The Study of The Mechanisms of Onset and Progression of Rapidly Dystructive Coxarthrosis

快速破坏性髋关节炎发病及进展机制的研究

• 批准号: 13671518
• 负责人: HOTOKEBUCHI Takao
• 金额: $ 2.18万
• 依托单位: Saga Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671518/
• 关键词: osteoclast; Rapidly dystructive coxarthrosis; cell culture; 変形性関節症; 骨膜細胞; オピオイド

[PURPOSE]Rapidly dystructive coxathrosis (RDC) induces severe disorder of activities of daily living resulted from rapidly progressive hip dystruction. RDC is characterized by the clinical corse that progress more rapidly than that of osteoarthrosis (OA) and rheumatoid arthritis (RA). Osteoclasts are specialized to carry out bone resorption in physiological or pathological conditions. In this study, we analyzed the consistent of osteoclasts in RDC using synovial cell culture system.[METHODS and RESULTS]1. Examination of osteodastogenesis in the synovial cell culture.We investigated the human synovial cell culture system. Synovial cells isolated front patients with RDC and OA using enzyme digestion were cultured for various periods with/without some growth factors and cytokines. Osteoclast-like multinucleated cells were formed after treated for 2weeks. This culture system was considered to be useful for analyzing a consistent of osteoclasts in joint dislorders.2. Isolation and analysis … More of mature osteoclasts from RDC patients.Osteoclast-like cells were present at early phase in the culture of synovial cells derived from RDC, but not from OA synovium. Now we examine the phenotypes and functional analysis about these cells.3. Analysis of factors influences the formation and function of osteoclasts in the culture of synovial cells.(1) To determine the activities of synovial stromal cells and osteoclast-progenitor cells, we have examined co-culture system using some cell lines. Now we analyze the expression of RANKL mRNA in UMR106 cell, and the activity of osteoclast formation in the cultures of RAW264.7 cell or THP-I cell.(2) We investigated the effects of conditioned medium collected Cram RDC and OA synovial cell culture to osteoclastogenesis Conditioned medium derived from OA inhibited the formation of osteoclasts by dose-dependent manner in RAW264.7 cells induced by RANKL. Non we investigate the mechanisms of inhibitory effect of that from OA and test an effect of that from RDC. Less

【目的】快速进行性髋关节损伤（RDC）可导致严重的日常生活活动障碍。RDC的特点是临床病程比骨关节病（OA）和类风湿性关节炎（RA）进展更快。破骨细胞是在生理或病理条件下进行骨吸收的专门细胞。本研究利用滑膜细胞培养系统分析了破骨细胞在RDC中的一致性。【方法与结果】滑膜细胞培养中成骨细胞生成的检查。我们研究了人滑膜细胞培养系统。采用酶消化法分离的前部RDC和OA患者的滑膜细胞，在不同时期进行有/无生长因子和细胞因子的培养。2周后形成破骨细胞样多核细胞。该培养系统被认为可用于分析关节紊乱中破骨细胞的一致性。RDC患者成熟破骨细胞的分离与分析在RDC衍生的滑膜细胞培养的早期阶段存在破骨细胞样细胞，但在OA滑膜中不存在。现在我们对这些细胞进行表型分析和功能分析。滑膜细胞培养中影响破骨细胞形成及功能的因素分析。(1)为了确定滑膜基质细胞和破骨细胞祖细胞的活性，我们用一些细胞系检测了共培养系统。现在我们分析RANKL mRNA在UMR106细胞中的表达，以及RAW264.7细胞和THP-I细胞的破骨细胞形成活性。(2)研究了Cram RDC和OA滑膜细胞培养条件培养基对破骨细胞形成的影响。OA条件培养基对RANKL诱导的RAW264.7细胞破骨细胞形成具有剂量依赖性。我们研究了OA的抑制作用机制，并检验了RDC的抑制作用。少