特发性眼眶炎性假瘤（idiopathic orbital inflammatory pseudotumor，IOIP）是一种以炎细胞浸润和纤维化为病理特点的常见眼眶病。多数IOIP因纤维化程度高，治疗效果差，复发率高，揭示其发病机制已成为亟待解决的重要问题。IOIP发病机制研究虽已广泛开展，但以长非编RNA（lncRNA）为代表的表观遗传研究尚未深入阐明。前期工作表明，PAUPAR是与IOIP发生和纤维化密切相关的lncRNA，纤维化相关因子TIMP-1为其下游靶点。据此本项目提出了PAUPAR调控TIMP-1基因表达参与IOIP纤维化发生的研究假设。本项目拟采用染色质免疫共沉淀、环状染色质构象捕获等技术，探究PAUPAR对TIMP-1基因修饰机制，揭示参与修饰的关键蛋白，全面辨析PAUPAR在IOIP发生中的调控模式，期望为以纤维化为病理特征的IOIP的研究提供理论依据。

Idiopathic orbital inflammatory pseudotumor (IOIP) is a common orbital disease characterized by inflammatory cell infiltration and fibrosis. Most IOIP patients have a high degree of fibrosis, poor treatment outcome and high recurrence rate, revealing the pathogenesis has become an important issue to be solved. Although studies on the pathogenesis of IOIP have been widely carried out, the epigenetic studies represented by long non-coding RNA (lncRNA) have not yet been elucidated. In preliminary work,we have confirmed PAUPAR lncRNA was closely related to IOIP and fibrosis, and TIMP-1 highly related to fibrosis was the downstream of PAUPAR. Based on these findings, we propose the hypothesis that PAUPAR lncRNA determines IOIP fibrosis by modification of TIMP-1 gene expression.With Chromatin Immunoprecipitation and chromosome conformation capture-on-chip technologies,this project aims to explore the mechanism of PAUPAR lncRNA regulating epigenetics modification of TIMP-1 expression, reveal the key protein factor during modification, expound the regulation feature of PAUPAR in IOIP, as well as provide theoretical basis for IOIP.