A Study on Metabolism of Extracelluar Matrix in the fetal membrane

1.孕妇血清中TIMP浓度在妊娠期间无明显变化。它也没有显示出任何显着差异，非妊娠血清。在胎膜早破和早产病例中，血清TIMP水平无明显差异，但羊水中TIMP水平明显高于母血、羊水中TIMP水平（P<0.01）。免疫组化显示TIMP在胎膜、羊膜上皮、羊膜成纤维细胞、绒毛膜上皮及脱落细胞中均有明显定位. MMP-1在孕晚期升高，尤其是在宫颈成熟初期。分娩时羊水中MMP-1水平与母血、脐血中MMP-1水平相同。免疫组化法检测MMP-1与TIMP一样，在胎膜、羊膜上皮、羊膜成纤维细胞、绒毛膜上皮和蜕膜中均有明确的定位.本实验观察了IL-1、IL-2、IL-6、TNF对体外培养的人羊膜细胞和绒毛膜细胞产生基质金属蛋白酶-1（MMP-1）和组织金属蛋白酶组织抑制剂-1（TIMP-1）的影响，发现IL-1、IL-2、IL-6可促进绒毛膜细胞产生MMP-1和TIMP-1。IL-1、IL-2、IL-6、TNF可促进羊膜细胞TIMP-1的生成，但不影响MMP-1的生成。

1. TIMP concentration of maternal serum didn't show any significant changes dur-ing pregnancy. It either didn't show any significant differences to non-pregnant serum. In the cases of PROM and premature labor, there were not any difference in serum levels of TIMP.The level of TIMP at delivery in amni-otic fluid was significanty (p<0.01) higher than that of maternal serum, um-bilical serum. TIMP was clearly localized in fetal membrane, amniotic epithelium, amniotic fibroblast, chorionic epithelium and de-cidua, by immunohistochemical method.2. MMP-1 concentration of maternal serum was increased at the 3rd trimester.especially at the start of cervical maturation. The level of MMP-1 at delivery in amniotic fluid was same level to that of maternal serum, um-bilical serum..MMP-1 was clearly localized, like TIMP,in fetal membrane, amniotic epithelium, amniotic fibroblast, chorionic epithelium and decidua, by immunohistochemical method.3. The effects of IL-1, LI-2, IL-6, TNF on the production of matrix metalloproteinases-1, TIMP-1 in cultured human amniotic and chorionic cells, were examined.In chorionic cells, IL-1, IL-2, IL-6 enhanced the production of MMP-1 and TIMP-1. TNF,also, enhanced the production of MMP-1, but supressed TIMP-1 production.In amniotic cells, IL-1, IL-2, IL-6, TNF enhanced the production of TIMP-1, but MMP-1 was not found.