The Effects of Cytoskeltal Proteins And Insulin-like Growth Factor II on Invasive Capacity of Human Neuroblastoma Cells.

细胞骨架蛋白和胰岛素样生长因子 II 对人神经母细胞瘤细胞侵袭能力的影响。

基本信息

批准号：
05671496
负责人：
ZAIZEN Yoshio
金额：
$ 1.41万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671496/
关键词：
Neuroblastoma Invasion Insulin-like growth factor II Retinoic acid N-myc Cellular motility 細胞骨格蛋白

项目摘要

Insulin-like growth factor II (IGF-II) is implicated in the development of the vertebrate neural circuitry, and increases neurite growth in vitro and in vivo. We examined the relationship of IGF-II expression to the in vitro differentiation induced by retinoic acid (RA). We find that RA stimulates an increase in IGF-II messenger RNA (mRNA) in the SK-N-SH (SH) neuroblastoma cell kine. An increase of IGF-II mRNA is detected within 12 h of treatment and precedes morphological differentiation. A RA dose response test indicates that an increase in IGF-II mRNA occurs within 2 days in SH cells treated with doses of RA from 10^<-8> to 10^<-5> M.We suggest that IGF-II expression may be RA in vitro and may lead to neuroblastoma differentiation.The invasiveness of IMR-32 and GOTO,of which amplification of N-myc gene was 15 and 12 copies respectively, was 9.1 times and 8.8 times higher than that of SK-N-SH without N-myc amplification. Using a video-imaging analysis, we directly measured the motility of neuroblastoma cells on culture flask. These trace images clearly show that the motility of IMR-32 and GOTO is 2.3-1.7 times higher than that of SK-N-SH which had extremely low invasiveness and motility. To clarify the effect of reduction in N-myc gene expression on invasive capacity and cellular motility, GOTO was treated 10^<-5> MRA for 72 hours before examination. RA treatment markedly reduced the N-myc gene expression in approximately one quarter of the control and apparently decreased invasive capacity of GOTO as well as cellular motility which declined about one fifth of untreated GOTO.

胰岛素样生长因子II（IGF-II）与脊椎动物神经回路的发展有关，并在体外和体内增加神经突的生长。我们检查了视黄酸（RA）引起的IGF-II表达与体外分化的关系。我们发现RA刺激了SK-N-SH（SH）神经母细胞瘤细胞束中IGF-II信使RNA（mRNA）的增加。在治疗后12小时内检测到IGF-II mRNA的增加，并在形态学分化之前。 RA剂量反应测试表明，IGF-II mRNA的增加发生在2天内，在用RA剂量从10^<-8>到10^<-5> m的SH细胞中发生，M.我们表明IGF-II的表达可能是在体外ra的，并且可能导致神经母细胞瘤的分化。时间，比没有N-MYC扩增的SK-N-SH高8.8倍。使用视频成像分析，我们直接测量了神经母细胞在培养瓶中的运动。这些痕迹图像清楚地表明，IMR-32和GOTO的运动性比SK-N-SH的运动性高2.3-1.7倍，SK-N-SH具有极低的侵入性和运动性。为了阐明N-MYC基因表达对浸润能力和细胞运动性的降低的影响，在检查前将GoTo处理10^<-5> MRA 72小时。 RA治疗显着降低了N-MYC基因的表达，大约四分之一的对照，显然降低了GOTO的浸润能力以及细胞运动性，而细胞运动性下降了约五分之一未经治疗的GOTO。