The basic study of xenotransplantation of encapsulated cryopreserved mouse immature follicles

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We studied the possibility of preservation of immature ovarian follicles using microbeads methods. The cryopreserved mouse primordial was encapsulated in agarose or three-layer microbeads, and microbeads were xenogenically implanted in the rats. The following results were obtained.1. Mouse primordial follicles were successfully cryopreserved with slow freeze-rapid thaw protocols. Moreover, the supplementation of free radical scavengers, particularly combinations of SOD with NO scavengers in freezing and thawing media, improved the post-thaw survival and fertilization rates of cryopreserved mouse oocytes.2. The microbeads encapsulating immature follicles retain biocompatibility after homotransplantations, suggesting possible future application of this methods for preservation immature follicles. However, after xenotransplantation, viable follicles and follicular growth were not obtained.3. The expression of mRNA of GDF9 and C-kit in the immature oocytes was confirmed.Although successful xenotransplantation was not obtained using encapsulated cryopreserved mouse immature follicles, our preliminary results may offer important aspects of future applications of this microbeads method for ovarian tissues banking.