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A Study on Metabolism of Extracelluar Matrix in the fetal membrane

胎膜细胞外基质代谢的研究

基本信息

批准号：
05671358
负责人：
SOEDA Yoshikatsu
金额：
$ 1.22万
依托单位：
TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671358/
关键词：
MMP-1 TIMP-1 PROM Fetal Membrame Matrix MMP_s MMPs

项目摘要

1. TIMP concentration of maternal serum didn't show any significant changes dur-ing pregnancy. It either didn't show any significant differences to non-pregnant serum. In the cases of PROM and premature labor, there were not any difference in serum levels of TIMP.The level of TIMP at delivery in amni-otic fluid was significanty (p<0.01) higher than that of maternal serum, um-bilical serum. TIMP was clearly localized in fetal membrane, amniotic epithelium, amniotic fibroblast, chorionic epithelium and de-cidua, by immunohistochemical method.2. MMP-1 concentration of maternal serum was increased at the 3rd trimester.especially at the start of cervical maturation. The level of MMP-1 at delivery in amniotic fluid was same level to that of maternal serum, um-bilical serum..MMP-1 was clearly localized, like TIMP,in fetal membrane, amniotic epithelium, amniotic fibroblast, chorionic epithelium and decidua, by immunohistochemical method.3. The effects of IL-1, LI-2, IL-6, TNF on the production of matrix metalloproteinases-1, TIMP-1 in cultured human amniotic and chorionic cells, were examined.In chorionic cells, IL-1, IL-2, IL-6 enhanced the production of MMP-1 and TIMP-1. TNF,also, enhanced the production of MMP-1, but supressed TIMP-1 production.In amniotic cells, IL-1, IL-2, IL-6, TNF enhanced the production of TIMP-1, but MMP-1 was not found.

1.母血TIMP浓度在孕期无明显变化。与非妊娠血清相比，两者均无显著差异。胎膜早破与早产孕妇血清TIMP水平无明显差异，羊水中TIMP水平显著高于母血、脐血TIMP水平(P<0.01)。结论：1.免疫组织化学方法显示TIMP在胎膜、羊膜上皮、羊膜成纤维细胞、绒毛上皮和绒毛膜中有明显的表达。母体血清中的基质金属蛋白酶-1浓度在妊娠晚期升高，尤其是在宫颈成熟开始时。羊水中的基质金属蛋白酶-1水平与母体血清、脐血清中的水平相同。免疫组织化学方法显示，胎膜、羊膜上皮、羊膜成纤维细胞、绒毛膜上皮和蜕膜中的基质金属蛋白酶-1与TIMP一样有明显的定位。观察IL-1、IL-2、IL-6、TNF对人羊膜和绒毛细胞产生基质金属蛋白酶-1、TIMP-1的影响。在绒毛细胞中，IL-1、IL-2、IL-6促进基质金属蛋白酶-1、TIMP-1产生。在羊膜细胞中，IL-1、IL-2、IL-6、TNF促进TIMP-1的产生，但未见有MMP1的表达。