The basic study of the electrofertilization using an electrofusion technique

电融合技术电受精的基础研究

基本信息

批准号：
05671346
负责人：
SENGOKU Kazuo
金额：
$ 1.02万
依托单位：
ASAHIKAWA MEDICAL COLLEGE
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671346/
关键词：
electrofusion subzonal insemination

项目摘要

We studied the probability of the successful fertilization using an electrofusion methods, and obtained the following results.1.In the experiments that three to ten mouse sperms were injected into the perivitelline space of each mouse oocytes, the best results of sperms attachment, fertilization were obtained after application of dc of 3.5KV/cm, 10musec followed by ac pulses (2MHz, 100V/cm, 20sec each). After pulse application, 25.5% of the oocytes were fertilized. However, 90% of the oocytes showed the polyspermic fertilization and 15.5% of the oocytes were degenerated.2.Oocyte activation prior to application of fusion pulse is known to facilitate oocyte-sperm fusion. When the oocytes were electroactivated by exposures to ac pulse (2MHz, 50V/cm, 10sec) and followed by dc (1.5KV/cm, 50musec), about 70% of the oocytes were activated. Twenty percents of the oocytes stimulated with electoactivation developed to the pronucleus stage after the application of the electrofusion. However, all these oocytes were arrested at the PN stage.3.When a single mouse sperm was injected into the perivitelline space and applied an electrofusion technique, 12.5% of these oocytes were fertilized and developed to the PN stage.4.In the trial using human sperms and hamster oocytes, 3.0% of the hamster oocytes were ferilized under the same conditions of electrofusion. However, all oocytes were arrested at the PN stage and 15.0% of the oocytes were degenerated.Although the success rate is very low and more study about the best conditions for electrofusion is needed, our preliminary results may offer important prospects of the treatment of the patients with sperm dysfunction.

我们研究了使用电渗漏方法成功进行施肥的可能性，并获得了以下结果。1。在实验中，将三到十个小鼠精子注入每个小鼠卵母细胞的细胞素空间中，精子附着的最佳结果，在3.5kv/cm，10mus，ACM/acss culs ack aft fullafe fulpects，获得了100次（2mus）的最佳结果。每个）。脉冲施用后，将25.5％的卵母细胞受精。然而，将90％的卵母细胞显示出多性经体受精，15.5％的卵母细胞被退化。2.EOCYTE激活在应用融合脉冲之前已知可以促进卵母细胞融合。当卵母细胞通过暴露于AC脉冲（2MHz，50V/cm，10sec），然后进行DC（1.5kV/cm，50musec）进行反激活时，约70％的卵母细胞被激活。施用电催化后，通过选举刺激的卵母细胞刺激了二十个卵母细胞。然而，所有这些卵母细胞在PN阶段被捕。3。将单个小鼠精子注入了细胞环素空间并应用了电渗漏技术时，这些卵母细胞中的12.5％被施肥并开发到PN阶段。4。在使用人类精子和Hamster Oocys的试验中，将其用于hamster efirof firof firof firof ham sers the ham ecy the the the pn阶段。然而，所有卵母细胞在PN阶段被捕，并将15.0％的卵母细胞退化。尽管成功率非常低，并且需要对需要进行电催化的最佳条件进行研究，但我们的初步结果可能会为患者患者患有精子功能障碍的患者提供重要的前景。