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Basic study on the demage of retinal pigment epithelium and the treatment with retinal transplantation

视网膜色素上皮损伤及视网膜移植治疗的基础研究

基本信息

批准号：
05671454
负责人：
YAMADA Takahiko
金额：
$ 1.34万
依托单位：
Tohoku university
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

We have been studying on the retina with artificially danaged retinal pibment epithelium (RPE). In this year, in order to evaluate the influence of transplanted RPE on the neural retina under the pathological condition, we examined localization of neurotransmitters in the rabbit retina which has artificially damaged RPE with or without transplanted RPE.Performing vitreous surgery, an artificial retinal detachment and an abrasion of RPE were produced in albino rabbit eyes and detached retinas were reattached with or without transplantation of RPE cells obtained from pigmented rabbit eyes. The fundus was examinated periodically and each eyes were enucleated at one, two, three and four weeks after the surgery. Each eye was fixed with 2.5% glutaraldehyde solution and immunohistochemical staining was performed with anti-GABA anti-glycine and anti-glutamate antibody. In experimental eyes without transplantation positive staining for glycine were recognized in photoreceptor cell layr although GABA and glutamate showed normal localization. In experimental eyes with transplantation this abnormal distribution of immunoreactive glycine was not recognized around the transplanted RPE cells. This abnornal distribution was seen one week after the surgery and became weak four week after the surgery. The abnormal distributions of glycine like immuno-reactivity was prevented by the transplanted RPE under the pathological condition. This effect might be some sort of rescue caused by RPE transplantation.

本实验对人工损伤视网膜色素上皮（retinal pibment epithelium，RPE）的视网膜进行了研究。在这一年中，为了评估在病理条件下移植的RPE对神经视网膜的影响，我们检查了在有或没有移植RPE的情况下人工损伤RPE的兔视网膜中神经递质的定位。在白化病兔眼上造成人工视网膜脱离和RPE磨损，并在有或无RPE细胞移植的情况下将脱离的视网膜复位从着色的兔子眼睛中获得。术后1、2、3、4周分别行眼底检查和眼球摘除术。每只眼用2.5%戊二醛溶液固定，用抗GABA、抗甘氨酸和抗谷氨酸抗体进行免疫组织化学染色。在未移植的实验眼中，虽然GABA和谷氨酸显示正常的定位，但在感光细胞层中识别出甘氨酸阳性染色。在移植的实验眼中，这种免疫反应性甘氨酸的异常分布在移植的RPE细胞周围没有被识别。这种异常分布在术后1周出现，术后4周减弱。病理状态下移植的RPE可防止甘氨酸样免疫反应性的异常分布。这种效应可能是由RPE移植引起的某种拯救。