Takao
• 金额: $ 1.28万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671220/
• 关键词: Endocrine abnormality; Growth hormone; Growth factor; Thyroid hormone; Fracture; 骨粗鬆症; ホルモン; 遺伝子発現

We studied the relationship between a systemic factor, growth hormone, and local peptide growth factors in the skeletal system in normal rats. Human recombinant growth hormone was injected subcutaneously in 4-week-old rats, and sequential changes of serum growth hormone and gene expression of growth factors were analyzed. Serum growth hormone showed a peak 6 hours after the initial injection. Systemic administration of growth hormone slightly decreased mRNA for transforming growth factor-beta (TGF-beta) in femoral bones 6 hours after the initial injection, but the mRNA returned to the initial level 12 hours after the initial injection. However, there was no statistical significance. On the other hand, mRNAs for basic fibroblast growth factor (bFGF) and insulin-like growth factor-I (IGF-I) increased in response to growth hormone in costal cartilage. These data suggest that local peptide growth factors are regulated by systemic growth hormone.13EA02 : In the second part of our study, fracture healing in the drug-induced hypothyroid rats was studied. The fracture callus had lower torsional stiffness in hypothyroid rats in mechanical testing. Depletion of thyroid hormone also suppressed chondrogenesis and endochondral ossification. Northern analysis revealed decreased expression of alkaline phosphatase and osteocalcin, which are the indices for bone formation. These data suggest the suppressed bone formation in the hypothyroid condition.

我们研究了正常大鼠骨骼系统中系统因子生长激素和局部肽生长因子之间的关系。给4周龄大鼠皮下注射重组人生长激素，分析血清生长激素和生长因子基因表达的时序变化。血清生长激素在首次注射后6小时出现高峰。在初次注射后6小时，全身给予生长激素略微降低了股骨中转化生长因子-β（TGF-β）的mRNA，但在初次注射后12小时，mRNA恢复到初始水平。但无统计学意义。另一方面，在肋软骨中，碱性成纤维细胞生长因子（bFGF）和胰岛素样生长因子-I（IGF-I）的mRNA响应于生长激素而增加。这些数据表明，局部肽类生长因子受全身生长因子的调节。13 EA 02：在我们研究的第二部分，研究了药物诱导的甲状腺功能减退大鼠的骨折愈合。在力学测试中，甲减大鼠骨折骨痂的扭转刚度较低。甲状腺激素的耗竭也抑制软骨发生和软骨内骨化。北方分析显示碱性磷酸酶和骨钙素的表达减少，这是骨形成的指标。这些数据表明在甲状腺功能减退的情况下骨形成受到抑制。

项目成果

A.Iwaki: "Localization and quantification of proliferating cells during rat fracture repair" Orthopedic Transaction. (発表予定). (1994)

A.Iwaki：“大鼠骨折修复过程中增殖细胞的定位和定量”《骨科学报》（即将出版）。

神宮司誠也: "Basic fibroblast growth factor及びTransforming growth factorβの骨折治癒過程における細胞増殖制御への関与." 骨折. 16. 5-8 (1994)

Seiya Jingu Tsukasa：“碱性成纤维细胞生长因子和转化生长因子 β 参与骨折愈合过程中细胞增殖的控制。” 骨折。

Izumi,T.: "Growth hormone modulated the gene expression of growth factors in rat cosatal cartilage in vivo." Orthop.Trans.18. 458-459 (1994)

Izumi,T.：“生长激素调节大鼠体内肋骨软骨中生长因子的基因表达。”

Jingushi, S.: "A combination treatment with basic fibroblast growth factor and perichondrium autograft for a full-thickness articular cartilage defect." Orthop.Trans.18. 461-462 (1994)

Jingushi, S.：“碱性成纤维细胞生长因子和自体软骨膜移植物联合治疗全层关节软骨缺损。”

Shida,J.: "A single injection of basic FGF stimulates articular cartilage enlargement in rat knee joints." Orthop.Trans.18. 461- (1994)

Shida,J.：“单次注射碱性 FGF 可刺激大鼠膝关节的关节软骨增大。”